为探索不同批次活性炭对参芪扶正稀释液中指标成分的吸附规律,本文首先建立了腺嘌呤、腺苷、毛蕊异黄酮苷、黄芪甲苷等四种指标成分含量的HPLC检测方法,并进行了方法学考察。结果表明腺嘌呤、腺苷、毛蕊异黄酮苷、黄芪甲苷分别在2.5~15 ...为探索不同批次活性炭对参芪扶正稀释液中指标成分的吸附规律,本文首先建立了腺嘌呤、腺苷、毛蕊异黄酮苷、黄芪甲苷等四种指标成分含量的HPLC检测方法,并进行了方法学考察。结果表明腺嘌呤、腺苷、毛蕊异黄酮苷、黄芪甲苷分别在2.5~15 µg/mL、5~30 µg/mL、5~30 µg/mL、0.1~0.8 mg/mL范围内线性关系良好。吸附动力学实验研究结果表明,不同批次活性炭吸附速率及平衡吸附量均存在较大差异。另外采用拟一级动力学和拟二级动力学对活性炭吸附过程进行建模,发现拟二级动力学能更好地拟合活性炭吸附过程。 To explore the adsorption law of different batches of activated carbon on the indicative constituents in the dilution of Shenqi Fuzheng Diluent, HPLC method for the detection of the content of four indicative constituents, including adenine, adenosine, calycosin 7-o-glucoside, and astragaloside A, was established, and the methodology was evaluated. The results showed that adenine, adenosine, calycosin 7-o-glucoside, and astragaloside A had a good linear relationship in the ranges of 2.5~15 μg/mL, 5~30 μg/mL, 5~30 μg/mL and 0.1~0.8 mg/mL, respectively. The results of adsorption kinetic experiments showed that there were great differences in the adsorption rate and equilibrium adsorption amount of different batches of activated carbon. In addition, the activated carbon adsorption process was modeled by pseudo-first-order kinetics and quasi-second-order kinetics, and it was found that the quasi-second-order kinetics could better fit the activated carbon adsorption process.展开更多
文摘为探索不同批次活性炭对参芪扶正稀释液中指标成分的吸附规律,本文首先建立了腺嘌呤、腺苷、毛蕊异黄酮苷、黄芪甲苷等四种指标成分含量的HPLC检测方法,并进行了方法学考察。结果表明腺嘌呤、腺苷、毛蕊异黄酮苷、黄芪甲苷分别在2.5~15 µg/mL、5~30 µg/mL、5~30 µg/mL、0.1~0.8 mg/mL范围内线性关系良好。吸附动力学实验研究结果表明,不同批次活性炭吸附速率及平衡吸附量均存在较大差异。另外采用拟一级动力学和拟二级动力学对活性炭吸附过程进行建模,发现拟二级动力学能更好地拟合活性炭吸附过程。 To explore the adsorption law of different batches of activated carbon on the indicative constituents in the dilution of Shenqi Fuzheng Diluent, HPLC method for the detection of the content of four indicative constituents, including adenine, adenosine, calycosin 7-o-glucoside, and astragaloside A, was established, and the methodology was evaluated. The results showed that adenine, adenosine, calycosin 7-o-glucoside, and astragaloside A had a good linear relationship in the ranges of 2.5~15 μg/mL, 5~30 μg/mL, 5~30 μg/mL and 0.1~0.8 mg/mL, respectively. The results of adsorption kinetic experiments showed that there were great differences in the adsorption rate and equilibrium adsorption amount of different batches of activated carbon. In addition, the activated carbon adsorption process was modeled by pseudo-first-order kinetics and quasi-second-order kinetics, and it was found that the quasi-second-order kinetics could better fit the activated carbon adsorption process.
