Enriched-aromatics oils,surfactants and a new activator were used as the additi ves. The effects of intensifying distillation on Daqing crude oil were determine d at different concentrations and distillation temperatu...Enriched-aromatics oils,surfactants and a new activator were used as the additi ves. The effects of intensifying distillation on Daqing crude oil were determine d at different concentrations and distillation temperatures by simple distillati on and TBP distillation. The mechanisms of intensifying distillation of crude oi l by using different additives are discussed.The experimental results show that the types of additives, concentrations and distillation temperatures are main fa ctors which affect intensifying distillation of crude oil.The intensifying effe ct of the new activator developed in this work is better than that of the other additives.展开更多
目的探讨趋化因子(CXCL)-8对高糖诱导人间充质干细胞(MSC)损伤的保护作用及机制。方法建立高糖(含30 mmol/L葡萄糖)培养模型;pcD NA3.1质粒转染MSC为CXCL-8-MSC组,仅转染pcD NA 3.1质粒者为pcD NA 3.1组,在CXCL-8-MSC组中添加Triciribin...目的探讨趋化因子(CXCL)-8对高糖诱导人间充质干细胞(MSC)损伤的保护作用及机制。方法建立高糖(含30 mmol/L葡萄糖)培养模型;pcD NA3.1质粒转染MSC为CXCL-8-MSC组,仅转染pcD NA 3.1质粒者为pcD NA 3.1组,在CXCL-8-MSC组中添加Triciribine为Akt抑制剂组。高糖培养条件下,利用CCK8细胞增殖实验、Western印迹或酶联免疫吸附法(ELISA)分别检测各组MSC增殖的光密度值或Caspase-3、Akt、STAT3和血管内皮生长因子(VEGF)等蛋白的表达。结果高糖环境下,与pcD NA3.1组相比,CXCL-8-MSC组MSC增殖OD值明显升高,Caspase-3蛋白表达明显下降(P<0.01);CXCL-8-MSC组Akt、STAT3和VEGF蛋白含量明显高于pcD NA3.1组(P<0.01);但与CXCL-8-MSC组相比,Akt抑制剂组MSC增殖的OD值明显降低,Caspase-3蛋白表达明显升高(P<0.01);相关蛋白含量均明显降低(P<0.01)。结论 CXCL-8在高糖环境下,通过Akt-STAT3通路,促进MSC旁分泌VEGF等细胞因子,发挥对MSC的保护作用,对保护移植的MSC对抗糖尿病性损伤具有重要作用。展开更多
By using activator to change the state of crude oil and to diminish the size of dispersed phase,mass transfer in distillation process is intensified and distillate yield is increased.In this work,the new activators R1...By using activator to change the state of crude oil and to diminish the size of dispersed phase,mass transfer in distillation process is intensified and distillate yield is increased.In this work,the new activators R1 and R2 were used as the additives respectively,and the intensifying effects to Daqing crude oil were examined at different concentrations and liquid phase temperatures by means of simple distillation and TBP distillation.The interaction mechanisms between the new activators and the crude oil or residues were analyzed,the intensifying effects by the new activators were also studied.Finally,the conceptual technique of “chemical intensification distillation”was put forward.展开更多
目的探讨白细胞介素8(IL-8)对高糖诱导人脂肪间充质干细胞(h Ad MSC)损伤的保护作用。方法含250 mmol/L葡萄糖的培养基建立细胞高糖模型;P65质粒转染人IL-8基因到h Ad MSC为IL-8转染组,仅转染P65质粒者为P65对照组,在IL-8转染组中添加PD...目的探讨白细胞介素8(IL-8)对高糖诱导人脂肪间充质干细胞(h Ad MSC)损伤的保护作用。方法含250 mmol/L葡萄糖的培养基建立细胞高糖模型;P65质粒转染人IL-8基因到h Ad MSC为IL-8转染组,仅转染P65质粒者为P65对照组,在IL-8转染组中添加PD98059为Erk抑制剂组。利用MTT细胞增殖实验、ELISA或流式细胞技术检测各组h Ad MSC增殖的光密度值(OD值)、Caspase-3、Erk或VEGF等蛋白的表达。结果与P65对照组比较,IL-8转染组h Ad MSC增殖OD值明显升高,Caspase-3蛋白表达下降(P<0.01);IL-8转染组Erk蛋白活性和VEGF蛋白含量明显高于P65对照组(P<0.01);但与IL-8转染组比较,Erk抑制剂组h Ad MSC增殖的OD值降低,Caspase-3蛋白表达升高(P<0.01)。结论 IL-8在高糖环境下,通过Erk通路,发挥对h Ad MSC的保护作用。展开更多
基金美国IET教育基金资助项目及石油大学重质油加工国家重点实验室开放基金部分资助项目 (No 2 0 0 0 15 )
文摘Enriched-aromatics oils,surfactants and a new activator were used as the additi ves. The effects of intensifying distillation on Daqing crude oil were determine d at different concentrations and distillation temperatures by simple distillati on and TBP distillation. The mechanisms of intensifying distillation of crude oi l by using different additives are discussed.The experimental results show that the types of additives, concentrations and distillation temperatures are main fa ctors which affect intensifying distillation of crude oil.The intensifying effe ct of the new activator developed in this work is better than that of the other additives.
文摘目的探讨趋化因子(CXCL)-8对高糖诱导人间充质干细胞(MSC)损伤的保护作用及机制。方法建立高糖(含30 mmol/L葡萄糖)培养模型;pcD NA3.1质粒转染MSC为CXCL-8-MSC组,仅转染pcD NA 3.1质粒者为pcD NA 3.1组,在CXCL-8-MSC组中添加Triciribine为Akt抑制剂组。高糖培养条件下,利用CCK8细胞增殖实验、Western印迹或酶联免疫吸附法(ELISA)分别检测各组MSC增殖的光密度值或Caspase-3、Akt、STAT3和血管内皮生长因子(VEGF)等蛋白的表达。结果高糖环境下,与pcD NA3.1组相比,CXCL-8-MSC组MSC增殖OD值明显升高,Caspase-3蛋白表达明显下降(P<0.01);CXCL-8-MSC组Akt、STAT3和VEGF蛋白含量明显高于pcD NA3.1组(P<0.01);但与CXCL-8-MSC组相比,Akt抑制剂组MSC增殖的OD值明显降低,Caspase-3蛋白表达明显升高(P<0.01);相关蛋白含量均明显降低(P<0.01)。结论 CXCL-8在高糖环境下,通过Akt-STAT3通路,促进MSC旁分泌VEGF等细胞因子,发挥对MSC的保护作用,对保护移植的MSC对抗糖尿病性损伤具有重要作用。
文摘By using activator to change the state of crude oil and to diminish the size of dispersed phase,mass transfer in distillation process is intensified and distillate yield is increased.In this work,the new activators R1 and R2 were used as the additives respectively,and the intensifying effects to Daqing crude oil were examined at different concentrations and liquid phase temperatures by means of simple distillation and TBP distillation.The interaction mechanisms between the new activators and the crude oil or residues were analyzed,the intensifying effects by the new activators were also studied.Finally,the conceptual technique of “chemical intensification distillation”was put forward.
文摘目的探讨白细胞介素8(IL-8)对高糖诱导人脂肪间充质干细胞(h Ad MSC)损伤的保护作用。方法含250 mmol/L葡萄糖的培养基建立细胞高糖模型;P65质粒转染人IL-8基因到h Ad MSC为IL-8转染组,仅转染P65质粒者为P65对照组,在IL-8转染组中添加PD98059为Erk抑制剂组。利用MTT细胞增殖实验、ELISA或流式细胞技术检测各组h Ad MSC增殖的光密度值(OD值)、Caspase-3、Erk或VEGF等蛋白的表达。结果与P65对照组比较,IL-8转染组h Ad MSC增殖OD值明显升高,Caspase-3蛋白表达下降(P<0.01);IL-8转染组Erk蛋白活性和VEGF蛋白含量明显高于P65对照组(P<0.01);但与IL-8转染组比较,Erk抑制剂组h Ad MSC增殖的OD值降低,Caspase-3蛋白表达升高(P<0.01)。结论 IL-8在高糖环境下,通过Erk通路,发挥对h Ad MSC的保护作用。
