细胞焦亡对肝癌发生发展的影响及中医药的干预作用

细胞焦亡是一种新发现的程序性细胞死亡方式,在肝癌中发挥着关键作用。故拟基于细胞焦亡的分子机制及其在肝癌发病机制中的作用进行深入分析,并结合中医药在调节焦亡肝癌发展过程中的作用进行探讨,以期为临床提供新的治疗思路。

基于网络药理学探讨山茱萸治疗心源性休克的作用机制研究

目的:探究山茱萸干预心源性休克的潜在机制。方法:从TCMSP数据库获取山茱萸的有效成分和潜在靶点。收集GeneCards、OMIM、Drugbank和PharmGkb等数据库中的心源性休克相关基因。对两者取交集后找出发挥作用的化合成分和潜在靶点。对潜在靶点进行PPI、GO、KEGG分析,探究山茱萸干预心源性休克的作用机制。结果:通过TCMSP共找出山茱萸中的20个化学成分,54个靶点。综合所有疾病数据库共获得492个心源性休克相关基因。两者取交集后得到15个山茱萸干预心源性休克潜在靶点。PPI结果显示,PTGS2、ADRB2、DRD1、ADRA1A、ADRA1B、CASP3之间有较强的相互作用关系。GO结果表明,BP与腺苷酸环化酶激活肾上腺素能受体信号通路、肾上腺素能受体信号通路、管径调节的调节有关。CC与膜筏、膜微域、突触前膜的内在成分有关。MF参与G蛋白偶联胺受体活性、儿茶酚胺结合、参与凋亡信号通路的半胱氨酸型肽酶活性有关。KEGG结果显示,交集基因与钙信号通路、cGMP-PKG信号通路、唾液分泌、心肌细胞肾上腺素能信号通路、IL-17信号通路、组氨酸代谢、TNF信号通路有关。结论:山茱萸可能通过调节PTGS2、ADRB2、DRD1、ADRA1A等靶点进而干预心源性休克患者的预后。

论苗药血人参药理作用研究进展

在祖国医学的医药宝库中,有中药,苗药,蒙药等,诸多的药物为中华民族的健康保驾护航。苗药是祖国医药的重要组成部分,在云贵地区运用广泛。血人参是临床中常用的苗药之一,在亚洲分布广泛。东亚主要分布在我国,目前主要集中在贵州、广西、湖北、云南等南方地区;东南亚主要分布在泰国、缅甸等国家;南亚主要分布在印度、缅甸等国家。血人参含有丰富的化学成分,目前已知主要有挥发油、甾醇体类、黄烷醇等。血人参在临床中应用较多,根据相关文献总结,血人参具有抗氧化、降糖、降脂、保肝等诸多药理作用。由于血人参为苗药,所以在临床中有些医生对该药的认知尚不深入,因此,笔者查阅国内外相关文献,对血人参的化学成分、药理作用等进行总结,以便为相关科学研究或临床应用提供一定的理论依据和前景价值基础,也为血人参在临床中广泛实用,奠定基础。

百脉根根瘤菌nod-lacZ转录融合子构建及表达分析

根据Mesorhizobium loti基因组信息,克隆nodA、nodB、nodD等基因的启动子序列,分别与报告基因构建相应的转录融合子(nod-lacZ),并分别导入中慢生型百脉根根瘤菌MAFF303099和广谱根瘤菌NGR234(Rhizobium sp.)中,利用百脉根根的抽提物作为诱导物,检测结瘤基因的表达。结果表明:在MAFF303099菌株中,百脉根根抽提物不能诱导nodA的表达;能诱导nodB和nodD1的表达;nodD2呈组成型表达;供试的5种不同的类黄酮化合物都不能诱导nodA基因表达;在NGR234菌株中,毛地黄酮(Luteolin)能诱导nodA表达。

百脉根半胱氨酸蛋白酶基因在根瘤衰老过程中的表达分析

为明确半胱氨酸蛋白酶与定型根瘤衰老的相关性,通过与已鉴定的紫云英半胱氨酸蛋白酶AsNODf32进行序列比对分析,从百脉根中鉴定出2个与根瘤衰老相关的蛋白酶基因LjCyp1和LjCyp5。蛋白序列及结构分析表明,LjCyp1和LjCyp5同源性高达96.2%,均含有2个典型的半胱氨酸蛋白酶结构域和液泡定位信号肽,属于木瓜蛋白酶家族。体外酶活检测显示,2个蛋白都具有半胱氨酸蛋白酶活性。通过Realtime PCR和组织化学定位分析表明,2个蛋白酶基因主要在成熟和衰老的根瘤中表达。结果表明LjCyp1和LjCyp5可能在百脉根根瘤衰老过程中发挥重要作用。

基于网络药理学探讨血人参治疗肺痈的可行性和潜在机制

目的:通过网络药理学和分子对接探讨血人参(Indigofera stachyodes Lindl.)治疗肺痈的可行性和潜在机制。方法:将血人参药材用水煎煮浓缩为1.0 g/ml的药液,大鼠随机分为空白血浆组、给药后1 h采血组、给药后2 h采血组、给药后3 h采血组4组(每组8只),连续灌胃一周,分别采血,制备血浆,运用液质联用技术对四组血浆的化学成分进行鉴定,然后用Venny 2.1作Venn图进行筛选;通过TCMSP、SymMap、SwissTargetPrediction、SEA等数据库筛选血人参成分及靶点。通过GeneCards、OMIM数据库筛选肺痈的疾病靶点。通过R语言获取药物疾病交集靶点,通过蛋白互作和PPI分析获得关键靶点,并利用关键靶点进行KEGG、GO富集分析以及分子对接。结果:通过HPLC-MS分析并排除TCMSP中搜索不到的成分,获得Hypoxanthine等11个血人参活性化合物,337个靶点,2243个肺痈的疾病靶点,“血人参–肺痈”交集靶点113个,关键基因15个,KEGG富集分析结果显示,血人参主要通过调控VEGF信号通路、Rap1信号通路、TNF信号通路等参与肺痈的调节机制。分子对接结果显示,EGFR与Fisetin的亲和力最高;SRC与Fisetin的亲和力最高;PLCG1与2-Naphthylamine的亲和力最高;PIK3R1与Fisetin的亲和力最高;PTK2与Fisetin的亲和力最高。结论:血人参的活性成分可能通过影响免疫反应、炎症反应和细胞凋亡等生物学过程,发挥治疗肺痈的作用。Objective: To explore the feasibility and potential mechanisms of the treatment of lung abscess with Indigofera stachyodes Lindl. through network pharmacology and molecular docking. Methods: The medicinal material of I. stachyodes Lindl. was decocted in water and concentrated into a medicinal solution of 1.0 g/ml. Rats were randomly divided into four groups, including the blank plasma group, the blood collection group at 1 h after administration, the blood collection group at 2 h after administration, and the blood collection group at 3 h after administration (8 rats in each group). The rats were given continuous intragastric administration for one week. Blood samples were collected from each group, and plasma was prepared. The chemical components of the plasma from the four groups were identified by Liquid Chromatography-Mass Spectrometry (LC-MS) and Venn diagrams were performed for screening by Venny 2.1. The chemical components and targets of I. stachyodes Lindl. were screened by TCMSP, SymMap, SwissTargetPrediction, and SEA databases. Disease targets of lung abscess were screened by GeneCards, OMIM database. Drug-disease intersection targets were obtained through R language, key targets were obtained through protein interactions and PPI analysis, and KEGG, GO enrichment analysis and molecular docking were performed using key targets. Results: Through HPLC-MS analysis and exclusion of components that could not be searched in TCMSP, 11 active compounds of I. stachyodes Lindl. such as Hypoxanthine were obtained, along with 337 targets, 2243 disease targets related to lung abscess, and 113 intersection targets between “I. stachyodes Lindl.-lung abscess”. 15 key genes were identified. The results of KEGG enrichment analysis showed that I. stachyodes Lindl. is mainly involved in the regulatory mechanism of lung abscess by regulating VEGF signaling pathway, Rap1 signaling pathway, TNF signaling pathway, etc. Molecular docking results revealed that EGFR had the highest affinity for Fisetin;SRC had the highest affinity for Fisetin;PLCG1 had the highest affinity for 2-Naphthylamine;PIK3R1 had the highest affinity for Fisetin;and PTK2 had the highest affinity for Fisetin. Conclusion: The active ingredients of I. stachyodes Lindl may play a role in the treatment of lung abscess by affecting biological processes such as immune response, inflammatory response, and apoptosis.

基于网络药理学探究鬼针草治疗胃癌的可行性和潜在作用机制

通过网络药理学探讨鬼针草治疗胃癌的可行性和潜在作用机制。方法:利用TCMSP数据库筛选鬼针草的有效活性成分及潜在作用靶点,GeneCards数据库检索胃癌相关基因靶点,使用Venne进行交集对比分析,同时筛选出二者的共同作用靶点。使用Cytoscape软件绘制组分-靶网络图,绘制蛋白互作网络图,将共同靶基因导入DAVID数据库进行GO生物富集及KEGG富集分析。结果:从鬼针草中筛选出6个有效成分,取与胃癌交集基因得到112个,其中关键作用靶点TP53、AKT1、HSP90AA1、JUN等,通过核心靶点富集分析可知,鬼针草对胃癌治疗的信号通路主要是Pathways in cancer,Hepatitis B,Bladder cancer,Prostate cancer和TNF signaling pathway等。结论:鬼针草可能通过活性成分Quercetin、luteolin等调控Pathways in cancer等信号通路上的TP53、AKT1等基因对胃癌进行抗肿瘤活性,为鬼针草的后续研究提供理论依据。