This study examined the effect of different b values on diffusion-weighted MR imaging （DWI） of human prostate by using single-shot spin echo echo planar imaging （SE-EPI） sequences, observed the normal appearances ...This study examined the effect of different b values on diffusion-weighted MR imaging （DWI） of human prostate by using single-shot spin echo echo planar imaging （SE-EPI） sequences, observed the normal appearances and measured apparent diffusion coefficient （ADC） values in anatomical regions of normal prostate. Twenty-four healthy volunteers （mean age： 32 y） were studied by using a 1.5T system with a phased array surface multicoil. Two kinds of single-shot SE-EPI sequence were used to perform DWI in the prostate in volunteers, with five b values being 0, 30, 300, 500 to 1000 s/mm2. The image quality with different imaging parameters was analyzed and the ADC values in anatomical regions of normal prostate were measured. DWI of prostate was successfully obtained in all volunteers. The images were of good quality, without artifacts containing pixels within the prostate. The contrast was good between the different anatomical regions of the prostatic gland, i.e., the peripheral zone （PZ）, which exhibited higher signal intensity, and the central gland （CG）. Signal intensity contrast was related to the magnitude of b values. The ADC values in PZ and CG were （1.27±0.22）×10-3 mm2/s and （1.01±0.17）×10-3 mm2/s,respectively. The ADC values were found to be significantly higher in PZ than in CG （P〈0.05, paired t-test）. Significant differences were found between the slice-selecting component and both the read-out and phase-encoding components of the ADC values. It is concluded that SE-EPI is a suitable DWI sequence for human prostate. The contrast between PZ and CG is good when b values are low, while the diffusion and ADC values are accurate when b values are high. ADC values are higher in PZ than in CG in normal prostate. Diffusional anisotropy is present in normal prostatic tissue.展开更多
The MRI appearances of 7 adult patients with pathologically proven intracranial primitive neuroectodermal tumors (PNET) were retrospectively analyzed. The MRI features were compared with findings in pathology and surg...The MRI appearances of 7 adult patients with pathologically proven intracranial primitive neuroectodermal tumors (PNET) were retrospectively analyzed. The MRI features were compared with findings in pathology and surgery. In this group, the tumor masses were most commonly found in the semisphere of cerebrum and in the vermis of cerebellum. They were relatively large and 4 were in lobulated shape. All of them had well defined margins. MR images showed the tumors to be mildly or obviously hypointense on T 1 weighted images and hyperintense on T 2 weighted images. Most masses had heterogeneous appearances with some cystic and necrotic areas. Intratumoral haemorrhage and focal calcification were occasionally seen. Mostly, there was no or only mild surrounding edema. Marked inhomogeneous contrast enhancement on MRI was seen in 6 cases except one. Two patients with multiple intracranial metastases were revealed on MR images. In this series, there was good correlation between MRI features and findings in pathology and surgery. These results showed that certain MRI features might suggest the diagnosis of intracranial PNET in adults. MRI is an effective technique to detect these tumors and is helpful to treatment planning and follow up.展开更多
This study developed a novel approach of targeting malignant glioma with pMAGE-A1278-286-specific cytotoxic T lymphocytes (CTLs) induced from the peripheral blood mononuclear cells of healthy donors by multiple stimul...This study developed a novel approach of targeting malignant glioma with pMAGE-A1278-286-specific cytotoxic T lymphocytes (CTLs) induced from the peripheral blood mononuclear cells of healthy donors by multiple stimulations with human leukocyte antigen (HLA)-A2-restricted pMAGE-A1278-286 peptide-pulsed dentritic cells.Cytotoxic assays were performed by the colorimetric CytoTox 96 assay to analyze cytotoxic activity of the induced CTLs against various target cells.The induced CTLs showed approximately 45% specific lysis against T2pMAGE-A1278-286 (pMAGE-A1278-286 peptide pulsed T2 cells) and U251 (HLA-A2+, MAGE-A1+) at an effector:target ratio of 40:1, and approximately 5% cytolysis against T2pHIV, A172 (HLA-A2-, MAGE-A1+), K562 and T2 cells without being pulsed with peptide at any effector:target ratio.The specific killing activity of the induced CTLs against T2pMAGE-A1278-286 and U251 was much more obvious than in any other control group (P【0.05).The cytotoxic activity against the T2pMAGE-A1278-286 and U251 was significantly eliminated by anti-HLA class Ⅰ mAb W6/32.These results suggest that pMAGE-A1278-286 epitope may serve as a surrogate tumor antigen target of specific immunotherapy for treating HLA-A2 patients with malignant glioma.展开更多
三维稳态自由进动序列 （ three dimensional-steady state free precession,3D-SSFP） MRI 是对心脏大血管的自由呼吸、导航门控、心电触发、各向同性的三维容积扫描，使得MRI对心脏大血管的显影在传统无创、无射线的基础上进一步实...三维稳态自由进动序列 （ three dimensional-steady state free precession,3D-SSFP） MRI 是对心脏大血管的自由呼吸、导航门控、心电触发、各向同性的三维容积扫描，使得MRI对心脏大血管的显影在传统无创、无射线的基础上进一步实现了无需屏气及无对比剂显影，可综合评估心脏、大血管及冠状动脉异常。笔者对2008年8月至2010年2月临床怀疑有心脏大血管异常的患者进行了胸腔3D—SSFP扫描及传统心血管MR扫描（CMR），展开更多
基金supported by a grant from Key Project of Science and Technology Research of Hubei Province of China (No. 2005AA304B08).
文摘This study examined the effect of different b values on diffusion-weighted MR imaging （DWI） of human prostate by using single-shot spin echo echo planar imaging （SE-EPI） sequences, observed the normal appearances and measured apparent diffusion coefficient （ADC） values in anatomical regions of normal prostate. Twenty-four healthy volunteers （mean age： 32 y） were studied by using a 1.5T system with a phased array surface multicoil. Two kinds of single-shot SE-EPI sequence were used to perform DWI in the prostate in volunteers, with five b values being 0, 30, 300, 500 to 1000 s/mm2. The image quality with different imaging parameters was analyzed and the ADC values in anatomical regions of normal prostate were measured. DWI of prostate was successfully obtained in all volunteers. The images were of good quality, without artifacts containing pixels within the prostate. The contrast was good between the different anatomical regions of the prostatic gland, i.e., the peripheral zone （PZ）, which exhibited higher signal intensity, and the central gland （CG）. Signal intensity contrast was related to the magnitude of b values. The ADC values in PZ and CG were （1.27±0.22）×10-3 mm2/s and （1.01±0.17）×10-3 mm2/s,respectively. The ADC values were found to be significantly higher in PZ than in CG （P〈0.05, paired t-test）. Significant differences were found between the slice-selecting component and both the read-out and phase-encoding components of the ADC values. It is concluded that SE-EPI is a suitable DWI sequence for human prostate. The contrast between PZ and CG is good when b values are low, while the diffusion and ADC values are accurate when b values are high. ADC values are higher in PZ than in CG in normal prostate. Diffusional anisotropy is present in normal prostatic tissue.
文摘The MRI appearances of 7 adult patients with pathologically proven intracranial primitive neuroectodermal tumors (PNET) were retrospectively analyzed. The MRI features were compared with findings in pathology and surgery. In this group, the tumor masses were most commonly found in the semisphere of cerebrum and in the vermis of cerebellum. They were relatively large and 4 were in lobulated shape. All of them had well defined margins. MR images showed the tumors to be mildly or obviously hypointense on T 1 weighted images and hyperintense on T 2 weighted images. Most masses had heterogeneous appearances with some cystic and necrotic areas. Intratumoral haemorrhage and focal calcification were occasionally seen. Mostly, there was no or only mild surrounding edema. Marked inhomogeneous contrast enhancement on MRI was seen in 6 cases except one. Two patients with multiple intracranial metastases were revealed on MR images. In this series, there was good correlation between MRI features and findings in pathology and surgery. These results showed that certain MRI features might suggest the diagnosis of intracranial PNET in adults. MRI is an effective technique to detect these tumors and is helpful to treatment planning and follow up.
基金supported by grants from the National Natural Science Foundation of China (No.30801355)Natural Science Foundation of Hubei Province (No.2009CDB060)Program for New Century Excellent Talents in University (NECT-06-0761)
文摘This study developed a novel approach of targeting malignant glioma with pMAGE-A1278-286-specific cytotoxic T lymphocytes (CTLs) induced from the peripheral blood mononuclear cells of healthy donors by multiple stimulations with human leukocyte antigen (HLA)-A2-restricted pMAGE-A1278-286 peptide-pulsed dentritic cells.Cytotoxic assays were performed by the colorimetric CytoTox 96 assay to analyze cytotoxic activity of the induced CTLs against various target cells.The induced CTLs showed approximately 45% specific lysis against T2pMAGE-A1278-286 (pMAGE-A1278-286 peptide pulsed T2 cells) and U251 (HLA-A2+, MAGE-A1+) at an effector:target ratio of 40:1, and approximately 5% cytolysis against T2pHIV, A172 (HLA-A2-, MAGE-A1+), K562 and T2 cells without being pulsed with peptide at any effector:target ratio.The specific killing activity of the induced CTLs against T2pMAGE-A1278-286 and U251 was much more obvious than in any other control group (P【0.05).The cytotoxic activity against the T2pMAGE-A1278-286 and U251 was significantly eliminated by anti-HLA class Ⅰ mAb W6/32.These results suggest that pMAGE-A1278-286 epitope may serve as a surrogate tumor antigen target of specific immunotherapy for treating HLA-A2 patients with malignant glioma.
文摘三维稳态自由进动序列 （ three dimensional-steady state free precession,3D-SSFP） MRI 是对心脏大血管的自由呼吸、导航门控、心电触发、各向同性的三维容积扫描，使得MRI对心脏大血管的显影在传统无创、无射线的基础上进一步实现了无需屏气及无对比剂显影，可综合评估心脏、大血管及冠状动脉异常。笔者对2008年8月至2010年2月临床怀疑有心脏大血管异常的患者进行了胸腔3D—SSFP扫描及传统心血管MR扫描（CMR），
基金the National Natural Science Foundation of China(81974382)the Major Scientific and Technological Innovation Projects in Hubei Province(2018ACA136)COVID-19 Program of Huazhong University of Science and Technology(2020kfyXGYJ099)。
