目的总结颈椎后路单开门椎管扩大成形术治疗颈椎后纵韧带骨化症(ossification of posterior longitud inal ligam ent,OPLL)的临床效果。方法对13例OPLL患者施行颈椎后路单开门椎管扩大成形术。结果本组平均手术时间150(120～180)m in,...目的总结颈椎后路单开门椎管扩大成形术治疗颈椎后纵韧带骨化症(ossification of posterior longitud inal ligam ent,OPLL)的临床效果。方法对13例OPLL患者施行颈椎后路单开门椎管扩大成形术。结果本组平均手术时间150(120～180)m in,术中出血量平均为500(300～800)m l。随访3～18个月,术后15月JOA平均改善率53.35%(18.3%～85.7%),临床症状较术前明显改善。本组2例术后有节段性神经根麻痹,无一例出现脑脊液漏、感染等其它并发症。结论颈椎后路单开门椎管扩大成形术治疗颈椎OPLL效果确切,并发症少。展开更多
This study examined the construction of eukaryotic expression plasmid of human transforming growth factor-β3（hTGF-β3） and its inducing effect on the differentiation of precartilaginous stem cells（PSCs） into chon...This study examined the construction of eukaryotic expression plasmid of human transforming growth factor-β3（hTGF-β3） and its inducing effect on the differentiation of precartilaginous stem cells（PSCs） into chondroblasts.hTGF-β3 gene was amplified by using polymerase chain reaction（PCR） and then inserted into the eukaryotic expression plasmid pcDNA3.1 to construct the eukaryotic expression plasmid pcDNA3.1（＋）-hTGF-β3.Rat PSCs were isolated and purified by employing an immunomagnetic cell sorting system.pcDNA3.1（＋）-hTGF-β3 was transfected into purified PSCs with the use of linear polyamines.The expression of TGF-β3 and cartilage-specific extracellular matrix（ECM） components was detected after transfection by real-time quantitative PCR,ELISA,immunochemistry and Western blotting,respectively.The results showed that the eukaryotic expression plasmid pcDNA3.1（＋）-hTGF-β3 was successfully established as identified by enzyme digestion and DNA sequencing.Real-time quantitative PCR and ELISA revealed that hTGF-β3 was strongly expressed in pcDNA3.1（＋）-hTGF-β3-transfected PSCs.Real-time quantitative PCR,immunochemistry and Western blotting showed that the cartilage-specific ECM markers,i.e.,cartilage oligomeric matrix protein（COMP）,Aggrecan,collagen type Ⅹ and Ⅱ were intensely expressed in the pcDNA3.1（＋）-hTGF-β3transfected cells.It was concluded that hTGF-β3 could be stably expressed in pcDNA3.1（＋）-hTGFβ3-transfected PSCs and induce the differentiation of PSCs into chondroblasts.展开更多
文摘目的总结颈椎后路单开门椎管扩大成形术治疗颈椎后纵韧带骨化症(ossification of posterior longitud inal ligam ent,OPLL)的临床效果。方法对13例OPLL患者施行颈椎后路单开门椎管扩大成形术。结果本组平均手术时间150(120～180)m in,术中出血量平均为500(300～800)m l。随访3～18个月,术后15月JOA平均改善率53.35%(18.3%～85.7%),临床症状较术前明显改善。本组2例术后有节段性神经根麻痹,无一例出现脑脊液漏、感染等其它并发症。结论颈椎后路单开门椎管扩大成形术治疗颈椎OPLL效果确切,并发症少。
基金supported by a grant from the National Natural Sciences Foundation of China (No. 30571873)
文摘This study examined the construction of eukaryotic expression plasmid of human transforming growth factor-β3（hTGF-β3） and its inducing effect on the differentiation of precartilaginous stem cells（PSCs） into chondroblasts.hTGF-β3 gene was amplified by using polymerase chain reaction（PCR） and then inserted into the eukaryotic expression plasmid pcDNA3.1 to construct the eukaryotic expression plasmid pcDNA3.1（＋）-hTGF-β3.Rat PSCs were isolated and purified by employing an immunomagnetic cell sorting system.pcDNA3.1（＋）-hTGF-β3 was transfected into purified PSCs with the use of linear polyamines.The expression of TGF-β3 and cartilage-specific extracellular matrix（ECM） components was detected after transfection by real-time quantitative PCR,ELISA,immunochemistry and Western blotting,respectively.The results showed that the eukaryotic expression plasmid pcDNA3.1（＋）-hTGF-β3 was successfully established as identified by enzyme digestion and DNA sequencing.Real-time quantitative PCR and ELISA revealed that hTGF-β3 was strongly expressed in pcDNA3.1（＋）-hTGF-β3-transfected PSCs.Real-time quantitative PCR,immunochemistry and Western blotting showed that the cartilage-specific ECM markers,i.e.,cartilage oligomeric matrix protein（COMP）,Aggrecan,collagen type Ⅹ and Ⅱ were intensely expressed in the pcDNA3.1（＋）-hTGF-β3transfected cells.It was concluded that hTGF-β3 could be stably expressed in pcDNA3.1（＋）-hTGFβ3-transfected PSCs and induce the differentiation of PSCs into chondroblasts.
