采用间歇发酵实验,研究了葡萄糖浓度、接种量、温度、氮源、不同有机底物对发酵产氢产酸细菌新菌种B49(AF48ll48 in EMBL)生物产氢的影响。结果表明,接种量影响B49的产氢;B49生长和产氢适宜温度均为35℃;B49不能利用无机氮源,而有机氮是...采用间歇发酵实验,研究了葡萄糖浓度、接种量、温度、氮源、不同有机底物对发酵产氢产酸细菌新菌种B49(AF48ll48 in EMBL)生物产氢的影响。结果表明,接种量影响B49的产氢;B49生长和产氢适宜温度均为35℃;B49不能利用无机氮源,而有机氮是B49生长、产氢的适宜氮源;葡萄糖是B49发酵产氢的最适宜底物,当浓度为10g/L时,B49的葡萄糖利用率为100%,氢气得率为1.69mol H_2/mol glucose;此外,B49可利用小麦、大豆、玉米、土豆及糖蜜废水和啤酒废水产氢,其中利用糖蜜废水、啤酒废水产氢分别为137.9ml H_2/ g COD和49.9ml H_2/g COD。展开更多
Three parallel lab-scale expanded granular sludge bed(EGSB) reactors were employed to evaluate the start-up process of bio-hydrogen production from molasses wastewater.With other start-up parameters controlled the sam...Three parallel lab-scale expanded granular sludge bed(EGSB) reactors were employed to evaluate the start-up process of bio-hydrogen production from molasses wastewater.With other start-up parameters controlled the same,the effect of inoculating sludge pre-treatments was studied.Two methods of pre-treatments(pre-heating and pre-aeration) were conducted for the inoculating sludge in two reactors compared with a non-pre-treatment reactor(control reactor).The results showed that the reactors with pre-treatment performed better in the start-up process than the control one.The hydrogen-producing bacteria were efficiently enriched at the start-up period,shortening the start-up process.At the end of the start-up stage,the acidification rate of the pre-treatment reactors was 10%—30% more than the control one,and hydrogen production increased by 1.69—1.82 times.Considering cost and feasibility,the authors suggest applying the pre-aeration method to treat the inoculating sludge to get more efficient start-up in the industrial scale bio-hydrogen production plant.展开更多
文摘采用间歇发酵实验,研究了葡萄糖浓度、接种量、温度、氮源、不同有机底物对发酵产氢产酸细菌新菌种B49(AF48ll48 in EMBL)生物产氢的影响。结果表明,接种量影响B49的产氢;B49生长和产氢适宜温度均为35℃;B49不能利用无机氮源,而有机氮是B49生长、产氢的适宜氮源;葡萄糖是B49发酵产氢的最适宜底物,当浓度为10g/L时,B49的葡萄糖利用率为100%,氢气得率为1.69mol H_2/mol glucose;此外,B49可利用小麦、大豆、玉米、土豆及糖蜜废水和啤酒废水产氢,其中利用糖蜜废水、啤酒废水产氢分别为137.9ml H_2/ g COD和49.9ml H_2/g COD。
文摘Three parallel lab-scale expanded granular sludge bed(EGSB) reactors were employed to evaluate the start-up process of bio-hydrogen production from molasses wastewater.With other start-up parameters controlled the same,the effect of inoculating sludge pre-treatments was studied.Two methods of pre-treatments(pre-heating and pre-aeration) were conducted for the inoculating sludge in two reactors compared with a non-pre-treatment reactor(control reactor).The results showed that the reactors with pre-treatment performed better in the start-up process than the control one.The hydrogen-producing bacteria were efficiently enriched at the start-up period,shortening the start-up process.At the end of the start-up stage,the acidification rate of the pre-treatment reactors was 10%—30% more than the control one,and hydrogen production increased by 1.69—1.82 times.Considering cost and feasibility,the authors suggest applying the pre-aeration method to treat the inoculating sludge to get more efficient start-up in the industrial scale bio-hydrogen production plant.
