目的:本研究拟观测低温常压等离子体射流处理对牙本质-全酸蚀粘接系统粘接效果及牙本质胶原纤维耐NaClO降解的效果。方法:离体牙去除牙合面牙釉质,暴露的牙本质打磨后酸蚀15s并冲洗,随后用NTAPP射流分别处理牙面0、5、10、15、20s,常规...目的:本研究拟观测低温常压等离子体射流处理对牙本质-全酸蚀粘接系统粘接效果及牙本质胶原纤维耐NaClO降解的效果。方法:离体牙去除牙合面牙釉质,暴露的牙本质打磨后酸蚀15s并冲洗,随后用NTAPP射流分别处理牙面0、5、10、15、20s,常规制作微拉伸粘接试件,每组有一半试件冷热循环10 000次,分别检测各组循环前后牙本质粘接强度。获取中层牙本质片,酸蚀后经NTAPP处理0、5、10、15、20s后,分别浸泡于5% NaClO溶液中0、30、60、120s,扫描电镜观察各组脱矿牙本质胶原的降解情况。对各组粘接强度进行统计分析。结果:所有NTAPP处理组的微拉伸粘结强度(Micro-tensile bond strength,μTBS)值均显著高于对照组;NTAPP处理5s组的μTBS值显著高于其他组;老化后NTAPP处理5s和10s组的μTBS值显著高于其他各组。扫描电镜观察脱矿胶原显示,随着NaClO溶液处理时间延长,各组牙本质胶原均呈现不同程度降解,对照组120s胶原完全消失,但NTAPP处理组仍可见胶原纤维残留。NTAPP处理5和10s经NaClO浸泡30s后,胶原纤维网结构完整。NTAPP处理15和20s,使部分胶原纤维凝结变性。结论:采用合适的参数及处理时间,NTAPP可有效保护牙本质胶原纤维并提高牙本质-全酸蚀粘接界面的耐久性。展开更多
In this study, we employed a nonthermal atmospheric pressure plasma(NTAPP) jet to evaluate the effect of plasma treatment on the durability of resin–dentin bonding under a thermocycling challenge. Furthermore, we ass...In this study, we employed a nonthermal atmospheric pressure plasma(NTAPP) jet to evaluate the effect of plasma treatment on the durability of resin–dentin bonding under a thermocycling challenge. Furthermore, we assessed the degradation resistance of plasma-treated collagen under a sodium hypochlorite(NaClO) challenge. We assessed the beneficial effect of NTAPP treatment on the acid-etched dentin–bonding interface by testing the micro-tensile bond strength and examining the morphology. We found that the immediate bonding strength of the dentin significantly increased after NTAPP treatment. Compared with the control group, NTAPP resulted in a more prominent effect on the bonding durability of the dentin–adhesive interface after treatment for 5 or 10 s. Simultaneously, the mechanical strength of dentin collagen under the NaClO challenge was improved. Our results indicate that, in optimal conditions, NTAPP could be a promising method to protect dentin collagen and to improve the bonding durability between dentin and etch-and-rinse adhesives.展开更多
文摘目的:本研究拟观测低温常压等离子体射流处理对牙本质-全酸蚀粘接系统粘接效果及牙本质胶原纤维耐NaClO降解的效果。方法:离体牙去除牙合面牙釉质,暴露的牙本质打磨后酸蚀15s并冲洗,随后用NTAPP射流分别处理牙面0、5、10、15、20s,常规制作微拉伸粘接试件,每组有一半试件冷热循环10 000次,分别检测各组循环前后牙本质粘接强度。获取中层牙本质片,酸蚀后经NTAPP处理0、5、10、15、20s后,分别浸泡于5% NaClO溶液中0、30、60、120s,扫描电镜观察各组脱矿牙本质胶原的降解情况。对各组粘接强度进行统计分析。结果:所有NTAPP处理组的微拉伸粘结强度(Micro-tensile bond strength,μTBS)值均显著高于对照组;NTAPP处理5s组的μTBS值显著高于其他组;老化后NTAPP处理5s和10s组的μTBS值显著高于其他各组。扫描电镜观察脱矿胶原显示,随着NaClO溶液处理时间延长,各组牙本质胶原均呈现不同程度降解,对照组120s胶原完全消失,但NTAPP处理组仍可见胶原纤维残留。NTAPP处理5和10s经NaClO浸泡30s后,胶原纤维网结构完整。NTAPP处理15和20s,使部分胶原纤维凝结变性。结论:采用合适的参数及处理时间,NTAPP可有效保护牙本质胶原纤维并提高牙本质-全酸蚀粘接界面的耐久性。
基金supported by grants from National Natural Science Foundation of China(Nos.81701014,81801310,31700076)the Basic Research of Natural Science Project funded by the Department of Science and Technology of Shaanxi Province(No.2017JM8038)the Science and Technology Project funded by the Science and Technology Bureau of Weiyang District,Xi’an city(No.201846)。
文摘In this study, we employed a nonthermal atmospheric pressure plasma(NTAPP) jet to evaluate the effect of plasma treatment on the durability of resin–dentin bonding under a thermocycling challenge. Furthermore, we assessed the degradation resistance of plasma-treated collagen under a sodium hypochlorite(NaClO) challenge. We assessed the beneficial effect of NTAPP treatment on the acid-etched dentin–bonding interface by testing the micro-tensile bond strength and examining the morphology. We found that the immediate bonding strength of the dentin significantly increased after NTAPP treatment. Compared with the control group, NTAPP resulted in a more prominent effect on the bonding durability of the dentin–adhesive interface after treatment for 5 or 10 s. Simultaneously, the mechanical strength of dentin collagen under the NaClO challenge was improved. Our results indicate that, in optimal conditions, NTAPP could be a promising method to protect dentin collagen and to improve the bonding durability between dentin and etch-and-rinse adhesives.
