Aim To purify hepatocyte regeneration stimulatory factor from shark liver and research its molecular feature and activity. Methods and Results Hepatocyte regeneration stimulatory factor (sHRSF) was isolated from healt...Aim To purify hepatocyte regeneration stimulatory factor from shark liver and research its molecular feature and activity. Methods and Results Hepatocyte regeneration stimulatory factor (sHRSF) was isolated from healthy shark livers and separated by homogenization, freezing-melting, heat treating, centfifugation, and ultmfdwation. HRSF activity was found mainly in the subfraction of molecular weight less than 30 000 daltons. This crude ultrafihrate was further purified successively by DEAE-Sepharose fast flow chromatography, FPLC Resource 30Q, Resource Q and Mono Q chromatography.A single band was displayed on sodium dodecyl sulfate polyacrylamide gel electrophoresis, which corresponds to molecular weight of 14 600 daltons. The characteristic absorption was obtained at the wavelength 276 nm. The isoelectric point was about 5.1. It contained 18 amino acids and the 15 N-terminal amino acid residues were LVGPIGAVGPAGKDG. It had a significant activity in stimulating liver to regenerate. Condusion We obtained an unknown new active protein, that is hepatocyte regeneration stimulatory factor from shark liver ( sHRSF).展开更多
The effects of the varying concentrations of the polysaccharide, eletrolyte and different pH values and temperature on the viscosity of the exopolysaccharide produced by \%Aphanothece halophytica \%Fremy were analysed...The effects of the varying concentrations of the polysaccharide, eletrolyte and different pH values and temperature on the viscosity of the exopolysaccharide produced by \%Aphanothece halophytica \%Fremy were analysed. The results showed that its intrinsic viscosity is 9.76 dl/g, which provides good stability to salt, pH and temperature.展开更多
基金NationalMarine863Project (No .2 0 0 1AA62 40 90),NationalNaturalScienceFoundationofChina (No .3 0 17110 3 )
文摘Aim To purify hepatocyte regeneration stimulatory factor from shark liver and research its molecular feature and activity. Methods and Results Hepatocyte regeneration stimulatory factor (sHRSF) was isolated from healthy shark livers and separated by homogenization, freezing-melting, heat treating, centfifugation, and ultmfdwation. HRSF activity was found mainly in the subfraction of molecular weight less than 30 000 daltons. This crude ultrafihrate was further purified successively by DEAE-Sepharose fast flow chromatography, FPLC Resource 30Q, Resource Q and Mono Q chromatography.A single band was displayed on sodium dodecyl sulfate polyacrylamide gel electrophoresis, which corresponds to molecular weight of 14 600 daltons. The characteristic absorption was obtained at the wavelength 276 nm. The isoelectric point was about 5.1. It contained 18 amino acids and the 15 N-terminal amino acid residues were LVGPIGAVGPAGKDG. It had a significant activity in stimulating liver to regenerate. Condusion We obtained an unknown new active protein, that is hepatocyte regeneration stimulatory factor from shark liver ( sHRSF).
文摘The effects of the varying concentrations of the polysaccharide, eletrolyte and different pH values and temperature on the viscosity of the exopolysaccharide produced by \%Aphanothece halophytica \%Fremy were analysed. The results showed that its intrinsic viscosity is 9.76 dl/g, which provides good stability to salt, pH and temperature.
