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恙虫病立克次体组织培养 Ⅱ.应用兔睾丸单层细胞培养恙虫病立克次体
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作者 冯慧敏 罗瑞仙 白施恩 《微生物学报》 CAS 1963年第3期280-283,共4页
近年来,组织培养技术进展很快,自Dulbecco,Youngner等氏(1952,1954)以胰酶消化组织获得单层细胞,培养脊髓灰质炎病毒获得成功以来,许多学者都证实多种病毒能于各种单层细胞培养中繁殖,并引起细胞病变,便于观察病毒的繁殖。故目前单层细... 近年来,组织培养技术进展很快,自Dulbecco,Youngner等氏(1952,1954)以胰酶消化组织获得单层细胞,培养脊髓灰质炎病毒获得成功以来,许多学者都证实多种病毒能于各种单层细胞培养中繁殖,并引起细胞病变,便于观察病毒的繁殖。故目前单层细胞培养技术不但广泛应用于病毒分离培养及鉴定,同时也是研究病毒与宿主细胞间关系及病毒变异等问题的重要工具之一。立克次体一般繁殖较慢,除Q热立克次体外。 展开更多
关键词 立克次体 单层 涂片 培养管 胞中 立克次氏体 恙虫病 立克次氏体病 小白鼠
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HISTOCHEMICAL CHANGES OF HOST CELLS IN TISSUE CULTURE INOCULATED WITH RICKETTSIA TSUTSUGAMUSHI
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作者 冯慧敏 欧阳祥林 白施恩 《Chinese Science Bulletin》 SCIE EI CAS 1966年第7期311-312,共2页
Investigation of metabolic changes of host cells in tissue culture caused by Rickettsia tsutsugamushi helps to elucidate the pathogenesis and mechanism of immunity in scrub typhus. We used the histochemical methods fo... Investigation of metabolic changes of host cells in tissue culture caused by Rickettsia tsutsugamushi helps to elucidate the pathogenesis and mechanism of immunity in scrub typhus. We used the histochemical methods for observing the changes of nucleic acids in the rabbit testicular monolayer tissue culture inoculated with R. tsutsugamushi. The method of preparation and inoculation of the tissue culture was the same as already reported. In the present experiment a glass slide was first inserted into the tube. before introduction of tissue cells. Examination was made once every 4 days after the cells had been inoculated with rickettsia. On the days 展开更多
关键词 immunity inserted nucleic RABBIT observing PATHOGENESIS HISTOCHEMICAL elucidate SLIDE inoculation
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