本文用 ABC 免疫组方法研究了 HI30、HI100,HI161及 HI98四种单抗所识别抗原在正常及病理组织中的分布特点,这四个单抗既可在冰冻切片也可在石蜡切片上检测胞膜及胞浆抗原。HI30可区别造血与非造血肿瘤,HI100可识别 B 细胞而 HI161可识...本文用 ABC 免疫组方法研究了 HI30、HI100,HI161及 HI98四种单抗所识别抗原在正常及病理组织中的分布特点,这四个单抗既可在冰冻切片也可在石蜡切片上检测胞膜及胞浆抗原。HI30可区别造血与非造血肿瘤,HI100可识别 B 细胞而 HI161可识别 T 细胞肿瘤,H198可区别粒细胞与淋巴细胞肿瘤。四种单抗联合应用可鉴别造血与非造血,淋巴与粒系以及 T 细胞与 B 细胞来源的肿瘤。展开更多
An in vitro-vivo technique for establishment of cell lines on murine leukemia has beendeveloped. Using this method, suppressive T lymphoblastic leukemia L7811-85, L7212-85, non-T, non-B lymphocytic leukemia L1210-86, ...An in vitro-vivo technique for establishment of cell lines on murine leukemia has beendeveloped. Using this method, suppressive T lymphoblastic leukemia L7811-85, L7212-85, non-T, non-B lymphocytic leukemia L1210-86, B lymphocytic leukemia P 388-86 and Friend erythroleu-kemia FLCL cell lines have been established. Incidence of leukemia with these cell lines was 100%. Along with the increase of genera-tions of cell lines, cell growth accelerated, generation time shortened and cloning efficienciesrose. A following up electron microscopic observation on L7811-85 and L7212-85 showed thatthe virus particles were "A" particles in original cells. When they became cell lines in vitro,virus particles increased and transformed into typical "C" particles with budding. An inhibitory activity relevant to leukemic cells on proliferation of leukemic cells hasbeen observed in the supernatant of L7811-85 medium and was regarded as an "autocrine".展开更多
文摘本文用 ABC 免疫组方法研究了 HI30、HI100,HI161及 HI98四种单抗所识别抗原在正常及病理组织中的分布特点,这四个单抗既可在冰冻切片也可在石蜡切片上检测胞膜及胞浆抗原。HI30可区别造血与非造血肿瘤,HI100可识别 B 细胞而 HI161可识别 T 细胞肿瘤,H198可区别粒细胞与淋巴细胞肿瘤。四种单抗联合应用可鉴别造血与非造血,淋巴与粒系以及 T 细胞与 B 细胞来源的肿瘤。
基金the National Natural Science Foundation of China.
文摘An in vitro-vivo technique for establishment of cell lines on murine leukemia has beendeveloped. Using this method, suppressive T lymphoblastic leukemia L7811-85, L7212-85, non-T, non-B lymphocytic leukemia L1210-86, B lymphocytic leukemia P 388-86 and Friend erythroleu-kemia FLCL cell lines have been established. Incidence of leukemia with these cell lines was 100%. Along with the increase of genera-tions of cell lines, cell growth accelerated, generation time shortened and cloning efficienciesrose. A following up electron microscopic observation on L7811-85 and L7212-85 showed thatthe virus particles were "A" particles in original cells. When they became cell lines in vitro,virus particles increased and transformed into typical "C" particles with budding. An inhibitory activity relevant to leukemic cells on proliferation of leukemic cells hasbeen observed in the supernatant of L7811-85 medium and was regarded as an "autocrine".
