A method is described which permits transmission electron microscope of single cells treated with HpD plus laser microirradiation. The preselected single cell that was irradiated by laser under light microscope and fo...A method is described which permits transmission electron microscope of single cells treated with HpD plus laser microirradiation. The preselected single cell that was irradiated by laser under light microscope and followed fixation, embedded and sectioning is examined under electron microscope. The results demonstrated that at the light dose of 1.88 ml/μm2 not only the irradiated nucleolus appeared transparent region, but the other parts such as non-irradiated mitochondria in cytoplasm can also be damaged. When partial cytoplasm is irradiated with the light dose of 4.50 ml/μm2, the damages appear in all cytoplasm, but there is little change in the nucleus. The experimental results also demonstrate that cytoplasm is more sensitive than nucleus. It is the mitochondria in cytoplasm that are very sensitive to HpD plus laser.展开更多
Since HPD in combination with light for treatment of malignancy was used by Dougherty et al. (1978), recent progress of photodynamic therapy using laser as light source to excite HPD both in diagnosis and therapy of c...Since HPD in combination with light for treatment of malignancy was used by Dougherty et al. (1978), recent progress of photodynamic therapy using laser as light source to excite HPD both in diagnosis and therapy of cancer is currently receiving consid-展开更多
文摘A method is described which permits transmission electron microscope of single cells treated with HpD plus laser microirradiation. The preselected single cell that was irradiated by laser under light microscope and followed fixation, embedded and sectioning is examined under electron microscope. The results demonstrated that at the light dose of 1.88 ml/μm2 not only the irradiated nucleolus appeared transparent region, but the other parts such as non-irradiated mitochondria in cytoplasm can also be damaged. When partial cytoplasm is irradiated with the light dose of 4.50 ml/μm2, the damages appear in all cytoplasm, but there is little change in the nucleus. The experimental results also demonstrate that cytoplasm is more sensitive than nucleus. It is the mitochondria in cytoplasm that are very sensitive to HpD plus laser.
基金Supported by the State Scientific and Technological Commission of China
文摘Since HPD in combination with light for treatment of malignancy was used by Dougherty et al. (1978), recent progress of photodynamic therapy using laser as light source to excite HPD both in diagnosis and therapy of cancer is currently receiving consid-
