[Objective] The aim was to provide molecular basis for the identification of species in the moss family Bryaceae by the construction of inter-simple sequence repeats （ISSR） fingerprinting. [Method] In order to seek ...[Objective] The aim was to provide molecular basis for the identification of species in the moss family Bryaceae by the construction of inter-simple sequence repeats （ISSR） fingerprinting. [Method] In order to seek standardizing PCR reaction set-up, an orthogonal design was used to optimize ISSR-PCR amplification system of Bryaceae in five factors （Mg2＋, dNTPs, primer, DNA template, Taq DNA polymerase） at four levels respectively. [Result] A suitable ISSR reaction system was obtained, namely： 20 μl reaction system containing 5 ng of DNA template, 0.2 μmol/L primer, 2.25 mmol/L MgCl2, 0.6 U of Taq DNA polymerase, 0.4 mmol/L dNTPs. Proper annealing temperature was found at 48-50 ℃.The above system and six ISSR-PCR primers were used for the PCR amplification of 14 samples from Bryaceae and the related species in Mniaceae. A total of 86 bands were amplified, all showed polymorphism. NJ cluster analysis showed a star-shaped cladogram. [Conclusion] The results manifested that ISSR fingerprinting could provide the appropriate degree of polymorphism at low taxonomic level, so it would be a useful tool to provide additional evidence for resolving taxonomic relationships at the species level of Bryaceae.展开更多
[Objective] This study aimed to clarify the effects of Pb2＋ and Cr3＋ stress on the contents of chlorophyl in Bryum argenteum and Barbula indica. [Method] B. ar-genteum and B. indica were cultured in different concen...[Objective] This study aimed to clarify the effects of Pb2＋ and Cr3＋ stress on the contents of chlorophyl in Bryum argenteum and Barbula indica. [Method] B. ar-genteum and B. indica were cultured in different concentrations of lead nitrate solu-tion or chromium chloride solution, to investigate the changes in contents of photo-synthetic pigments under lead and chromium single stress. [Result] The results showed that the effects of 1, 10 and 200 mg/L Pb2＋ on B. indica were greater than those on B. argenteum; the effects of 1, 50, 100 and 200 mg/L Cr3＋ on B. argen-teum were greater than those on B. indica. Total chlorophyl content in B. argen-teum and B. indica under different concentrations of Pb2＋ and Cr3＋ was decreased compared with that in control. The tolerance of B. indica to Pb2＋ pol ution was lower than that of B. argenteum, while the tolerance of B. indica to Cr3＋ pol ution was greater than that of B. argenteum. [Conclusion] Based on the conventional require-ments for monitoring materials, B. indica can be used as an indicator species to monitor heavy metal pol ution in the atmosphere of Zhengzhou.展开更多
基金Supported by Natural Science Foundation of Hebei Province(C2006000147)Zhengzhou Science and Technology Program(10PTGN449-6)~~
文摘[Objective] The aim was to provide molecular basis for the identification of species in the moss family Bryaceae by the construction of inter-simple sequence repeats （ISSR） fingerprinting. [Method] In order to seek standardizing PCR reaction set-up, an orthogonal design was used to optimize ISSR-PCR amplification system of Bryaceae in five factors （Mg2＋, dNTPs, primer, DNA template, Taq DNA polymerase） at four levels respectively. [Result] A suitable ISSR reaction system was obtained, namely： 20 μl reaction system containing 5 ng of DNA template, 0.2 μmol/L primer, 2.25 mmol/L MgCl2, 0.6 U of Taq DNA polymerase, 0.4 mmol/L dNTPs. Proper annealing temperature was found at 48-50 ℃.The above system and six ISSR-PCR primers were used for the PCR amplification of 14 samples from Bryaceae and the related species in Mniaceae. A total of 86 bands were amplified, all showed polymorphism. NJ cluster analysis showed a star-shaped cladogram. [Conclusion] The results manifested that ISSR fingerprinting could provide the appropriate degree of polymorphism at low taxonomic level, so it would be a useful tool to provide additional evidence for resolving taxonomic relationships at the species level of Bryaceae.
基金Supported by Science and Technology Supporting Project of Henan Province(132300410358)~~
文摘[Objective] This study aimed to clarify the effects of Pb2＋ and Cr3＋ stress on the contents of chlorophyl in Bryum argenteum and Barbula indica. [Method] B. ar-genteum and B. indica were cultured in different concentrations of lead nitrate solu-tion or chromium chloride solution, to investigate the changes in contents of photo-synthetic pigments under lead and chromium single stress. [Result] The results showed that the effects of 1, 10 and 200 mg/L Pb2＋ on B. indica were greater than those on B. argenteum; the effects of 1, 50, 100 and 200 mg/L Cr3＋ on B. argen-teum were greater than those on B. indica. Total chlorophyl content in B. argen-teum and B. indica under different concentrations of Pb2＋ and Cr3＋ was decreased compared with that in control. The tolerance of B. indica to Pb2＋ pol ution was lower than that of B. argenteum, while the tolerance of B. indica to Cr3＋ pol ution was greater than that of B. argenteum. [Conclusion] Based on the conventional require-ments for monitoring materials, B. indica can be used as an indicator species to monitor heavy metal pol ution in the atmosphere of Zhengzhou.
