A porous NiO/yttria-stabilized zirconia was prepared by gel casting technique. anode substrate for tubular solid oxide fuel cells Nano-scale samaria-doped ceria （SDC） particles were formed onto the anode substrate t...A porous NiO/yttria-stabilized zirconia was prepared by gel casting technique. anode substrate for tubular solid oxide fuel cells Nano-scale samaria-doped ceria （SDC） particles were formed onto the anode substrate to modify the anode microstructure by the impregnation of solution of Sm（NO3）3 and Ce（NO3）3. Electrochemical impedance spectroscopy, current-voltage and current-powder curves of the cells were measured using an electrochemical workstation. Scanning electron microcopy was used to observe the microstructure. The results indicate that the stability of the performance of the cell operated on humidified methane can be significantly improved by incorporating the nano-structured SDC particles, compared with the unmodified cell. This verifies that the coated SDC electrodes are very effective in suppressing catalytic carbon formation by blocking methane from approaching the Ni, which is catalytically active towards methane pyrolysis. In addition, it was found that a small amount of deposited carbon is beneficial to the performance of the anode. The cell showed a peak power density of 225 mW/cm^2 when it was fed with H2 fuel at 700 ℃, but the power density increased to 400 mW/cm^2 when the fuel was switched from hydrogen to methane at the same flow rate. Methane conversion achieved about 90%, measured by gas chromatogram with a 10.0 mL/min flow rate of fuel at 700 ℃. Although the carbon deposition was not suppressed absolutely, some deposited carbon was beneficial for performance improvement.展开更多
目的应用蛋白质芯片技术检测早中期和晚期肺癌患者血清中的差异表达蛋白并探讨其临床意义。方法应用表面增强激光解析电离飞行时间质谱（SELDI—TOF—Ms）技术，使用弱阳离子交换（WCX-2）蛋白芯片分别检测两组肺癌患者血清蛋白指纹图...目的应用蛋白质芯片技术检测早中期和晚期肺癌患者血清中的差异表达蛋白并探讨其临床意义。方法应用表面增强激光解析电离飞行时间质谱（SELDI—TOF—Ms）技术，使用弱阳离子交换（WCX-2）蛋白芯片分别检测两组肺癌患者血清蛋白指纹图谱，采用蛋白质芯片阅读机读取数据，Ciphergen Protein Chip3．0版本的分析软件采集数据，读取峰信息，进行统计学处理。两组之间蛋白质峰强度的比较采用x。检验。结果早中期和晚期肺癌患者血清中存在10个有统计学意义的差异蛋白质峰。与早中期相比，晚期肺癌患者血清中高表达的蛋白质相对分子质量为7978、8139、15951和16133，晚期肺癌患者血清中低表达的蛋白质相对分子质量为2867、6885、8701、8840、13781、13955。结论SELDI—TOF—MS蛋白质芯片技术是一种快速、简单、敏感、样本用量少和高通量的蛋白质检查分析方法，可以直接筛选早中期和晚期肺癌患者血清中的差异蛋白，可能成为远处转移的诊断指标，具有较好的临床应用价值。展开更多
基金ACKNOWLEDGMENTS This work was supported by the National Natural Science Foundation of China （No.20871110 and No.50730002）. The authors express their appreciation to Xin-bo Lii, Qingdao Tianhe Graphite Co. Ltd. for supporting appropriate pore former graphite.
文摘A porous NiO/yttria-stabilized zirconia was prepared by gel casting technique. anode substrate for tubular solid oxide fuel cells Nano-scale samaria-doped ceria （SDC） particles were formed onto the anode substrate to modify the anode microstructure by the impregnation of solution of Sm（NO3）3 and Ce（NO3）3. Electrochemical impedance spectroscopy, current-voltage and current-powder curves of the cells were measured using an electrochemical workstation. Scanning electron microcopy was used to observe the microstructure. The results indicate that the stability of the performance of the cell operated on humidified methane can be significantly improved by incorporating the nano-structured SDC particles, compared with the unmodified cell. This verifies that the coated SDC electrodes are very effective in suppressing catalytic carbon formation by blocking methane from approaching the Ni, which is catalytically active towards methane pyrolysis. In addition, it was found that a small amount of deposited carbon is beneficial to the performance of the anode. The cell showed a peak power density of 225 mW/cm^2 when it was fed with H2 fuel at 700 ℃, but the power density increased to 400 mW/cm^2 when the fuel was switched from hydrogen to methane at the same flow rate. Methane conversion achieved about 90%, measured by gas chromatogram with a 10.0 mL/min flow rate of fuel at 700 ℃. Although the carbon deposition was not suppressed absolutely, some deposited carbon was beneficial for performance improvement.
文摘目的应用蛋白质芯片技术检测早中期和晚期肺癌患者血清中的差异表达蛋白并探讨其临床意义。方法应用表面增强激光解析电离飞行时间质谱（SELDI—TOF—Ms）技术，使用弱阳离子交换（WCX-2）蛋白芯片分别检测两组肺癌患者血清蛋白指纹图谱，采用蛋白质芯片阅读机读取数据，Ciphergen Protein Chip3．0版本的分析软件采集数据，读取峰信息，进行统计学处理。两组之间蛋白质峰强度的比较采用x。检验。结果早中期和晚期肺癌患者血清中存在10个有统计学意义的差异蛋白质峰。与早中期相比，晚期肺癌患者血清中高表达的蛋白质相对分子质量为7978、8139、15951和16133，晚期肺癌患者血清中低表达的蛋白质相对分子质量为2867、6885、8701、8840、13781、13955。结论SELDI—TOF—MS蛋白质芯片技术是一种快速、简单、敏感、样本用量少和高通量的蛋白质检查分析方法，可以直接筛选早中期和晚期肺癌患者血清中的差异蛋白，可能成为远处转移的诊断指标，具有较好的临床应用价值。
