In order to investigate the mechanism of ROS signaling transduction in after-germination process, we screened a hydrogen peroxide sensitive mutant from EMS mutant library of Arabidopsis thaliana. Hydrogen peroxide sen...In order to investigate the mechanism of ROS signaling transduction in after-germination process, we screened a hydrogen peroxide sensitive mutant from EMS mutant library of Arabidopsis thaliana. Hydrogen peroxide sensitive 12（hps12）was screened from this library. Phenotype analysis indicated that hps12 had dwarf plant and shorter pod than wild type（WT）. The cotyledon greening rate of hps12 was decreased after being treated with 4 mmol/L H_2O_2. Furthermore, the isolated leaves of hps12 mutants showed more obvious symptoms of senescence than WT under the treatment of 10 mmol/L H_2O_2. Genetic analysis suggested that hps12 was a monogenic recessive mutant.展开更多
已知许多蛋白激酶及蛋白磷酸酶参与了ABA诱导的气孔关闭信号转导过程,而H_2O_2是ABA信号转导链的下游信号成分。运用表皮条生物学分析和激光共聚焦扫描技术,研究促细胞分裂原活化蛋白激酶(mitogen-activated protein kinase,MAPK)对蚕...已知许多蛋白激酶及蛋白磷酸酶参与了ABA诱导的气孔关闭信号转导过程,而H_2O_2是ABA信号转导链的下游信号成分。运用表皮条生物学分析和激光共聚焦扫描技术,研究促细胞分裂原活化蛋白激酶(mitogen-activated protein kinase,MAPK)对蚕豆保卫细胞中ABA诱导H_2O_2产生的调节作用。用MEK1/2专一抑制剂(PD98059)处理蚕豆叶片的下表皮,抑制了ABA诱导保卫细胞内H_2O_2的产生和气孔关闭。ABA和H_2O_2诱导气孔关闭后,再用PD98059处理,可以使关闭的气孔重新开放,与之相对应的是PD98059使ABA诱导的、已增强了的H_2O_2探针荧光强度降低。而且PD98059不能阻断水杨酸诱导的H_2O_2的产生及其气孔的关闭,因此,MEH1/2调节ABA诱导保卫细胞中H_2O_2产生和积累具有专一性。总之,PD98059通过抑制ABA诱导的H_2O_2的产生并清除其积累而阻断了ABA诱导的气孔关闭。因此,MAPK的激活在保卫细胞H_2O_2信号的产生、放大及其专一性应答信号刺激的反应中有着重要的调节作用。展开更多
基金Supported by National Natural Science Foundation of China(31170253)Key Scientific Research Foundation of the Higher Education Institutions of Henan Province,China(15A180001)~~
文摘In order to investigate the mechanism of ROS signaling transduction in after-germination process, we screened a hydrogen peroxide sensitive mutant from EMS mutant library of Arabidopsis thaliana. Hydrogen peroxide sensitive 12（hps12）was screened from this library. Phenotype analysis indicated that hps12 had dwarf plant and shorter pod than wild type（WT）. The cotyledon greening rate of hps12 was decreased after being treated with 4 mmol/L H_2O_2. Furthermore, the isolated leaves of hps12 mutants showed more obvious symptoms of senescence than WT under the treatment of 10 mmol/L H_2O_2. Genetic analysis suggested that hps12 was a monogenic recessive mutant.
文摘已知许多蛋白激酶及蛋白磷酸酶参与了ABA诱导的气孔关闭信号转导过程,而H_2O_2是ABA信号转导链的下游信号成分。运用表皮条生物学分析和激光共聚焦扫描技术,研究促细胞分裂原活化蛋白激酶(mitogen-activated protein kinase,MAPK)对蚕豆保卫细胞中ABA诱导H_2O_2产生的调节作用。用MEK1/2专一抑制剂(PD98059)处理蚕豆叶片的下表皮,抑制了ABA诱导保卫细胞内H_2O_2的产生和气孔关闭。ABA和H_2O_2诱导气孔关闭后,再用PD98059处理,可以使关闭的气孔重新开放,与之相对应的是PD98059使ABA诱导的、已增强了的H_2O_2探针荧光强度降低。而且PD98059不能阻断水杨酸诱导的H_2O_2的产生及其气孔的关闭,因此,MEH1/2调节ABA诱导保卫细胞中H_2O_2产生和积累具有专一性。总之,PD98059通过抑制ABA诱导的H_2O_2的产生并清除其积累而阻断了ABA诱导的气孔关闭。因此,MAPK的激活在保卫细胞H_2O_2信号的产生、放大及其专一性应答信号刺激的反应中有着重要的调节作用。
