A short ionic liquids （ILs）-based monolithic cartridge was prepared and used as the selective extraction sorbent. After the material was evaluated by field emission-scanning electron microscopy （FE-SEM）, a new app...A short ionic liquids （ILs）-based monolithic cartridge was prepared and used as the selective extraction sorbent. After the material was evaluated by field emission-scanning electron microscopy （FE-SEM）, a new approach for the extraction and determination of quercetin and myricetin from Chamaecyparis obtusa （C. obtusa） by using ILs-based, monolithic cartridge system was developed. Chromatographic analysis was conducted on a C18 column with UV detection at 372 nm, an eluting solution consisting of acetonitrile-water （25/75, V/V） as the mobile phase, and a flow rate of 0.7 mLomin 1. A good linear relationship was demonstrated when the concentrations of quercetin and myricetin were in the range of 0.5--100.0 ggomL-1. The recoveries ranged from 101.6% to 104.6% and the inter- and intra-day relative standard deviations （RSD） were less than 5.0%. This method effectively removed the impurities and avoided tedious pretreatment. It provided a fast, economic and effective method for assaying trace drugs from natural plants.展开更多
基金the National Natural Science Foundation of China(21507062,21475061)the Natural Science of Foundation of Hebei Province(B2018201270)+2 种基金the Scientific Research Foundation for the Returned Overseas Chinese Scholars,State Education Ministry([2015]1098)Program of Natural Science Research of Jiangsu Higher Education Institutions of China(16KJB150022)the Priority Academic Program Development of Jiangsu Higher Education Institutions,Jiangsu Collaborative Innovation Center of Biomedical Functional Materials。
文摘A short ionic liquids （ILs）-based monolithic cartridge was prepared and used as the selective extraction sorbent. After the material was evaluated by field emission-scanning electron microscopy （FE-SEM）, a new approach for the extraction and determination of quercetin and myricetin from Chamaecyparis obtusa （C. obtusa） by using ILs-based, monolithic cartridge system was developed. Chromatographic analysis was conducted on a C18 column with UV detection at 372 nm, an eluting solution consisting of acetonitrile-water （25/75, V/V） as the mobile phase, and a flow rate of 0.7 mLomin 1. A good linear relationship was demonstrated when the concentrations of quercetin and myricetin were in the range of 0.5--100.0 ggomL-1. The recoveries ranged from 101.6% to 104.6% and the inter- and intra-day relative standard deviations （RSD） were less than 5.0%. This method effectively removed the impurities and avoided tedious pretreatment. It provided a fast, economic and effective method for assaying trace drugs from natural plants.
