目的:观察不同生长阶段秀丽隐杆线虫衰老相关基因的表达变化及川芎提取物(CXE)的调控作用。方法:按生长阶段不同分设2,6 d 2个时间点,采用实时荧光定量RT-PCR(qRT-PCR)方法检测上述时间点对照组和用药组的衰老相关基因表达情况,以2-Δ...目的:观察不同生长阶段秀丽隐杆线虫衰老相关基因的表达变化及川芎提取物(CXE)的调控作用。方法:按生长阶段不同分设2,6 d 2个时间点,采用实时荧光定量RT-PCR(qRT-PCR)方法检测上述时间点对照组和用药组的衰老相关基因表达情况,以2-ΔΔCT法比较分析其基因表达变化的差异及CXE的调控作用。结果:与2 d对照组比较,6 d对照组线虫的age-1,daf-2,let-363基因表达上调(P<0.05),而ins-18,let-60,sir-2.1,sod-3基因表达下调(P<0.05);与用药2 d组比较,用药6 d组age-1,daf-2,let-363基因表达显著下调(P<0.01),ins-18,let-60,sir-2.1,sod-3基因表达显著上调(P<0.01)。结论:age-1,daf-2,let-363基因进入衰老期后表达量增加,显示促进衰老作用;而ins-18,let-60,sir-2.1,sod-3进入衰老期后表达量下降,显示延缓衰老的作用。CXE主要通过抑制胰岛素信号通路和限食等通路的衰老基因表达,促进长寿基因表达发挥延寿作用。展开更多
Objective： To investigate the effects of Erzhi Pill （二至丸,,EZP） on nerve cell apoptosis iinn senescence model rats.Methods： The rats model of senescence was established by peritoneal D-galactose injection combin...Objective： To investigate the effects of Erzhi Pill （二至丸,,EZP） on nerve cell apoptosis iinn senescence model rats.Methods： The rats model of senescence was established by peritoneal D-galactose injection combined with thymusectomy.Forty SD rats were randomized into four groups,the normal control group,the senescence model group,the EZP treated group,and the vitamins treated group,10 in each group.The rats were made into senescence model except those in the normal group.In the same time of D-galactose injection,the rats were treated respectively with distilled water,EZP 4.32 g/kg,and vitamins E and C 0.06 g/kg daily for 6 weeks via intragastric infusion.The index of main viscera （as brain,testis,etc.）,serum levels of superoxide dismutase （SOD） activity,and total anti-oxidation capacity （T-AOC） were measured after a 6-week treatment.Meanwhile,the cerebral cortex neuronal apoptosis proportion and mitochondrial membrane potential （MMP） were detected by flow cytometry.Results： Both EZP and vitamins E and C treatments showed effects on increasing testis index and serum level of T-AOC,reducing the percentage of neuronal apoptosis in the cerebral cortex,and elevating MMP in the aging rats model.Conclusions： EZP could inhibit the cerebral cortex neuron apoptosis and maintain the mitochondrial function in the senescent process of rats induced by peritoneal D-galactose injection combined with thymusectomy.It also shows antioxidation effect to some extents.展开更多
基金Supported by the National Natural Science Foundation of China(No.30901991)the Business Fees of China Academy of Chinese Medical Sciences for Independent Topics of Basic Researches (No.ZZ2006037)
文摘Objective： To investigate the effects of Erzhi Pill （二至丸,,EZP） on nerve cell apoptosis iinn senescence model rats.Methods： The rats model of senescence was established by peritoneal D-galactose injection combined with thymusectomy.Forty SD rats were randomized into four groups,the normal control group,the senescence model group,the EZP treated group,and the vitamins treated group,10 in each group.The rats were made into senescence model except those in the normal group.In the same time of D-galactose injection,the rats were treated respectively with distilled water,EZP 4.32 g/kg,and vitamins E and C 0.06 g/kg daily for 6 weeks via intragastric infusion.The index of main viscera （as brain,testis,etc.）,serum levels of superoxide dismutase （SOD） activity,and total anti-oxidation capacity （T-AOC） were measured after a 6-week treatment.Meanwhile,the cerebral cortex neuronal apoptosis proportion and mitochondrial membrane potential （MMP） were detected by flow cytometry.Results： Both EZP and vitamins E and C treatments showed effects on increasing testis index and serum level of T-AOC,reducing the percentage of neuronal apoptosis in the cerebral cortex,and elevating MMP in the aging rats model.Conclusions： EZP could inhibit the cerebral cortex neuron apoptosis and maintain the mitochondrial function in the senescent process of rats induced by peritoneal D-galactose injection combined with thymusectomy.It also shows antioxidation effect to some extents.
