To investigate the radioimmunotherapeutic efficacy, radio-immunoconjugate 131-I-3G9, 811-I-3H11 and 131-I-NMIgG (irre levant antibody) were i.p. injected into nude mice bearing human gastric cancer xenograftes. Each a...To investigate the radioimmunotherapeutic efficacy, radio-immunoconjugate 131-I-3G9, 811-I-3H11 and 131-I-NMIgG (irre levant antibody) were i.p. injected into nude mice bearing human gastric cancer xenograftes. Each animal received a single doses of 555MBq. Over 14 days the accumulative absorbed doses in tumors were 13.7 Gy for 131-I-3H11 and 12.17 Gy for 131-I-3G9. Both were significantly higher than that for 131-I-NMIgG (3.23 Gy). Thera peutic efficacy appeared most sharply from 2 to 3 weeks after injection. The inhibition ratio of tumor were 86% and 70% for 131-I-3H11 and 131-I-3G9 respectively. Histopathological evidance indicated that in tumor tissues radioactive damage was showed as karyopyknosis, karyorrhexis and necrosis or partial disappearance of tumor cells, while in the other tissues no radioactive damage was observed. WBC counts of all animals did not show significant difference before and after treatment, which indicated that the haemopoietic function of bone marrow was not affected.展开更多
The Schwartz technique is a simple method for labeling McAb directly with ￣(99m)Tc and can he utilized to design a one-step radiopharmacelltical kit.In this study,it was applied to label anti-human gastric cancer McA...The Schwartz technique is a simple method for labeling McAb directly with ￣(99m)Tc and can he utilized to design a one-step radiopharmacelltical kit.In this study,it was applied to label anti-human gastric cancer McAb 3H11. The antibody was reduced at room temperature for 30 min with a 1000-fold molar ratio excess of 2mercaptoethanol. The mean labeling efficiency of ￣(99m)Tc3H11 was more than 95%. The immunoreactivity of ￣(99m)Tc-3H11 was more than 80% by ELISA's method. Competition results in vitro showed that ￣(99m)Tc was combined with the high affinity sites of antibody by HPLC. The biodistribution in nude mice bearing 823 gastric cancer xenographts showed that the radioactivity of tumor uptake at 24 h post-injection was the highest except for kidney.The tumor uptake was 8.98±2.42% i.d/g.The count ratio of tumor to blood was over 1.5 and that tumor to liver was more than 2.5 at 24 h post-injection.And the tumor was clearly imaged at 22 h post-injection. The initial clinical results showed that￣(99m)Tc-3H11 was stable in vivo and well good located at the lesion sites.展开更多
In this article, the significance of radioimmunoimaging(RII)as a mornitoring means for gastric colorectal and lung cancer patients was reported.By using I-131 or Tc-99m labeled C50(antiCEA)and 3H11(anti human gastric ...In this article, the significance of radioimmunoimaging(RII)as a mornitoring means for gastric colorectal and lung cancer patients was reported.By using I-131 or Tc-99m labeled C50(antiCEA)and 3H11(anti human gastric cancer), 86 positive lesions were identified in 50 cancer patiens.77 out of the 86 lesions (89.5%)were true positive, 4 false positive by post-surgecal pathology,biopsy or CT scan.The other 5 remained to be unexplained and needed further follow-up.In our clinical practice,RII with antitumor McAbs suggested its unique value in the management of cancer petients.Totally, 14 occult lesions in 9 cancer petients missed by to other examination were identified by this technique.And owing to the RII findings,all of these patients got proper treatment in time.展开更多
文摘对接受放射性核素标记鼠源性单克隆抗体(简称单抗)C50作放射免疫显像(RII)或放射免疫治疗(RIT)的患者,用间接酶联免疫吸附法(EIASA)检测其外周血中人抗鼠抗体(HAMA),结合单抗应用剂量,分析 HAMA 产生的规律及相应的对策。资料与方法1.抗体及其核素标记。抗 CEA 单抗 C50由卫生部北京生物制品研究所提供。C50是鼠源性。对 CEA
文摘To investigate the radioimmunotherapeutic efficacy, radio-immunoconjugate 131-I-3G9, 811-I-3H11 and 131-I-NMIgG (irre levant antibody) were i.p. injected into nude mice bearing human gastric cancer xenograftes. Each animal received a single doses of 555MBq. Over 14 days the accumulative absorbed doses in tumors were 13.7 Gy for 131-I-3H11 and 12.17 Gy for 131-I-3G9. Both were significantly higher than that for 131-I-NMIgG (3.23 Gy). Thera peutic efficacy appeared most sharply from 2 to 3 weeks after injection. The inhibition ratio of tumor were 86% and 70% for 131-I-3H11 and 131-I-3G9 respectively. Histopathological evidance indicated that in tumor tissues radioactive damage was showed as karyopyknosis, karyorrhexis and necrosis or partial disappearance of tumor cells, while in the other tissues no radioactive damage was observed. WBC counts of all animals did not show significant difference before and after treatment, which indicated that the haemopoietic function of bone marrow was not affected.
文摘The Schwartz technique is a simple method for labeling McAb directly with ￣(99m)Tc and can he utilized to design a one-step radiopharmacelltical kit.In this study,it was applied to label anti-human gastric cancer McAb 3H11. The antibody was reduced at room temperature for 30 min with a 1000-fold molar ratio excess of 2mercaptoethanol. The mean labeling efficiency of ￣(99m)Tc3H11 was more than 95%. The immunoreactivity of ￣(99m)Tc-3H11 was more than 80% by ELISA's method. Competition results in vitro showed that ￣(99m)Tc was combined with the high affinity sites of antibody by HPLC. The biodistribution in nude mice bearing 823 gastric cancer xenographts showed that the radioactivity of tumor uptake at 24 h post-injection was the highest except for kidney.The tumor uptake was 8.98±2.42% i.d/g.The count ratio of tumor to blood was over 1.5 and that tumor to liver was more than 2.5 at 24 h post-injection.And the tumor was clearly imaged at 22 h post-injection. The initial clinical results showed that￣(99m)Tc-3H11 was stable in vivo and well good located at the lesion sites.
文摘In this article, the significance of radioimmunoimaging(RII)as a mornitoring means for gastric colorectal and lung cancer patients was reported.By using I-131 or Tc-99m labeled C50(antiCEA)and 3H11(anti human gastric cancer), 86 positive lesions were identified in 50 cancer patiens.77 out of the 86 lesions (89.5%)were true positive, 4 false positive by post-surgecal pathology,biopsy or CT scan.The other 5 remained to be unexplained and needed further follow-up.In our clinical practice,RII with antitumor McAbs suggested its unique value in the management of cancer petients.Totally, 14 occult lesions in 9 cancer petients missed by to other examination were identified by this technique.And owing to the RII findings,all of these patients got proper treatment in time.
