This study was conducted to optimize the hydrolysis of porcine serum protein by response surface methodology (RSM). Based on single-factor tests, 3- factor 3-level response surface tests were designed by Box-Behnken...This study was conducted to optimize the hydrolysis of porcine serum protein by response surface methodology (RSM). Based on single-factor tests, 3- factor 3-level response surface tests were designed by Box-Behnken design method, and the effects of pH, temperature and hydrolysis duration on the response values were analyzed by RSM method. The optimal enzymolysis conditions were obtained as follows: pH value of 7.7, temperature at 46.3 ℃ and time of 7.4 h, under which the predicted degree of hydrolysis was 35.52%, and the chelating rate was 79.41%. This process could provide new ideas for the development of porcine serum protein.展开更多
文摘This study was conducted to optimize the hydrolysis of porcine serum protein by response surface methodology (RSM). Based on single-factor tests, 3- factor 3-level response surface tests were designed by Box-Behnken design method, and the effects of pH, temperature and hydrolysis duration on the response values were analyzed by RSM method. The optimal enzymolysis conditions were obtained as follows: pH value of 7.7, temperature at 46.3 ℃ and time of 7.4 h, under which the predicted degree of hydrolysis was 35.52%, and the chelating rate was 79.41%. This process could provide new ideas for the development of porcine serum protein.