The cultures of Platymonas subcordiformis and Isochrysis zhangjiangensis which had preserved for long time was irradiated by Nd:YAG laser(1.06 μm,5 W)with different dose.Then,the effects of laser on algae growth,phys...The cultures of Platymonas subcordiformis and Isochrysis zhangjiangensis which had preserved for long time was irradiated by Nd:YAG laser(1.06 μm,5 W)with different dose.Then,the effects of laser on algae growth,physiological and biochemical characteristic were investigated.The feasibility to utilize laser in the activation and rejuvenation of microalgae were discussed.The results showed that the optimal dosage to rejuvenate I.zhangjiangensis is 60 s,which the growth rate,cell biomass,the contents of chlorophyll,polysaccharides and protein increased by 27.59%-43.44% after Nd:YAG radiation.For P.subcordiformis,the better results of rejuvenation by Nd:YAG laser was got when irradiation dose at both 60 s and 90 s.It is favourable to the growth of P.subcordiformis by using lower irradiation dose of Nd:YAG laser,which the growth rate and the contents of chlorophyll increased by 12.5% and 17.5% respectively.By contrast,the higher irradiation dose was favourable to the increase of other biomass of P.subcordiformis,which the contents of carotenoids,protein and polysaccharides increased by 87.7%,15.5% and 36.8% respectively.The results of Laser Scanning Confocal Microscope(LSCM)showed that the fluorescence spectra of the irradiated group is similar to control group with the maximum peak located at 682 nm which can be attributed to photosystemⅡ.While the irradiated group which the growth had be promoted has a higher intensity at 682 nm than control group.In this paper,the possible mechanism of activation and rejuvenation effects on microalgae induced by laser irradiation was also discussed.These results can offer a theoretical foundation to utilize laser in breeding of microalgae species for aquiculture.展开更多
梅花山自然保护区,位于闽西上杭,连城和龙岩三县之间,其邻近县有长汀、武平和永定三个县。我们通过先后调查26种脊椎动物(鱼类4种、两栖类7种、爬行类4种、鸟类3种、哺乳类8种),共检得蠕虫116种,分隶于60科84属(吸虫18科29属40种;绦虫7...梅花山自然保护区,位于闽西上杭,连城和龙岩三县之间,其邻近县有长汀、武平和永定三个县。我们通过先后调查26种脊椎动物(鱼类4种、两栖类7种、爬行类4种、鸟类3种、哺乳类8种),共检得蠕虫116种,分隶于60科84属(吸虫18科29属40种;绦虫7科8属10种;线虫31科43属61种;棘头虫4科4属5种),其中3种是新种:蛙后狭盲肠线虫Metangusticaecum ranae Wang et Wang,1989、福建后狭盲线虫M.fujianensis Wang,sp.nov.和少栉副弱尾线虫Paracosmocerca oligolektana Wang sp.nov.展开更多
文摘The cultures of Platymonas subcordiformis and Isochrysis zhangjiangensis which had preserved for long time was irradiated by Nd:YAG laser(1.06 μm,5 W)with different dose.Then,the effects of laser on algae growth,physiological and biochemical characteristic were investigated.The feasibility to utilize laser in the activation and rejuvenation of microalgae were discussed.The results showed that the optimal dosage to rejuvenate I.zhangjiangensis is 60 s,which the growth rate,cell biomass,the contents of chlorophyll,polysaccharides and protein increased by 27.59%-43.44% after Nd:YAG radiation.For P.subcordiformis,the better results of rejuvenation by Nd:YAG laser was got when irradiation dose at both 60 s and 90 s.It is favourable to the growth of P.subcordiformis by using lower irradiation dose of Nd:YAG laser,which the growth rate and the contents of chlorophyll increased by 12.5% and 17.5% respectively.By contrast,the higher irradiation dose was favourable to the increase of other biomass of P.subcordiformis,which the contents of carotenoids,protein and polysaccharides increased by 87.7%,15.5% and 36.8% respectively.The results of Laser Scanning Confocal Microscope(LSCM)showed that the fluorescence spectra of the irradiated group is similar to control group with the maximum peak located at 682 nm which can be attributed to photosystemⅡ.While the irradiated group which the growth had be promoted has a higher intensity at 682 nm than control group.In this paper,the possible mechanism of activation and rejuvenation effects on microalgae induced by laser irradiation was also discussed.These results can offer a theoretical foundation to utilize laser in breeding of microalgae species for aquiculture.
文摘梅花山自然保护区,位于闽西上杭,连城和龙岩三县之间,其邻近县有长汀、武平和永定三个县。我们通过先后调查26种脊椎动物(鱼类4种、两栖类7种、爬行类4种、鸟类3种、哺乳类8种),共检得蠕虫116种,分隶于60科84属(吸虫18科29属40种;绦虫7科8属10种;线虫31科43属61种;棘头虫4科4属5种),其中3种是新种:蛙后狭盲肠线虫Metangusticaecum ranae Wang et Wang,1989、福建后狭盲线虫M.fujianensis Wang,sp.nov.和少栉副弱尾线虫Paracosmocerca oligolektana Wang sp.nov.
