The article studied the resonance light scattering of protein red alizarin with the simple fluorescence spectrophotometer first.In Britton Robinson butter of 4.10,the red alizarin combined with proteins by intermolecu...The article studied the resonance light scattering of protein red alizarin with the simple fluorescence spectrophotometer first.In Britton Robinson butter of 4.10,the red alizarin combined with proteins by intermolecular forces to form complexes,causing an enhance of resonance light scattering(RLS) at maximum wavelength of about 360nm.Based on the enhancement of resonance light scattering a novel method for the determination of proteins using red alizarin was developed.Though it affected by the limit of the filter of spectrophotometer the experiment used the wavelength of incident light doesn’t lie in the maximum wavelength,the method has better linear range within 0～10.0μg/ml,determination limits being 0.04μg/ml.The method had been used to determine total proteins in human serum samples and with accuracy and torelant to many foreign substance.展开更多
The second speciation of eight trace elements（Zn,Cr,Mg,Mn,Cu,Fe,Ni and Ca）in Stenoloma chusana（L.）Ching were studied.The aqueous solution of the traditional Chinese herbal was separated into the dissolved and part...The second speciation of eight trace elements（Zn,Cr,Mg,Mn,Cu,Fe,Ni and Ca）in Stenoloma chusana（L.）Ching were studied.The aqueous solution of the traditional Chinese herbal was separated into the dissolved and particulate forms by means of 0.45μm membrane filter.The dissolved form was determined as free ions and non-free ions,organic and inorganic,stable and labile forms by means of cation exchange resin,CHCl3 and D-411 Chelating resin.The results showed that the content of eight elements in particulate are far more than that in dissolved.The contents of free ions form of Zn,Ca and Mg are more than 75%.Mn hasn’t any free ions form.Cu、Fe and Ni exist on mostly non-free ions form.The content of free ions form of Cr is 54.23%.Eight elements exist on mostly inorganic forms after extraction of CHCl3.After the separation of D-411 Chelating resin,the content of Zn in labile form is 62.82%.The contents of else elements in stable form are higher than those in labile form and Ni hasn’t any labile form.展开更多
文摘The article studied the resonance light scattering of protein red alizarin with the simple fluorescence spectrophotometer first.In Britton Robinson butter of 4.10,the red alizarin combined with proteins by intermolecular forces to form complexes,causing an enhance of resonance light scattering(RLS) at maximum wavelength of about 360nm.Based on the enhancement of resonance light scattering a novel method for the determination of proteins using red alizarin was developed.Though it affected by the limit of the filter of spectrophotometer the experiment used the wavelength of incident light doesn’t lie in the maximum wavelength,the method has better linear range within 0～10.0μg/ml,determination limits being 0.04μg/ml.The method had been used to determine total proteins in human serum samples and with accuracy and torelant to many foreign substance.
文摘The second speciation of eight trace elements（Zn,Cr,Mg,Mn,Cu,Fe,Ni and Ca）in Stenoloma chusana（L.）Ching were studied.The aqueous solution of the traditional Chinese herbal was separated into the dissolved and particulate forms by means of 0.45μm membrane filter.The dissolved form was determined as free ions and non-free ions,organic and inorganic,stable and labile forms by means of cation exchange resin,CHCl3 and D-411 Chelating resin.The results showed that the content of eight elements in particulate are far more than that in dissolved.The contents of free ions form of Zn,Ca and Mg are more than 75%.Mn hasn’t any free ions form.Cu、Fe and Ni exist on mostly non-free ions form.The content of free ions form of Cr is 54.23%.Eight elements exist on mostly inorganic forms after extraction of CHCl3.After the separation of D-411 Chelating resin,the content of Zn in labile form is 62.82%.The contents of else elements in stable form are higher than those in labile form and Ni hasn’t any labile form.
