背景：在静电纺过程中利用磁场效应的方法更适合制备载有磁性纳米粒子的取向纤维。但当前的研究多限于水溶性聚合物材料的电纺。目的：通过相转移方法制备均匀分散有Fe3O4磁性纳米粒子的聚（左旋乳酸-己内酯）溶液，在外加磁场作用下通...背景：在静电纺过程中利用磁场效应的方法更适合制备载有磁性纳米粒子的取向纤维。但当前的研究多限于水溶性聚合物材料的电纺。目的：通过相转移方法制备均匀分散有Fe3O4磁性纳米粒子的聚（左旋乳酸-己内酯）溶液，在外加磁场作用下通过静电纺技术制备聚（左旋乳酸-己内酯）／Fe3O4定向排列复合纤维，体外接种猪髋动脉内皮细胞，初步观察细胞相容性。设计、时间及地点：对比观察实验，于2008—06／2008—10在东华大学化学化工与生物工程学院生物科学研究所生物材料与组织工程实验室完成。材料：聚（左旋乳酸己内酯）无规共聚物（50：50，Mw30-40万）由日本Nara Medical University提供；猪髋动脉内皮细胞由中国科学院细胞所提供。方法：采用相转移法将水相中的Fe3O4磁性纳米粒子转移至有机溶剂中，制备聚（左旋乳酸己内酯），Fe3O4的溶液，利用磁场对Fe3O4磁性纳米粒子的牵引作用，静电纺制备取向超细纤维。主要观察指标：通过扫描电镜对纤维进行表面形态、取向度进行分析，采用透射电镜分析Fe3O4磁性纳米粒子在纤维中分散和分布情况。在复合Fe3O4磁性纳米粒子的聚（左旋乳酸-己内酯）静电纺纤维膜上体外接种猪髋动脉内皮细胞，采用MTT法检测纤维膜上细胞的黏附和增殖能力以评价其生物相容性。结果：通过添加乳化剂油酸钠成功得到分散均匀的Fe3O4磁性纳米粒子三氯甲烷溶剂。扫描电镜结果表明在磁场的影响下，静电纺得到的纤维表面光滑、无明显珠状物分布；排列方向沿磁场磁力线分布，取向度良好。透射电镜结果显示Fe3O4磁性纳米粒子在静电纺超细纤维中分散度良好。细胞生物相容性结果显示制备的载有磁性纳米粒子的聚（左旋乳酸-己内酯）静电纺超细纤维细胞黏附率优于纯聚（左旋乳酸-己内酯）纤维；其细胞增殖率与纯聚（左旋乳酸-己内酯）静电纺超细纤维接近。结论：含有一定浓度的Fe3O4磁性纳米粒子的聚（左旋乳酸-己内酯）溶液，在外加磁场的作用下，静电纺得到的纤维排列方向沿磁场磁力线分布，取向度极佳，且具有良好的细胞相容性。展开更多
Gambogic acid(GA) is a natural substance with a good antitumor effect, but it is too lipophilic to be metabolized and excreted, thus accumulating in the body. Gemcitabine(GEM), one of the first-line antitumor drugs, h...Gambogic acid(GA) is a natural substance with a good antitumor effect, but it is too lipophilic to be metabolized and excreted, thus accumulating in the body. Gemcitabine(GEM), one of the first-line antitumor drugs, has high hydrophilicity, which greatly shortens its half-life in vivo. We previously reported a compound named N-gamboyl gemcitabine(GAG), derived from the condensation of GEM and GA, whose hydrophilicity is better than GA and stability is better than GEM. Here, the antitumor performance of GAG was investigated for the first time by using several common tumor cell lines as tumor models. The results of in vitro study showed that GAG significantly inhibited the proliferation and migration of the tumor cells. The IC50 values of GAG for the tumor cells were lower than those of GEM and GA. The present study suggests that GAG has a promising potential to be developed into a broad-spectrum antitumor drug.展开更多
文摘背景：在静电纺过程中利用磁场效应的方法更适合制备载有磁性纳米粒子的取向纤维。但当前的研究多限于水溶性聚合物材料的电纺。目的：通过相转移方法制备均匀分散有Fe3O4磁性纳米粒子的聚（左旋乳酸-己内酯）溶液，在外加磁场作用下通过静电纺技术制备聚（左旋乳酸-己内酯）／Fe3O4定向排列复合纤维，体外接种猪髋动脉内皮细胞，初步观察细胞相容性。设计、时间及地点：对比观察实验，于2008—06／2008—10在东华大学化学化工与生物工程学院生物科学研究所生物材料与组织工程实验室完成。材料：聚（左旋乳酸己内酯）无规共聚物（50：50，Mw30-40万）由日本Nara Medical University提供；猪髋动脉内皮细胞由中国科学院细胞所提供。方法：采用相转移法将水相中的Fe3O4磁性纳米粒子转移至有机溶剂中，制备聚（左旋乳酸己内酯），Fe3O4的溶液，利用磁场对Fe3O4磁性纳米粒子的牵引作用，静电纺制备取向超细纤维。主要观察指标：通过扫描电镜对纤维进行表面形态、取向度进行分析，采用透射电镜分析Fe3O4磁性纳米粒子在纤维中分散和分布情况。在复合Fe3O4磁性纳米粒子的聚（左旋乳酸-己内酯）静电纺纤维膜上体外接种猪髋动脉内皮细胞，采用MTT法检测纤维膜上细胞的黏附和增殖能力以评价其生物相容性。结果：通过添加乳化剂油酸钠成功得到分散均匀的Fe3O4磁性纳米粒子三氯甲烷溶剂。扫描电镜结果表明在磁场的影响下，静电纺得到的纤维表面光滑、无明显珠状物分布；排列方向沿磁场磁力线分布，取向度良好。透射电镜结果显示Fe3O4磁性纳米粒子在静电纺超细纤维中分散度良好。细胞生物相容性结果显示制备的载有磁性纳米粒子的聚（左旋乳酸-己内酯）静电纺超细纤维细胞黏附率优于纯聚（左旋乳酸-己内酯）纤维；其细胞增殖率与纯聚（左旋乳酸-己内酯）静电纺超细纤维接近。结论：含有一定浓度的Fe3O4磁性纳米粒子的聚（左旋乳酸-己内酯）溶液，在外加磁场的作用下，静电纺得到的纤维排列方向沿磁场磁力线分布，取向度极佳，且具有良好的细胞相容性。
基金Science&Technology Commission of Shanghai MunicipalityChina (No.20DZ2254900)+3 种基金Municipal Public Welfare Research Project from JiaxingZhejiang ProvinceChina (No.2022AY10001)Open Project Program of Jiaxing Key Laboratory of Virus-Related Infectious Diseases。
文摘Gambogic acid(GA) is a natural substance with a good antitumor effect, but it is too lipophilic to be metabolized and excreted, thus accumulating in the body. Gemcitabine(GEM), one of the first-line antitumor drugs, has high hydrophilicity, which greatly shortens its half-life in vivo. We previously reported a compound named N-gamboyl gemcitabine(GAG), derived from the condensation of GEM and GA, whose hydrophilicity is better than GA and stability is better than GEM. Here, the antitumor performance of GAG was investigated for the first time by using several common tumor cell lines as tumor models. The results of in vitro study showed that GAG significantly inhibited the proliferation and migration of the tumor cells. The IC50 values of GAG for the tumor cells were lower than those of GEM and GA. The present study suggests that GAG has a promising potential to be developed into a broad-spectrum antitumor drug.
