To investigate the role of IL-18 and IL-18 binding protein (IL-18BP) in the pathogenesis of psoriasis, PT-PCR was used to semi-quantitatively analyze the mRNA expression of IL-18, AcPL (accessory protein-like) and I...To investigate the role of IL-18 and IL-18 binding protein (IL-18BP) in the pathogenesis of psoriasis, PT-PCR was used to semi-quantitatively analyze the mRNA expression of IL-18, AcPL (accessory protein-like) and IL-18BP in 12 psoriatic lesions and in 6 normal skin tissues. The results showed that IL-18 and AcPL mRNA were present in all specimens from 6 normal skin and 12 pieces of psoriatic skin, and IL-18 and AcPL showed relatively stronger intensity in the psoriatic skin compared with those seen in normal skin (P<0.01). Amplified bands indicating the expression of IL-18BP mRNA were weakly positive in all samples of normal skin. In contrast, the PCR products for IL-18BP were distinctly visible in all specimens of psoriatic lesions (P<0.01). These findings demonstrate that IL-18 is constitutively synthesized by human keratinocytes and involved in the development of the Th1 response in psoriatic lesions, and its bioactivity appears to be closely regulated by cutaneous inflammation.展开更多
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患者女，56岁。自幼发现右侧腰部褐色斑块，无自觉症状，随年龄的增长缓慢增大，逐渐隆起，触之柔软，从未诊治。近10天来，皮损中央出现红肿疼痛，无溃烂，未诊治，因红肿加重，到我科就诊。病程中无特殊病史，精神食欲，睡眠尚可，大小便正常。既往体健，无特殊。家族中无类似病史，无遗传病史。查体：一般情况尚可，全身浅表淋巴结未触及肿大，系统检查无异常。皮肤专科情况：右侧腰部见一6.0 cm ×5.0；cm ×2.0cm大小，深浅不同的褐色桑椹样肿块，中央红肿，表面无破溃，肿块触之较柔软（图1）。实验室检查：血常规正常。皮损边缘取材做普通病理显示：表皮基底层色素增加，真皮浅层结缔组织疏松，真皮中下层大量的痣细胞巢，有成熟现象，无细胞异型性（图2）。皮损中央红肿处，给莫匹罗星软膏外用，一周后，红肿疼痛完全消退（图3）。诊断：皮内痣并感染。治疗：建议患者手术切除。
文摘To investigate the role of IL-18 and IL-18 binding protein (IL-18BP) in the pathogenesis of psoriasis, PT-PCR was used to semi-quantitatively analyze the mRNA expression of IL-18, AcPL (accessory protein-like) and IL-18BP in 12 psoriatic lesions and in 6 normal skin tissues. The results showed that IL-18 and AcPL mRNA were present in all specimens from 6 normal skin and 12 pieces of psoriatic skin, and IL-18 and AcPL showed relatively stronger intensity in the psoriatic skin compared with those seen in normal skin (P<0.01). Amplified bands indicating the expression of IL-18BP mRNA were weakly positive in all samples of normal skin. In contrast, the PCR products for IL-18BP were distinctly visible in all specimens of psoriatic lesions (P<0.01). These findings demonstrate that IL-18 is constitutively synthesized by human keratinocytes and involved in the development of the Th1 response in psoriatic lesions, and its bioactivity appears to be closely regulated by cutaneous inflammation.
