目的研究决明子的高效液相色谱(HPLC)特征图谱,为科学评价省评价性抽验品种决明子的质量现状提供依据。方法特征图谱采用Waters Sun Fire TM C18(250 mm×4.6 mm,5μm)色谱柱,以乙腈-磷酸溶液为流动相梯度洗脱。色谱数据采用相似度...目的研究决明子的高效液相色谱(HPLC)特征图谱,为科学评价省评价性抽验品种决明子的质量现状提供依据。方法特征图谱采用Waters Sun Fire TM C18(250 mm×4.6 mm,5μm)色谱柱,以乙腈-磷酸溶液为流动相梯度洗脱。色谱数据采用相似度评价软件分析并对结果进行聚类分析,探讨特征图谱与质量的相关性。结果分别建立了决明及炒决明的HPLC特征图谱;聚类分析结果表明,市场上的决明子质量参差不齐,相似度与现行药典标准含量测定合格率有很大相关性。结论决明子总体质量较差,质量标准有待商榷。建立的特征图谱能清晰反映决明子生品和炮制品的真实质量,可用于该药的质量控制。展开更多
In the present study,we studied the changes of the contents of alkaloids in the herbaceous stems of Ephedra sinica during different harvest periods as well as before and after processing.The alkaloid contents of 39 ba...In the present study,we studied the changes of the contents of alkaloids in the herbaceous stems of Ephedra sinica during different harvest periods as well as before and after processing.The alkaloid contents of 39 batches of ephedra herb,prepared slices ephedra and honey-fried ephedra,24 batches of ephedra herb with different harvesting periods,which were all collected from cultivation base in Inner Mongolia,and 38 batches of prepared slices ephedra purchased from the market were detected by taking norephedrine(NE),norpseudoephedrine(NPE),ephedrine(E),pseudoephedrine(PE)and methylephedrine(ME)as indicators by using HPLC method.The content of total alkaloid in prepared slices ephedra(1.71%–3.14%)was higher than that in ephedra herb(1.20%–2.53%)and honey-fried ephedra(1.52%–2.99%).Contents of different alkaloids in these three types of samples were significantly different.Prepared slices ephedra and honey-fried ephedra showed significant differences in the contents of NE,NPE and ME(P<0.05),and the contents of E were significantly different between ephedra herb and honey-fried ephedra(P<0.05).The total alkaloid content of ephedra herb was the highest in September(3.10%).Alkaloid contents of prepared slices ephedra collected in the market were uneven and 13%–91%lower than those collected from cultivation base.The results provided a basis for the quality evaluation of ephedra herb and its processed products,and had certain guiding significance for the selection of processed ephedra according to different drug purposes in clinical application.It also provided data support for the harvesting time of ephedra herb.展开更多
文摘目的研究决明子的高效液相色谱(HPLC)特征图谱,为科学评价省评价性抽验品种决明子的质量现状提供依据。方法特征图谱采用Waters Sun Fire TM C18(250 mm×4.6 mm,5μm)色谱柱,以乙腈-磷酸溶液为流动相梯度洗脱。色谱数据采用相似度评价软件分析并对结果进行聚类分析,探讨特征图谱与质量的相关性。结果分别建立了决明及炒决明的HPLC特征图谱;聚类分析结果表明,市场上的决明子质量参差不齐,相似度与现行药典标准含量测定合格率有很大相关性。结论决明子总体质量较差,质量标准有待商榷。建立的特征图谱能清晰反映决明子生品和炮制品的真实质量,可用于该药的质量控制。
基金State Administration of Traditional Chinese Medicine Standardization Action Plan Project(Grant No.ZYBZH-Y-GD-13)
文摘In the present study,we studied the changes of the contents of alkaloids in the herbaceous stems of Ephedra sinica during different harvest periods as well as before and after processing.The alkaloid contents of 39 batches of ephedra herb,prepared slices ephedra and honey-fried ephedra,24 batches of ephedra herb with different harvesting periods,which were all collected from cultivation base in Inner Mongolia,and 38 batches of prepared slices ephedra purchased from the market were detected by taking norephedrine(NE),norpseudoephedrine(NPE),ephedrine(E),pseudoephedrine(PE)and methylephedrine(ME)as indicators by using HPLC method.The content of total alkaloid in prepared slices ephedra(1.71%–3.14%)was higher than that in ephedra herb(1.20%–2.53%)and honey-fried ephedra(1.52%–2.99%).Contents of different alkaloids in these three types of samples were significantly different.Prepared slices ephedra and honey-fried ephedra showed significant differences in the contents of NE,NPE and ME(P<0.05),and the contents of E were significantly different between ephedra herb and honey-fried ephedra(P<0.05).The total alkaloid content of ephedra herb was the highest in September(3.10%).Alkaloid contents of prepared slices ephedra collected in the market were uneven and 13%–91%lower than those collected from cultivation base.The results provided a basis for the quality evaluation of ephedra herb and its processed products,and had certain guiding significance for the selection of processed ephedra according to different drug purposes in clinical application.It also provided data support for the harvesting time of ephedra herb.
