RNA polymerase Ⅱ from yeast cannot transcribe cDNA of potato spindle tuber viroid (PSTV), in spite of its ability to transcribe in vitro PSTV faithfully. RNA polymerase from E. coli is capable of transcribing both PS...RNA polymerase Ⅱ from yeast cannot transcribe cDNA of potato spindle tuber viroid (PSTV), in spite of its ability to transcribe in vitro PSTV faithfully. RNA polymerase from E. coli is capable of transcribing both PSTV and its cDNA. PAGE analysis of transcripts, Southern molecular hybridization and experiments to exclude the possibility of terminal labeling of template indicate that the transcription of PSTV cDNA by E. coli RNA polymerase is a faithful one. This paper first presents the idea that PSTV cDNA constitutes a transcriptional unit containing a promoter-like region capable of directing specific initiation of transcription. It is proved that the promoter is located on the 132 bp small fragment obtained after cleavage of PSTV cDNAwith AvaII, probably lying around the BamHl site in the 5’terminus.展开更多
基金Project supported by the National Natural Science Foundation of China
文摘RNA polymerase Ⅱ from yeast cannot transcribe cDNA of potato spindle tuber viroid (PSTV), in spite of its ability to transcribe in vitro PSTV faithfully. RNA polymerase from E. coli is capable of transcribing both PSTV and its cDNA. PAGE analysis of transcripts, Southern molecular hybridization and experiments to exclude the possibility of terminal labeling of template indicate that the transcription of PSTV cDNA by E. coli RNA polymerase is a faithful one. This paper first presents the idea that PSTV cDNA constitutes a transcriptional unit containing a promoter-like region capable of directing specific initiation of transcription. It is proved that the promoter is located on the 132 bp small fragment obtained after cleavage of PSTV cDNAwith AvaII, probably lying around the BamHl site in the 5’terminus.
