目的:回顾近10年海外针灸机制研究的文献探讨该领域的研究现状并总结规律。方法:以Web of Science数据库为检索来源,采用文献计量学方法对所纳入文献的发表数量趋势、地区分布规律、文献发表刊物规律、热点领域进行分析与统计。结果:在...目的:回顾近10年海外针灸机制研究的文献探讨该领域的研究现状并总结规律。方法:以Web of Science数据库为检索来源,采用文献计量学方法对所纳入文献的发表数量趋势、地区分布规律、文献发表刊物规律、热点领域进行分析与统计。结果:在文献发表数量上,海外针灸机制研究近10年发文量相对较少,但被引用次数逐年上升;在地区分布上,韩国在文献发表总数、发表文献最多的机构和作者中都占据数量优势,美国位居其次,众多该领域高被引论文出自美国,巴西和日本紧随其后;在文献的发表刊物上以补充替代医学相关刊物为主,较少发表在以西方医学为主的综合性或专业性刊物上;疼痛、帕金森病、心脑血管疾病、抑郁、成瘾是近10年来该领域最热门的5大研究方向。结论:海外针灸机制研究的现状与规律有望为国内学者了解研究趋势和方向,把握前沿和热点提供借鉴。展开更多
Objective: Electroacupuncture (EA) in the treating principle of “soothing the liver and regulating the kidney” was applied to intervion the rats with post-traumatic stress disorder (PTSD), and its effect on anxiety-...Objective: Electroacupuncture (EA) in the treating principle of “soothing the liver and regulating the kidney” was applied to intervion the rats with post-traumatic stress disorder (PTSD), and its effect on anxiety-like behavior, spatial learning and memory ability, and expressions of synaptophysin (SYN) and postsynaptic dense 95 (PSD95) in hippocampus of rats were observed to explore the underlying mechanism.Methods: Twenty-four male SD rats were randomly divided into control group, model group, and EA group, with 8 rats in each group. There was no intervention in the control group. The rest 16 rats were prepared for modeling. The single-prolonged stress & shock (SPS&S) method was used to establish the PTSD models. There was no intervention in the model group after modeling. In the EA group, “Bǎihuì (百会GV20)”“ Shéntíng (神庭GV24)”“Gānshū (肝俞BL18)”“Shènshū (肾俞BL23)” were manipulated with EA stimulation, intensity 1 mA, frequency 2/100 Hz, disperse-dense wave, and treatment was performed once a day, 20 min each time, for a total of 21 days. Open field test, elevated plus maze and Morris water maze tests were used to observe the behavioral differences of rats in each group. Immunohistochemical method was used to observe the differences of positive expressions of proteins SYN and PSD95 in hippocampus.Results: In the open field test, compared with the control group, the total traveling distance, the percentage of the time spent in the central cell and the numbers of the central cells crossing in the model group were all decreased (all P < 0.05). Compared with the model group, these indicators in the EA group were significantly all increased (all P < 0.05). In the elevated plus maze test, compared with the control group, the percentages of open arm staying time and entering times in the model group were both decreased (both P < 0.05). Compared with the model group, these indicators in the EA group were both significantly increased (both P < 0.05). The results of Morris water maze test showed that compared with the control group, the escape latency time and travelled distance of rats in the model group were all increased from day1 to day 4 (all P < 0.05), and the percentage of staying time in the target quadrant was decreased (P < 0.05). Compared with the model group, the escape latency time and travelled distance of EA group were both significantly shortened (both P < 0.05), and the percentage of staying time in the target quadrant was significantly increased (P < 0.05). The immunohistochemical results showed that, compared with the control group, the positive expressions of SYN and PSD95 in the hippocampus of the model group were significantly down-regulated (both P < 0.05). Compared with the model group, the positive expressions of SYN and PSD95 in the hippocampus of the EA group were significantly up-regulated (both P < 0.05).Conclusions: EA in the treating principle of “soothing the liver and regulating the kidney” can effectively relieve anxiety-like behavior and improve spatial learning and memory ability of rats with PTSD, and the mechanism is related to the up-regulation of the expressions of SYN and PSD95 in the hippocampus.展开更多
文摘目的:本研究采用网状Meta分析的方法,以选择性5-羟色胺再摄取抑制剂类药物(SSRIs)作为共同参照,评价现有针灸相关疗法对比SSRIs对卒中后抑郁(PSD)的长期疗效。方法:全面检索PubMed、The Cochrane Library、EMbase、CNKI、CBM、VIP、Wan-Fang数据库中已公开发表的针灸相关疗法与SSRIs治疗PSD的临床随机对照试验,文献检索时限为各数据库建库至2020年7月31日。采用Cochrane Handbook 5.1.0对纳入研究进行偏倚风险评价。通过ADDIS、Review Manager 5.3、STATA 13.1软件进行数据分析。结果:纳入30个RCT,共计3115例PSD患者,涉及10种治疗方式。网状Meta分析结果显示:在总有效率方面,体针+SSRIs优于体针[OR=2.85,95%CI(1.51,5.90)]、SSRIs[OR=5.37,95%CI(3.03,10.33)];在汉密顿抑郁量表(Hamilton Depression Scale,HAMD)评分方面,体针+SSRIs优于体针[MD=1.69,95%CI(0.33,3.06)]、SSRIs[MD=3.87,95%CI(2.68,5.08)],以上排序预测以艾灸+SSRIs为最优;在美国国立卫生研究院卒中量表(National Institute of Health stroke scale,NIHSS)评分方面,体针[MD=2.15,95%CI(1.10,3.26)]、体针+SSRIs[MD=1.77,95%CI(0.19,3.37)]均优于SSRIs。结论:针灸相关疗法对PSD的长期疗效,以艾灸联合SSRIs最优,体针及与SSRIs联合运用优于SSRIs单独运用,其余疗法之间各有优劣。基于现有研究的缺陷,本结论尚需更多高质量随机对照试验(RCTs)予以验证。
文摘目的:回顾近10年海外针灸机制研究的文献探讨该领域的研究现状并总结规律。方法:以Web of Science数据库为检索来源,采用文献计量学方法对所纳入文献的发表数量趋势、地区分布规律、文献发表刊物规律、热点领域进行分析与统计。结果:在文献发表数量上,海外针灸机制研究近10年发文量相对较少,但被引用次数逐年上升;在地区分布上,韩国在文献发表总数、发表文献最多的机构和作者中都占据数量优势,美国位居其次,众多该领域高被引论文出自美国,巴西和日本紧随其后;在文献的发表刊物上以补充替代医学相关刊物为主,较少发表在以西方医学为主的综合性或专业性刊物上;疼痛、帕金森病、心脑血管疾病、抑郁、成瘾是近10年来该领域最热门的5大研究方向。结论:海外针灸机制研究的现状与规律有望为国内学者了解研究趋势和方向,把握前沿和热点提供借鉴。
基金Supported by National Natural Science Foundation of China:81873384。
文摘Objective: Electroacupuncture (EA) in the treating principle of “soothing the liver and regulating the kidney” was applied to intervion the rats with post-traumatic stress disorder (PTSD), and its effect on anxiety-like behavior, spatial learning and memory ability, and expressions of synaptophysin (SYN) and postsynaptic dense 95 (PSD95) in hippocampus of rats were observed to explore the underlying mechanism.Methods: Twenty-four male SD rats were randomly divided into control group, model group, and EA group, with 8 rats in each group. There was no intervention in the control group. The rest 16 rats were prepared for modeling. The single-prolonged stress & shock (SPS&S) method was used to establish the PTSD models. There was no intervention in the model group after modeling. In the EA group, “Bǎihuì (百会GV20)”“ Shéntíng (神庭GV24)”“Gānshū (肝俞BL18)”“Shènshū (肾俞BL23)” were manipulated with EA stimulation, intensity 1 mA, frequency 2/100 Hz, disperse-dense wave, and treatment was performed once a day, 20 min each time, for a total of 21 days. Open field test, elevated plus maze and Morris water maze tests were used to observe the behavioral differences of rats in each group. Immunohistochemical method was used to observe the differences of positive expressions of proteins SYN and PSD95 in hippocampus.Results: In the open field test, compared with the control group, the total traveling distance, the percentage of the time spent in the central cell and the numbers of the central cells crossing in the model group were all decreased (all P < 0.05). Compared with the model group, these indicators in the EA group were significantly all increased (all P < 0.05). In the elevated plus maze test, compared with the control group, the percentages of open arm staying time and entering times in the model group were both decreased (both P < 0.05). Compared with the model group, these indicators in the EA group were both significantly increased (both P < 0.05). The results of Morris water maze test showed that compared with the control group, the escape latency time and travelled distance of rats in the model group were all increased from day1 to day 4 (all P < 0.05), and the percentage of staying time in the target quadrant was decreased (P < 0.05). Compared with the model group, the escape latency time and travelled distance of EA group were both significantly shortened (both P < 0.05), and the percentage of staying time in the target quadrant was significantly increased (P < 0.05). The immunohistochemical results showed that, compared with the control group, the positive expressions of SYN and PSD95 in the hippocampus of the model group were significantly down-regulated (both P < 0.05). Compared with the model group, the positive expressions of SYN and PSD95 in the hippocampus of the EA group were significantly up-regulated (both P < 0.05).Conclusions: EA in the treating principle of “soothing the liver and regulating the kidney” can effectively relieve anxiety-like behavior and improve spatial learning and memory ability of rats with PTSD, and the mechanism is related to the up-regulation of the expressions of SYN and PSD95 in the hippocampus.
