Objective:To investigate the binding characteri stics of endothelial cell (EC) with LPS free from the participation of serum fac tors. Methods:Laser confocal microscope was employed in the observat ion of the binding ...Objective:To investigate the binding characteri stics of endothelial cell (EC) with LPS free from the participation of serum fac tors. Methods:Laser confocal microscope was employed in the observat ion of the binding of EC with FITC-LPS. The KD and the binding sites of each EC were calculated by radioligand binding assay of receptors (RBA) using [ 3H] -LPS. Results:The binding of EC with LPS was saturable, time and con centration dependent and it could be competed with overdosed LPS of the same typ e. The fluorescence mainly distributed in cytoplasm, especially near the nucleus , which could also be stained. Conclusions:There might be some specific LPS binding sites exi sting on ECs and LPS could function intracellularily.展开更多
文摘Objective:To investigate the binding characteri stics of endothelial cell (EC) with LPS free from the participation of serum fac tors. Methods:Laser confocal microscope was employed in the observat ion of the binding of EC with FITC-LPS. The KD and the binding sites of each EC were calculated by radioligand binding assay of receptors (RBA) using [ 3H] -LPS. Results:The binding of EC with LPS was saturable, time and con centration dependent and it could be competed with overdosed LPS of the same typ e. The fluorescence mainly distributed in cytoplasm, especially near the nucleus , which could also be stained. Conclusions:There might be some specific LPS binding sites exi sting on ECs and LPS could function intracellularily.
