目的:通过体外实验评价植酸(IP6)作为根管螯合剂去除次氯酸钠溶液处理的牙本质表面玷污层的效力,探究其对次氯酸钠溶液处理的牙本质化学组成的影响。方法:24个完整无龋正畸牙取得牙本质样本随机分成4组。A组:2mL去离子水(pH7.4)处理5mi...目的:通过体外实验评价植酸(IP6)作为根管螯合剂去除次氯酸钠溶液处理的牙本质表面玷污层的效力,探究其对次氯酸钠溶液处理的牙本质化学组成的影响。方法:24个完整无龋正畸牙取得牙本质样本随机分成4组。A组:2mL去离子水(pH7.4)处理5min;B组:2 mL 5%NaClO溶液(pH12)处理5 min;C组:2 mL 5%NaClO溶液处理5min后,2mL 17%EDTA溶液(pH7.5)处理1 min;D组:2 mL 5%NaClO溶液处理5 min后,2mL 1%IP6溶液(pH1.3)处理1min。SEM观察样本表面玷污层去除情况,衰减全反射红外光谱仪(ATR-IR)测量样本红外光谱,得到处理前后的M:M(matrix:mineral)和C:M(carbonate:mineral)。以双因素重复测量方差分析比较差异。结果:SEM显示处理后A组仍有大量玷污层剩余,B组玷污层去除效果不明显,C组玷污层基本完全去除,D组玷污去除效果更加明显。ATR-IR结果经统计分析后发现,A组M:M处理前后无显著差异(P=0.744),B、C、D组均显著下降(P<0.01);处理后B、C、D组明显低于A组(P<0.01),而该3组间无显著差异(P>0.05);四组C:M处理后均略低于处理前(P<0.01)。结论:植酸能够有效地去除经次氯酸钠溶液处理后的牙本质表面的玷污层,并且其与EDTA对牙本质本身化学组成的影响无显著差别。展开更多
This study evaluated the effects of sodium hypochlorite（NaOCl） with different concentrations and exposure time on the structural, compositional and mechanical properties of human dentin in vitro. Sixty dentin slabs ...This study evaluated the effects of sodium hypochlorite（NaOCl） with different concentrations and exposure time on the structural, compositional and mechanical properties of human dentin in vitro. Sixty dentin slabs were obtained from freshly extracted premolars, randomly distributed into four groups（n=15）, and treated with 1%, 5%, 10% NaOCl and distilled water（control group）, respectively, for a total of 60 min. Attenuated total reflection infrared（ATR-IR） spectroscopy, Raman spectroscopy and X-ray diffraction（XRD） were carried out before, 10 min and 60 min after the treatment. Scanning electron microscopy（SEM） and flexural strength test were conducted as well. The results showed that dentins experienced morphological alterations in the NaOCl groups, but not in the control group. Two-way repeated-measures analysis of variance revealed that the carbonate：mineral ratio（C：M）, Raman relative intensity（RRI）, a-axis, c-axis length and full width at half maximum（FWHM） with the increase of time and concentration in the NaOCl groups were not significantly different from those in the control group（P〉0.05）. Nevertheless, the mineral：matrix ratio（M：M） increased and the flexural strength declined with the increase of concentration and the extension of time in the NaOCl groups（P〈0.05）. Additionally, it was found that the M：M and the flexural strength remained unchanged after 1% NaOCl treatment（P〉0.05）, and the morphology changes were unnoticeable within 10 min in 1% NaOCl group. These results indicated that NaOCl has no significant effects on the inorganic mineral of human dentin; but it undermines and eliminates the organic content concentration-and time-dependently, which in turn influences the flexural strength and toughness of dentins. In addition, an irrigation of 1% NaOCl within 10 min can minimize the effects of NaOCl on the structural and mechanical properties of dentin during root canal treatment.展开更多
文摘目的:通过体外实验评价植酸(IP6)作为根管螯合剂去除次氯酸钠溶液处理的牙本质表面玷污层的效力,探究其对次氯酸钠溶液处理的牙本质化学组成的影响。方法:24个完整无龋正畸牙取得牙本质样本随机分成4组。A组:2mL去离子水(pH7.4)处理5min;B组:2 mL 5%NaClO溶液(pH12)处理5 min;C组:2 mL 5%NaClO溶液处理5min后,2mL 17%EDTA溶液(pH7.5)处理1 min;D组:2 mL 5%NaClO溶液处理5 min后,2mL 1%IP6溶液(pH1.3)处理1min。SEM观察样本表面玷污层去除情况,衰减全反射红外光谱仪(ATR-IR)测量样本红外光谱,得到处理前后的M:M(matrix:mineral)和C:M(carbonate:mineral)。以双因素重复测量方差分析比较差异。结果:SEM显示处理后A组仍有大量玷污层剩余,B组玷污层去除效果不明显,C组玷污层基本完全去除,D组玷污去除效果更加明显。ATR-IR结果经统计分析后发现,A组M:M处理前后无显著差异(P=0.744),B、C、D组均显著下降(P<0.01);处理后B、C、D组明显低于A组(P<0.01),而该3组间无显著差异(P>0.05);四组C:M处理后均略低于处理前(P<0.01)。结论:植酸能够有效地去除经次氯酸钠溶液处理后的牙本质表面的玷污层,并且其与EDTA对牙本质本身化学组成的影响无显著差别。
基金supported by the National Natural Science Foundation of China(No.81470771,No.81500887)the Natural Science Foundation of Hubei Province(No.2013CFA068)
文摘This study evaluated the effects of sodium hypochlorite（NaOCl） with different concentrations and exposure time on the structural, compositional and mechanical properties of human dentin in vitro. Sixty dentin slabs were obtained from freshly extracted premolars, randomly distributed into four groups（n=15）, and treated with 1%, 5%, 10% NaOCl and distilled water（control group）, respectively, for a total of 60 min. Attenuated total reflection infrared（ATR-IR） spectroscopy, Raman spectroscopy and X-ray diffraction（XRD） were carried out before, 10 min and 60 min after the treatment. Scanning electron microscopy（SEM） and flexural strength test were conducted as well. The results showed that dentins experienced morphological alterations in the NaOCl groups, but not in the control group. Two-way repeated-measures analysis of variance revealed that the carbonate：mineral ratio（C：M）, Raman relative intensity（RRI）, a-axis, c-axis length and full width at half maximum（FWHM） with the increase of time and concentration in the NaOCl groups were not significantly different from those in the control group（P〉0.05）. Nevertheless, the mineral：matrix ratio（M：M） increased and the flexural strength declined with the increase of concentration and the extension of time in the NaOCl groups（P〈0.05）. Additionally, it was found that the M：M and the flexural strength remained unchanged after 1% NaOCl treatment（P〉0.05）, and the morphology changes were unnoticeable within 10 min in 1% NaOCl group. These results indicated that NaOCl has no significant effects on the inorganic mineral of human dentin; but it undermines and eliminates the organic content concentration-and time-dependently, which in turn influences the flexural strength and toughness of dentins. In addition, an irrigation of 1% NaOCl within 10 min can minimize the effects of NaOCl on the structural and mechanical properties of dentin during root canal treatment.
基金supported by the Natural Science Foundation of Shanghai Science and Technology CommitteeChina(No.10ZR1406000)+1 种基金the Open Research Fund Program of the Institutes of Brain ScienceFudan University(2008-2010)
