[Objective] The experiment explored of resistance of different Medicago sativa through synthetic variations of all physiological indexes and the correlation analysis between physiological activity and damage degree af...[Objective] The experiment explored of resistance of different Medicago sativa through synthetic variations of all physiological indexes and the correlation analysis between physiological activity and damage degree after thrips damage. [Method] According to studying physiological activity (POD activity, SOD activity, PPO activity, PAL activity, MDA content and free proline content) of alfalfa in alfalfa resistance to different number of thrips, the correlation between physiological activity and resistance was investigated. [Result] It was shown that there were negative correlations among POD, SOD, PPO, PAL, MDA, free praline and the number of thrips, and there were negative correlations among POD, SOD, PPO, PAL, MDA, free praline and CAT. The content changes of POD,SOD,PPO,CAT,MDA and free proline in high resistant alfalfa were slow and PAL was quick and the activities of PPO,PAL,CAT were high, and the activities of POD、SOD were low. [Conclusion] The research provided theoretical basis and materials for identifying resistance varieties of alfalfa to thrip.展开更多
蓟马是紫花苜蓿的主要害虫之一,严重影响苜蓿的产量和品质,进而影响苜蓿产业的健康发展。以草原2号(Medicago varia Martin.Caoyuan No.2)感蓟马品种、草原4号(Medicago sativa L.Caoyuan No.4)抗蓟马品种为材料,分别进行牛角花齿蓟马(O...蓟马是紫花苜蓿的主要害虫之一,严重影响苜蓿的产量和品质,进而影响苜蓿产业的健康发展。以草原2号(Medicago varia Martin.Caoyuan No.2)感蓟马品种、草原4号(Medicago sativa L.Caoyuan No.4)抗蓟马品种为材料,分别进行牛角花齿蓟马(Odontothrips loti)接虫和不接虫处理,利用广泛靶向代谢组学技术,分析蓟马取食诱导的苜蓿代谢物变化,从代谢水平探究蓟马取食引起的苜蓿诱导性防御机制。结果表明,蓟马取食会诱导苜蓿初生代谢物和次生代谢物发生变化。感蓟马品种草原2号苜蓿在蓟马取食前后的差异代谢物为178个(S-CK vs S-T),而抗蓟马品种草原4号苜蓿在蓟马取食前后的差异代谢物为116个(R-CK vs R-T),有60个差异代谢物在2个品种中都受到蓟马取食影响。在共同诱导的代谢物中,主要包括31个黄酮类化合物、7个有机酸及衍生物、7苯丙素和5个氨基酸及衍生物等12大类。蓟马取食诱导的代谢物变化主要集中在黄酮类化合物,包括麦黄酮、木樨草素、白杨素、芹菜苷等,说明黄酮类化合物在苜蓿诱导性防御中可能具有重要的作用,可为进一步开展苜蓿抗蓟马诱导性防御提供参考。展开更多
基金Supported by the Ministry of Science and Technology of the People’s Republic of China(2006BAD10A19-8)~~
文摘[Objective] The experiment explored of resistance of different Medicago sativa through synthetic variations of all physiological indexes and the correlation analysis between physiological activity and damage degree after thrips damage. [Method] According to studying physiological activity (POD activity, SOD activity, PPO activity, PAL activity, MDA content and free proline content) of alfalfa in alfalfa resistance to different number of thrips, the correlation between physiological activity and resistance was investigated. [Result] It was shown that there were negative correlations among POD, SOD, PPO, PAL, MDA, free praline and the number of thrips, and there were negative correlations among POD, SOD, PPO, PAL, MDA, free praline and CAT. The content changes of POD,SOD,PPO,CAT,MDA and free proline in high resistant alfalfa were slow and PAL was quick and the activities of PPO,PAL,CAT were high, and the activities of POD、SOD were low. [Conclusion] The research provided theoretical basis and materials for identifying resistance varieties of alfalfa to thrip.
文摘蓟马是紫花苜蓿的主要害虫之一,严重影响苜蓿的产量和品质,进而影响苜蓿产业的健康发展。以草原2号(Medicago varia Martin.Caoyuan No.2)感蓟马品种、草原4号(Medicago sativa L.Caoyuan No.4)抗蓟马品种为材料,分别进行牛角花齿蓟马(Odontothrips loti)接虫和不接虫处理,利用广泛靶向代谢组学技术,分析蓟马取食诱导的苜蓿代谢物变化,从代谢水平探究蓟马取食引起的苜蓿诱导性防御机制。结果表明,蓟马取食会诱导苜蓿初生代谢物和次生代谢物发生变化。感蓟马品种草原2号苜蓿在蓟马取食前后的差异代谢物为178个(S-CK vs S-T),而抗蓟马品种草原4号苜蓿在蓟马取食前后的差异代谢物为116个(R-CK vs R-T),有60个差异代谢物在2个品种中都受到蓟马取食影响。在共同诱导的代谢物中,主要包括31个黄酮类化合物、7个有机酸及衍生物、7苯丙素和5个氨基酸及衍生物等12大类。蓟马取食诱导的代谢物变化主要集中在黄酮类化合物,包括麦黄酮、木樨草素、白杨素、芹菜苷等,说明黄酮类化合物在苜蓿诱导性防御中可能具有重要的作用,可为进一步开展苜蓿抗蓟马诱导性防御提供参考。
