目的:探讨NR5A2 rs3790843基因多态性与女性胃癌患者临床病理特征及预后的关系。方法:检测211例女性胃癌患者石蜡组织DNA上rs3790843基因多态性。采用Kaplan-Meier法、Log-rank检验及Cox回归分析NR5A2 rs3790843基因多态性对女性胃癌患...目的:探讨NR5A2 rs3790843基因多态性与女性胃癌患者临床病理特征及预后的关系。方法:检测211例女性胃癌患者石蜡组织DNA上rs3790843基因多态性。采用Kaplan-Meier法、Log-rank检验及Cox回归分析NR5A2 rs3790843基因多态性对女性胃癌患者临床病理特征及预后的影响。结果:非贲门癌组较贲门癌组能显著降低死亡风险,而有淋巴转移、远处转移、TNM分期较晚的患者,其死亡风险明显提高。在共显性模型中(TT vs TC vs CC),携带TC基因型的患者相较与携带TT基因的患者,死亡风险明显降低(HR=0.616,95%CI 0.409~0.928),在显性基因模型中(TT v s TC/CC),携带TC/CC基因型的胃癌患者死亡风险相较携带T T基因型者,死亡风险明显降低(HR=0.619,95%CI 0.417~0.916,P=0.015)。而在隐性模型中(TT/TC vs CC),携带TT/TC基因型的患者与携带CC基因型的患者的生存无显著差异。分层分析发现:在年龄≤60岁、非贲门癌、肿瘤>5 cm、有淋巴结转移、无远处转移、弥漫性胃癌、分化程度差、无术后辅助化疗、T4组、临床分期III期的分组中,rs3790843基因多态性能显著降低死亡风险。多因素Cox回归分析结果表明:有无淋巴结转移、肿瘤部位、rs3790843(TT vs TC/CC)均可作为影响女性胃癌患者的独立预后因子。结论:NR5A2 rs3790843基因的T→C的突变能显著降低女性胃癌患者的死亡风险,是一个女性胃癌预后的保护性因素,可作为女性胃癌患者预后的独立预测指标。展开更多
To examine the ability of intrastriatal gene transfer of vascular endothelial growth factor 165 mediated by adenoviral vector to rescue dopaminergic neurons in a rat model of Parkinson＇s disease （PD）, we constructe...To examine the ability of intrastriatal gene transfer of vascular endothelial growth factor 165 mediated by adenoviral vector to rescue dopaminergic neurons in a rat model of Parkinson＇s disease （PD）, we constructed recombinant replication-deficent adenoviral vectors carrying the gene of VEGF165 （Ad-VEGF）, and injected Ad-VEGF （or Ad-LacZ and PBS as controls） into the striatum of rats 7 days after the lesion by 6-hydroxydopamine. The rat rotational behavior analysis and tyrosine hydroxylase （TH） immunohistochemistry were performed to assess the change of dopaminergic neurons. Our results showed that the rats receiving Ad-VEGF injection displayed a significant improvement in apomorphine-induced rotational behavior and a significant preservation of TH-positive neurons and fibers compared with control animals, It is concluded that intrastriatal gene transfer by Ad-VEGF may rescue the dopaminergic neurons from degeneration in a rat model of PD.展开更多
文摘目的:探讨NR5A2 rs3790843基因多态性与女性胃癌患者临床病理特征及预后的关系。方法:检测211例女性胃癌患者石蜡组织DNA上rs3790843基因多态性。采用Kaplan-Meier法、Log-rank检验及Cox回归分析NR5A2 rs3790843基因多态性对女性胃癌患者临床病理特征及预后的影响。结果:非贲门癌组较贲门癌组能显著降低死亡风险,而有淋巴转移、远处转移、TNM分期较晚的患者,其死亡风险明显提高。在共显性模型中(TT vs TC vs CC),携带TC基因型的患者相较与携带TT基因的患者,死亡风险明显降低(HR=0.616,95%CI 0.409~0.928),在显性基因模型中(TT v s TC/CC),携带TC/CC基因型的胃癌患者死亡风险相较携带T T基因型者,死亡风险明显降低(HR=0.619,95%CI 0.417~0.916,P=0.015)。而在隐性模型中(TT/TC vs CC),携带TT/TC基因型的患者与携带CC基因型的患者的生存无显著差异。分层分析发现:在年龄≤60岁、非贲门癌、肿瘤>5 cm、有淋巴结转移、无远处转移、弥漫性胃癌、分化程度差、无术后辅助化疗、T4组、临床分期III期的分组中,rs3790843基因多态性能显著降低死亡风险。多因素Cox回归分析结果表明:有无淋巴结转移、肿瘤部位、rs3790843(TT vs TC/CC)均可作为影响女性胃癌患者的独立预后因子。结论:NR5A2 rs3790843基因的T→C的突变能显著降低女性胃癌患者的死亡风险,是一个女性胃癌预后的保护性因素,可作为女性胃癌患者预后的独立预测指标。
文摘To examine the ability of intrastriatal gene transfer of vascular endothelial growth factor 165 mediated by adenoviral vector to rescue dopaminergic neurons in a rat model of Parkinson＇s disease （PD）, we constructed recombinant replication-deficent adenoviral vectors carrying the gene of VEGF165 （Ad-VEGF）, and injected Ad-VEGF （or Ad-LacZ and PBS as controls） into the striatum of rats 7 days after the lesion by 6-hydroxydopamine. The rat rotational behavior analysis and tyrosine hydroxylase （TH） immunohistochemistry were performed to assess the change of dopaminergic neurons. Our results showed that the rats receiving Ad-VEGF injection displayed a significant improvement in apomorphine-induced rotational behavior and a significant preservation of TH-positive neurons and fibers compared with control animals, It is concluded that intrastriatal gene transfer by Ad-VEGF may rescue the dopaminergic neurons from degeneration in a rat model of PD.
