本文利用高效液相色谱 电喷雾 质谱/质谱(HPLC ESI MS/MS)联用对灯盏花提取液进行了分离,并对其中的主要成分进行了定性分析。一级质谱扫描结果表明,灯盏花提取液中主要有4个峰,其中,第一个峰可能为5,6,4 三羟基黄酮 7 o β D 半乳糖醛...本文利用高效液相色谱 电喷雾 质谱/质谱(HPLC ESI MS/MS)联用对灯盏花提取液进行了分离,并对其中的主要成分进行了定性分析。一级质谱扫描结果表明,灯盏花提取液中主要有4个峰,其中,第一个峰可能为5,6,4 三羟基黄酮 7 o β D 半乳糖醛酸苷,第二峰为灯盏花乙素;后两峰为黄芩甙,两者可能互为同分异构体。展开更多
A new on-line coupled supercritical fluid extraction(SFE) with high performance liquid chromatography(HPLC) system was developed.Two three-port valves were used as an interface to connect SFE and HPLC.In this design,t...A new on-line coupled supercritical fluid extraction(SFE) with high performance liquid chromatography(HPLC) system was developed.Two three-port valves were used as an interface to connect SFE and HPLC.In this design,the two important processes including sample preparation and sample analysis were(integrated) into a system.Compared with off-line system,the on-line system has many advantages,such as high sensitivity,short analysis time,avoiding sample pollution,easily operation and ease to automation etc.Its application in the extraction and analysis of triterpenoids to the fruiting bodies of Ganoderma Lucidum was also described.Under the optimized extraction conditions,the similar chromatogram of the extract with on-line SFE- HPLC system was observed as well as that with off-line SFE followed by HPLC.The result showed the great potential of the on-line system in the extraction and analysis of tradition Chinese medicine.展开更多
文摘本文利用高效液相色谱 电喷雾 质谱/质谱(HPLC ESI MS/MS)联用对灯盏花提取液进行了分离,并对其中的主要成分进行了定性分析。一级质谱扫描结果表明,灯盏花提取液中主要有4个峰,其中,第一个峰可能为5,6,4 三羟基黄酮 7 o β D 半乳糖醛酸苷,第二峰为灯盏花乙素;后两峰为黄芩甙,两者可能互为同分异构体。
文摘A new on-line coupled supercritical fluid extraction(SFE) with high performance liquid chromatography(HPLC) system was developed.Two three-port valves were used as an interface to connect SFE and HPLC.In this design,the two important processes including sample preparation and sample analysis were(integrated) into a system.Compared with off-line system,the on-line system has many advantages,such as high sensitivity,short analysis time,avoiding sample pollution,easily operation and ease to automation etc.Its application in the extraction and analysis of triterpenoids to the fruiting bodies of Ganoderma Lucidum was also described.Under the optimized extraction conditions,the similar chromatogram of the extract with on-line SFE- HPLC system was observed as well as that with off-line SFE followed by HPLC.The result showed the great potential of the on-line system in the extraction and analysis of tradition Chinese medicine.
