采用高效液相色谱-串联质谱法测定了猪肉中9种β-受体激动剂的残留量。样品经β-葡萄糖醛苷酶/芳基硫酸酯酶酶解后加入高氯酸沉淀蛋白并离心,取上清液过PCX阳离子固相萃取小柱净化,用氨水-甲醇(5+95)混合液洗脱,氮气吹干后用乙腈定容至1...采用高效液相色谱-串联质谱法测定了猪肉中9种β-受体激动剂的残留量。样品经β-葡萄糖醛苷酶/芳基硫酸酯酶酶解后加入高氯酸沉淀蛋白并离心,取上清液过PCX阳离子固相萃取小柱净化,用氨水-甲醇(5+95)混合液洗脱,氮气吹干后用乙腈定容至1 mL。以CAPCELL PAK CR色谱柱为分离柱,以10mmol·L-1乙酸铵溶液-乙腈(55+45)混合溶液(含体积分数为0.1%的甲酸)为流动相进行洗脱,采用电喷雾正离子源及选择反应监测模式进行测定,内标法进行定量。9种β-受体激动剂的质量浓度在4.00~100μg·L-1范围内与其峰面积呈线性关系,方法的检出限(3S/N)在0.09~0.50μg·L-1之间。对空白样品进行加标回收试验,回收率在83.2%~102%之间,测定值的相对标准偏差(n=6)在5.0%~12%之间。展开更多
[Objective] This study aimed to investigate the elimination regularity of ronidazole residues in chickens. [Method] Normal, healthy, 85-day-old Jinling broilers were selected as experimental chickens and fed with comp...[Objective] This study aimed to investigate the elimination regularity of ronidazole residues in chickens. [Method] Normal, healthy, 85-day-old Jinling broilers were selected as experimental chickens and fed with complete feed containing different concentrations of ronidazole. Ronidazole residues in chicken feather and edible tissues were detected by high performance liquid chromatography-tandem mass spectrometry （LC-MS/MS） method to compare the elimination regularity. [Result] Data analysis showed that the amount of ronidazole residues in chicken feather and edible tissues was positively correlated with the concentration of ronidazole administered. After drug withdrawal, there was a significant correlation between the elimination of ronidazole residues in chicken feather and edible tissues. Thus, the prediction model of ronidazole residues in chicken feather could be established. [Conclusion] This study could improve in vivo detection system of ronidazole residues and provide reference for monitoring and controlling drug residues in livestock and poultry oroduction.展开更多
[Objective] This study aimed to compare the residue situation of cimaterol,a kind of forbidden veterinary drug in hair, urine and flesh of pig, so as to provide theoretical basis for monitoring veterinary drug residue...[Objective] This study aimed to compare the residue situation of cimaterol,a kind of forbidden veterinary drug in hair, urine and flesh of pig, so as to provide theoretical basis for monitoring veterinary drug residue in bred animals. [Method]Total three different concentrations of cimaterol were administered to pigs, and the residue amounts of cimaterol in pig hair, urine and flesh were monitored at different raising stage. [Result] During the administration period, the residue amount of cimaterol was highest in urine, so urine is the suitable sample for rapid detection of cimaterol in manufacturing enterprises. Cimaterol could be accumulated in pig hair,where cimaterol was metabolized slowly. Thus, pig hair can be used as the test sample for tracing illegal use of veterinary drugs and for vivo detection. Flesh can be used as test sample for direct judgment whether cimaterol residue exceeds the relevant standard. [Conclusion] This study will provide certain theoretical basis for drug monitor in animal husbandry.展开更多
文摘采用高效液相色谱-串联质谱法测定了猪肉中9种β-受体激动剂的残留量。样品经β-葡萄糖醛苷酶/芳基硫酸酯酶酶解后加入高氯酸沉淀蛋白并离心,取上清液过PCX阳离子固相萃取小柱净化,用氨水-甲醇(5+95)混合液洗脱,氮气吹干后用乙腈定容至1 mL。以CAPCELL PAK CR色谱柱为分离柱,以10mmol·L-1乙酸铵溶液-乙腈(55+45)混合溶液(含体积分数为0.1%的甲酸)为流动相进行洗脱,采用电喷雾正离子源及选择反应监测模式进行测定,内标法进行定量。9种β-受体激动剂的质量浓度在4.00~100μg·L-1范围内与其峰面积呈线性关系,方法的检出限(3S/N)在0.09~0.50μg·L-1之间。对空白样品进行加标回收试验,回收率在83.2%~102%之间,测定值的相对标准偏差(n=6)在5.0%~12%之间。
文摘[Objective] This study aimed to investigate the elimination regularity of ronidazole residues in chickens. [Method] Normal, healthy, 85-day-old Jinling broilers were selected as experimental chickens and fed with complete feed containing different concentrations of ronidazole. Ronidazole residues in chicken feather and edible tissues were detected by high performance liquid chromatography-tandem mass spectrometry （LC-MS/MS） method to compare the elimination regularity. [Result] Data analysis showed that the amount of ronidazole residues in chicken feather and edible tissues was positively correlated with the concentration of ronidazole administered. After drug withdrawal, there was a significant correlation between the elimination of ronidazole residues in chicken feather and edible tissues. Thus, the prediction model of ronidazole residues in chicken feather could be established. [Conclusion] This study could improve in vivo detection system of ronidazole residues and provide reference for monitoring and controlling drug residues in livestock and poultry oroduction.
基金Supported by Key Science and Technology Program(GKZ1222003-2-2)~~
文摘[Objective] This study aimed to compare the residue situation of cimaterol,a kind of forbidden veterinary drug in hair, urine and flesh of pig, so as to provide theoretical basis for monitoring veterinary drug residue in bred animals. [Method]Total three different concentrations of cimaterol were administered to pigs, and the residue amounts of cimaterol in pig hair, urine and flesh were monitored at different raising stage. [Result] During the administration period, the residue amount of cimaterol was highest in urine, so urine is the suitable sample for rapid detection of cimaterol in manufacturing enterprises. Cimaterol could be accumulated in pig hair,where cimaterol was metabolized slowly. Thus, pig hair can be used as the test sample for tracing illegal use of veterinary drugs and for vivo detection. Flesh can be used as test sample for direct judgment whether cimaterol residue exceeds the relevant standard. [Conclusion] This study will provide certain theoretical basis for drug monitor in animal husbandry.
