Aim:To study the antiatherosclerosis mechanism of scallop skirt glycosaminoglycan(SS-GAG)by observing the effect of SS-GAG on the oxidation of low density lipoprotein in macrophage and on the activities of glutathion ...Aim:To study the antiatherosclerosis mechanism of scallop skirt glycosaminoglycan(SS-GAG)by observing the effect of SS-GAG on the oxidation of low density lipoprotein in macrophage and on the activities of glutathion peroxidase(GSH-PX)and myeloperoxidase(MPO).Methods:The cell line was established by in vitro culturing mouse macrophage RAW264.7 was employed to study the effects of SS-GAG on the oxidation of LDL and the activities of relevant enzymes in macrophage via the assay of concentrations of malonaldehyde(MDA),conjugated diene,activity of GSH-PX,and MPO.Results:After macrophage incubation with LDL for 24 h,we found the enhanced activity of MPO,decreased activity of GSH-PX,and increased production of lipid peroxidation.Moreover,SS-GAG could increase the activity of GSH-PX but attenuate the activity of MPO.The decreased production of lipid peroxidation were also observed.There also existed dose-effect relationship in our findings.Conclusion:SS-GAG could depress the oxidation of LDL by degrading the activity of MPO and increasing the activity of GSH-PX.This may be one of its mechanisms of antiatherosclerosis action.展开更多
目的探讨早发冠心病患者的危险因素、临床症状和冠状动脉病变特点的性别差异。方法连续入选我科2009年5月-2014年10月经冠脉造影确诊的198例早发冠心病患者,分为男性组(年龄〈55岁,n=135)和女性组(年龄〈65岁,n=63)。观察两组在冠...目的探讨早发冠心病患者的危险因素、临床症状和冠状动脉病变特点的性别差异。方法连续入选我科2009年5月-2014年10月经冠脉造影确诊的198例早发冠心病患者,分为男性组(年龄〈55岁,n=135)和女性组(年龄〈65岁,n=63)。观察两组在冠心病危险因素、临床症状和冠脉造影特点等方面的差异,探讨早发冠心病患者的性别特征。结果男性组吸烟(83% vs. 5%,P〈0.01)、肥胖(28% vs. 16%,P〈0.05)和饮酒的比例(39% vs. 2%,P〈0.01)显著高于女性组,而女性组高血压病患者的比例高于男性组(70% vs. 51%,P〈0.05);两组糖尿病、血脂异常患者的比例之间差别无统计学意义。男性组血清三酰甘油水平较女性组高〔(2.0±1.4) mmol/L vs.(1.6±0.9) mmol/L,P〈0.05〕,而高密度脂蛋白胆固醇〔(1.03±0.23) mmol/L vs.(1.18±0.27) mmol/L,P〈0.01〕和载脂蛋白A水平〔(1.11±0.19) g/L vs.(1.20±0.23) g/L,P〈0.01〕和载脂蛋白A/B比值〔(1.4±0.5) vs.(1.6±0.5),P〈0.01〕则均较女性组低。男性组危险因素聚集较女性组明显〔(1.9±1.0) vs.( 1.1±0.8),P〈0.01〕;职业分布中,工人在两组所占比例均较高(50% vs. 40%),但男女组间无显著差异。与男性组比较,女性下岗或无业比例较更高(12% vs. 30%,P〈0.01);两组无胸痛比例(35% vs. 46 %)无显著差异,同时,男性患者出汗较为常见(44% vs. 29%,P〈0.05),而女性组心悸现象则较多见(7% vs. 22%,P〈0.01)。两组冠状动脉造影结果显示均以单支病变为主(49% vs. 60%),病变血管均以前降支最为多见(68% vs. 54%)。两组冠状动脉造影结果比较,病变血管数、罪犯血管分布和累及植入支架比例之间差别均无统计学意义。结论早发冠心病患者临床特征存在性别差异,但冠状动脉病变分布特征二者之间无显著差别。展开更多
文摘Aim:To study the antiatherosclerosis mechanism of scallop skirt glycosaminoglycan(SS-GAG)by observing the effect of SS-GAG on the oxidation of low density lipoprotein in macrophage and on the activities of glutathion peroxidase(GSH-PX)and myeloperoxidase(MPO).Methods:The cell line was established by in vitro culturing mouse macrophage RAW264.7 was employed to study the effects of SS-GAG on the oxidation of LDL and the activities of relevant enzymes in macrophage via the assay of concentrations of malonaldehyde(MDA),conjugated diene,activity of GSH-PX,and MPO.Results:After macrophage incubation with LDL for 24 h,we found the enhanced activity of MPO,decreased activity of GSH-PX,and increased production of lipid peroxidation.Moreover,SS-GAG could increase the activity of GSH-PX but attenuate the activity of MPO.The decreased production of lipid peroxidation were also observed.There also existed dose-effect relationship in our findings.Conclusion:SS-GAG could depress the oxidation of LDL by degrading the activity of MPO and increasing the activity of GSH-PX.This may be one of its mechanisms of antiatherosclerosis action.
文摘目的探讨早发冠心病患者的危险因素、临床症状和冠状动脉病变特点的性别差异。方法连续入选我科2009年5月-2014年10月经冠脉造影确诊的198例早发冠心病患者,分为男性组(年龄〈55岁,n=135)和女性组(年龄〈65岁,n=63)。观察两组在冠心病危险因素、临床症状和冠脉造影特点等方面的差异,探讨早发冠心病患者的性别特征。结果男性组吸烟(83% vs. 5%,P〈0.01)、肥胖(28% vs. 16%,P〈0.05)和饮酒的比例(39% vs. 2%,P〈0.01)显著高于女性组,而女性组高血压病患者的比例高于男性组(70% vs. 51%,P〈0.05);两组糖尿病、血脂异常患者的比例之间差别无统计学意义。男性组血清三酰甘油水平较女性组高〔(2.0±1.4) mmol/L vs.(1.6±0.9) mmol/L,P〈0.05〕,而高密度脂蛋白胆固醇〔(1.03±0.23) mmol/L vs.(1.18±0.27) mmol/L,P〈0.01〕和载脂蛋白A水平〔(1.11±0.19) g/L vs.(1.20±0.23) g/L,P〈0.01〕和载脂蛋白A/B比值〔(1.4±0.5) vs.(1.6±0.5),P〈0.01〕则均较女性组低。男性组危险因素聚集较女性组明显〔(1.9±1.0) vs.( 1.1±0.8),P〈0.01〕;职业分布中,工人在两组所占比例均较高(50% vs. 40%),但男女组间无显著差异。与男性组比较,女性下岗或无业比例较更高(12% vs. 30%,P〈0.01);两组无胸痛比例(35% vs. 46 %)无显著差异,同时,男性患者出汗较为常见(44% vs. 29%,P〈0.05),而女性组心悸现象则较多见(7% vs. 22%,P〈0.01)。两组冠状动脉造影结果显示均以单支病变为主(49% vs. 60%),病变血管均以前降支最为多见(68% vs. 54%)。两组冠状动脉造影结果比较,病变血管数、罪犯血管分布和累及植入支架比例之间差别均无统计学意义。结论早发冠心病患者临床特征存在性别差异,但冠状动脉病变分布特征二者之间无显著差别。