微自噬是真核生物体利用溶酶体直接降解蛋白质和受损细胞器的过程,在维持细胞稳态和生长发育中发挥重要作用。作为影响自噬流进程的关键复合物,内体分选转运复合体(endosomal sorting complex required for transport,ESCRT)和雷帕霉素...微自噬是真核生物体利用溶酶体直接降解蛋白质和受损细胞器的过程,在维持细胞稳态和生长发育中发挥重要作用。作为影响自噬流进程的关键复合物,内体分选转运复合体(endosomal sorting complex required for transport,ESCRT)和雷帕霉素靶蛋白复合体1(target of rapamycin complex 1,TORC1)在微自噬的调控通路中存在协同作用。本文通过系统性归纳并总结ESCRT与TORC1共同调控微自噬的分子机制,同时比较在不同类型的自噬中调控机制的差异,分析该领域仍存在的一些未解之谜,为发现调控自噬的新型分子机制提供可行性思路,为人类自噬相关疾病药物的研究提供潜在新靶点。展开更多
Two mutants of the zinc finger protein, ZNF191(243—368) I323W and R327W, are suc-cessfully obtained by site-directed mutagenesis. The fluorescence spectrum is used to study the interaction between these two mutants a...Two mutants of the zinc finger protein, ZNF191(243—368) I323W and R327W, are suc-cessfully obtained by site-directed mutagenesis. The fluorescence spectrum is used to study the interaction between these two mutants and the oligonucleotides. The influence of the mutation on the interaction has been studied using ethidium bromide (EB) as the fluorescence probe. The binding constants of the I323W-DNA and R327W-DNA have been calculated and the possible binding models have been discussed.展开更多
文摘微自噬是真核生物体利用溶酶体直接降解蛋白质和受损细胞器的过程,在维持细胞稳态和生长发育中发挥重要作用。作为影响自噬流进程的关键复合物,内体分选转运复合体(endosomal sorting complex required for transport,ESCRT)和雷帕霉素靶蛋白复合体1(target of rapamycin complex 1,TORC1)在微自噬的调控通路中存在协同作用。本文通过系统性归纳并总结ESCRT与TORC1共同调控微自噬的分子机制,同时比较在不同类型的自噬中调控机制的差异,分析该领域仍存在的一些未解之谜,为发现调控自噬的新型分子机制提供可行性思路,为人类自噬相关疾病药物的研究提供潜在新靶点。
基金This work was supported by the National Natural Science Foundation of China (Grant Nos. 30170228 and 39990600).
文摘Two mutants of the zinc finger protein, ZNF191(243—368) I323W and R327W, are suc-cessfully obtained by site-directed mutagenesis. The fluorescence spectrum is used to study the interaction between these two mutants and the oligonucleotides. The influence of the mutation on the interaction has been studied using ethidium bromide (EB) as the fluorescence probe. The binding constants of the I323W-DNA and R327W-DNA have been calculated and the possible binding models have been discussed.
