为高效地提取桑叶黄酮,采用正交试验法和响应面法就提取时间、提取温度、乙醇浓度和液料比4个因素进行桑叶黄酮提取工艺的优化.正交优化法所得最佳提取工艺为:提取温度60℃、提取时间60 min、乙醇浓度50%和料液比100 m L/g,此条件下桑...为高效地提取桑叶黄酮,采用正交试验法和响应面法就提取时间、提取温度、乙醇浓度和液料比4个因素进行桑叶黄酮提取工艺的优化.正交优化法所得最佳提取工艺为:提取温度60℃、提取时间60 min、乙醇浓度50%和料液比100 m L/g,此条件下桑叶黄酮的得率为4.53%.响应面优化法所得最佳提取工艺为:提取温度68℃、提取时间40 min、乙醇浓度52%和液料比86 m L/g,此条件下桑叶黄酮得率为4.71%,而理论预测值为4.64%.结果表明:响应面法优化的工艺条件下得到的桑叶黄酮量高于正交试验法,生产能耗也低于后者;并且响应面法建立的优化模型也能较好地预测桑叶黄酮的提取率.展开更多
[Objective] The aim was to study on the effects of long-term low radiation on canopy apparent photosynthetic rate （CAP） and photosynthetic properties of top three leaves of winter wheat, especially the flag leaf. [M...[Objective] The aim was to study on the effects of long-term low radiation on canopy apparent photosynthetic rate （CAP） and photosynthetic properties of top three leaves of winter wheat, especially the flag leaf. [Method] Two winter wheat （Triticum aestivum L） cultivars, Yangmai 158 and Yangmai 11, which differed in low radiation resistance, were used to measure the CAP, photosynthetic rate （Pn） of the top three leaves, chlorophyll content of flag leaf and grain yield. In the test, three treatments were designed from jointing to maturity, as follows： control without shad- ing （So）, S1 and S2 treatments with 22% and 33% sunshine shaded. [Result] CAP of Yangmai 158 and Yangmai 11 declined with shading. Under low radiation, Pn of flag leaf declined significantly, while no obvious effects were found on Pn of the 2nd leaf, and Pn of the 3rn leaf increased significantly, which partially compensated the decrease of Pn of the flag leaf. In addition, the compensation effect differed in shading and cultivars： compensation effect in S1 group was higher than that of S2 and effect of Yangmai 158 was higher than that of Yangmai 11. During the period of high Pn. shading decreased the content of total chlorophyll, chlorophyll a, b, and a/b. [Conclusion] Under low radiation, the decrease of chlorophyll a and a/b led to substantial declining of Pn. and CAP declined accordingly, finally resulting in drop of grain yield. The research provides important theoretical basis for wheat production in the middle and lower reaches of Yangtze River.展开更多
文摘为高效地提取桑叶黄酮,采用正交试验法和响应面法就提取时间、提取温度、乙醇浓度和液料比4个因素进行桑叶黄酮提取工艺的优化.正交优化法所得最佳提取工艺为:提取温度60℃、提取时间60 min、乙醇浓度50%和料液比100 m L/g,此条件下桑叶黄酮的得率为4.53%.响应面优化法所得最佳提取工艺为:提取温度68℃、提取时间40 min、乙醇浓度52%和液料比86 m L/g,此条件下桑叶黄酮得率为4.71%,而理论预测值为4.64%.结果表明:响应面法优化的工艺条件下得到的桑叶黄酮量高于正交试验法,生产能耗也低于后者;并且响应面法建立的优化模型也能较好地预测桑叶黄酮的提取率.
基金Supported by Natural Science Foundation of the Higher Education Institutions of Jiangsu Province (10KJB210002)China Postdoctoral Science Foundation (20110491442)Jiangsu Planned Projects for Postdoctoral Research Funds (1101059C)~~
文摘[Objective] The aim was to study on the effects of long-term low radiation on canopy apparent photosynthetic rate （CAP） and photosynthetic properties of top three leaves of winter wheat, especially the flag leaf. [Method] Two winter wheat （Triticum aestivum L） cultivars, Yangmai 158 and Yangmai 11, which differed in low radiation resistance, were used to measure the CAP, photosynthetic rate （Pn） of the top three leaves, chlorophyll content of flag leaf and grain yield. In the test, three treatments were designed from jointing to maturity, as follows： control without shad- ing （So）, S1 and S2 treatments with 22% and 33% sunshine shaded. [Result] CAP of Yangmai 158 and Yangmai 11 declined with shading. Under low radiation, Pn of flag leaf declined significantly, while no obvious effects were found on Pn of the 2nd leaf, and Pn of the 3rn leaf increased significantly, which partially compensated the decrease of Pn of the flag leaf. In addition, the compensation effect differed in shading and cultivars： compensation effect in S1 group was higher than that of S2 and effect of Yangmai 158 was higher than that of Yangmai 11. During the period of high Pn. shading decreased the content of total chlorophyll, chlorophyll a, b, and a/b. [Conclusion] Under low radiation, the decrease of chlorophyll a and a/b led to substantial declining of Pn. and CAP declined accordingly, finally resulting in drop of grain yield. The research provides important theoretical basis for wheat production in the middle and lower reaches of Yangtze River.
