Liver-tissue engineering has proven valuable in treating liver diseases,but the construction of liver tissues with high fidelity remains challenging.Here,we present a novel three-dimensional(3D)-imprinted cell-sheet s...Liver-tissue engineering has proven valuable in treating liver diseases,but the construction of liver tissues with high fidelity remains challenging.Here,we present a novel three-dimensional(3D)-imprinted cell-sheet strategy for the synchronous construction of biomimetic hepatic microtissues with high accuracy in terms of cell type,density,and distribution.To achieve this,the specific composition of hepatic cells in a normal human liver was determined using a spatial proteogenomics dataset.The data and biomimetic hepatic micro-tissues with hexagonal hollow cross-sections indicate that cell information was successfully generated using a homemade 3D-imprinted device for layer-by-layer imprinting and assembling the hepatic cell sheets.By infiltrating vascular endothelial cells into the hollow section of the assembly,biomimetic hepatic microtissues with vascularized channels for nutrient diffusion and drug perfusion can be obtained.We demonstrate that the resultant vascularized biomimetic hepatic micro-tissues can not only be integrated into a microfluidic drug-screening liver-on-a-chip but also assembled into an enlarged physiological structure to promote liver regeneration.We believe that our 3D-imprinted cell sheets strategy will open new avenues for biomimetic microtissue construction.展开更多
To fulfill the long-held dream of human beings to stay health and extend life,regenerative medicine has merged as the future medical direction.Generally,the regenerative medicine aims to repairing or replacing the dys...To fulfill the long-held dream of human beings to stay health and extend life,regenerative medicine has merged as the future medical direction.Generally,the regenerative medicine aims to repairing or replacing the dysfunctional organs caused by diseases or aging[1].The phenomenon of regeneration is widespread among natural species[2].Many animals are born with the ability to repair severely defected tissues or regenerate partially damaged organs.For example,geckoes can grow back their severed tails without leaving any scars;earthworms are able to regenerate new bodies on the cut surface after being chopped.展开更多
基金supported by the National Natural Science Foundation of China(T2225003,and 82100664)the National Key Research and Development Program of China(2022YFA1105300)+4 种基金the Jiangsu Provincial Science and Technology Special Fund for Outstanding Young Scholars(BK20230051)the Nanjing Health Science and Technology Development Project for Distinguished Young Scholars(JQX22003)the Nanjing Medical Science and Technique Development Foundation(ZKX21019)the Clinical Trials from Nanjing Drum Tower Hospital(2022-LCYJ-ZD-01,and 2021-LCYJ-PY-46)the Guangdong Basic and Applied Basic Research Foundation(2021B1515120054).
文摘Liver-tissue engineering has proven valuable in treating liver diseases,but the construction of liver tissues with high fidelity remains challenging.Here,we present a novel three-dimensional(3D)-imprinted cell-sheet strategy for the synchronous construction of biomimetic hepatic microtissues with high accuracy in terms of cell type,density,and distribution.To achieve this,the specific composition of hepatic cells in a normal human liver was determined using a spatial proteogenomics dataset.The data and biomimetic hepatic micro-tissues with hexagonal hollow cross-sections indicate that cell information was successfully generated using a homemade 3D-imprinted device for layer-by-layer imprinting and assembling the hepatic cell sheets.By infiltrating vascular endothelial cells into the hollow section of the assembly,biomimetic hepatic microtissues with vascularized channels for nutrient diffusion and drug perfusion can be obtained.We demonstrate that the resultant vascularized biomimetic hepatic micro-tissues can not only be integrated into a microfluidic drug-screening liver-on-a-chip but also assembled into an enlarged physiological structure to promote liver regeneration.We believe that our 3D-imprinted cell sheets strategy will open new avenues for biomimetic microtissue construction.
基金supported by the National Natural Science Foundation of China(82100664)the Nanjing Health Science and Technology Development Project for Distinguished Young Scholars(JQX22003)+2 种基金Jiangsu Province Postdoctoral Research Funding Program(2021K116B)funding for Clinical Trials from the Affiliated Drum Tower Hospital,Medical School of Nanjing University(2021-LCYJ-PY-46)the Chen Xiao-ping Foundation for the Development of Science and Technology of Hubei Province,China(CXPJJH122002-019)。
文摘To fulfill the long-held dream of human beings to stay health and extend life,regenerative medicine has merged as the future medical direction.Generally,the regenerative medicine aims to repairing or replacing the dysfunctional organs caused by diseases or aging[1].The phenomenon of regeneration is widespread among natural species[2].Many animals are born with the ability to repair severely defected tissues or regenerate partially damaged organs.For example,geckoes can grow back their severed tails without leaving any scars;earthworms are able to regenerate new bodies on the cut surface after being chopped.
