应用抗人单核细胞单克隆抗体 Leu3a,Leu5b,Leu2b,LeuM1,Leu9,Leu10,CD21,对54例胃癌和9例胃良性病变的胃局部引流淋巴结进行 ABC 免疫组化染色,原位观察淋巴结中单核细胞分布状况。胃癌局部引流淋巴结 B 区和 T 区有不同程度的增生,前...应用抗人单核细胞单克隆抗体 Leu3a,Leu5b,Leu2b,LeuM1,Leu9,Leu10,CD21,对54例胃癌和9例胃良性病变的胃局部引流淋巴结进行 ABC 免疫组化染色,原位观察淋巴结中单核细胞分布状况。胃癌局部引流淋巴结 B 区和 T 区有不同程度的增生,前者更明显。淋巴滤泡以第Ⅱ～Ⅲ期为主;T 小结以散在高内皮静脉和指突状网状细胞、T 辅助细胞聚集的第Ⅱ期 T 小结为多;区中激活 T数量较良性病变淋巴结中激活 T 数量少;转移灶中抑制 T 淋巴细胞数量增加,裸细胞数量无明显增加。展开更多
Monoclonal antibody (MAb) to rat liver cyto-chrome P-450j isozyme, an activating enzyme specific to nitrosamine metabolism, was used coupled with immunoblotting, densitometer scanning of SDS-PAGE gels and immunohistoc...Monoclonal antibody (MAb) to rat liver cyto-chrome P-450j isozyme, an activating enzyme specific to nitrosamine metabolism, was used coupled with immunoblotting, densitometer scanning of SDS-PAGE gels and immunohistochemical technique. The trace P-450HSj isozyme (Mr. 51.5 Kd) was found in human gastric mucosa. It was similar to P-450j in molecular weight, catalytic and immunochemical properties. The concentrations of P-450HSj in mucosa of lesser curvature were higher than those in greater curvature. This might be one of the important reasons that lesser curvature is the commonest area for gastric carcinoma. But there was possibly less P-450HSj in gastric mucosa with cancer. Im-munohistochemically, P-450HSj was discovered in the cytoplasm of some glandular epithelial cells, especially in the glands with hyperplastic and intestinal metaplastic changes adjacent to carcinoma. It was also found in some normal glands and in tumor cells of high-differentiated adenocarcinoma, but not in those of low-differentiated ones. Following subjects are discussed: (1) the method of detecting trace P-450HSj, (2) the rule of distribution of P-450HSj, and (3) the relationship between the isozyme and the occurrence of gastric cancer caused by nitrosa-mines.展开更多
文摘应用抗人单核细胞单克隆抗体 Leu3a,Leu5b,Leu2b,LeuM1,Leu9,Leu10,CD21,对54例胃癌和9例胃良性病变的胃局部引流淋巴结进行 ABC 免疫组化染色,原位观察淋巴结中单核细胞分布状况。胃癌局部引流淋巴结 B 区和 T 区有不同程度的增生,前者更明显。淋巴滤泡以第Ⅱ～Ⅲ期为主;T 小结以散在高内皮静脉和指突状网状细胞、T 辅助细胞聚集的第Ⅱ期 T 小结为多;区中激活 T数量较良性病变淋巴结中激活 T 数量少;转移灶中抑制 T 淋巴细胞数量增加,裸细胞数量无明显增加。
文摘Monoclonal antibody (MAb) to rat liver cyto-chrome P-450j isozyme, an activating enzyme specific to nitrosamine metabolism, was used coupled with immunoblotting, densitometer scanning of SDS-PAGE gels and immunohistochemical technique. The trace P-450HSj isozyme (Mr. 51.5 Kd) was found in human gastric mucosa. It was similar to P-450j in molecular weight, catalytic and immunochemical properties. The concentrations of P-450HSj in mucosa of lesser curvature were higher than those in greater curvature. This might be one of the important reasons that lesser curvature is the commonest area for gastric carcinoma. But there was possibly less P-450HSj in gastric mucosa with cancer. Im-munohistochemically, P-450HSj was discovered in the cytoplasm of some glandular epithelial cells, especially in the glands with hyperplastic and intestinal metaplastic changes adjacent to carcinoma. It was also found in some normal glands and in tumor cells of high-differentiated adenocarcinoma, but not in those of low-differentiated ones. Following subjects are discussed: (1) the method of detecting trace P-450HSj, (2) the rule of distribution of P-450HSj, and (3) the relationship between the isozyme and the occurrence of gastric cancer caused by nitrosa-mines.
