目的:利用网络药理学去探究鱼腥草治疗急性肺损伤的作用机制。方法:首先,通过TCMSP、Swisws Target Prediction数据库查找鱼腥草的有效成分,并利用Uniport数据库将有效成分转化为基因靶点;然后通过GeneCards、OMIM、DisGeNET数据库获取...目的:利用网络药理学去探究鱼腥草治疗急性肺损伤的作用机制。方法:首先,通过TCMSP、Swisws Target Prediction数据库查找鱼腥草的有效成分,并利用Uniport数据库将有效成分转化为基因靶点;然后通过GeneCards、OMIM、DisGeNET数据库获取急性肺损伤疾病的靶点,再将两者基因相交,筛选得到共同的基因靶点,并用Venny绘图,再使用Cytoscape去构建中药–疾病–成分–靶点的网络图,并从STRING数据库分析并筛选获得相同靶点蛋白–蛋白相互作用(PPI)网络图,利用Cytoscape软件分析绘制PPI网络图,运用cytoHubba的Degree算法,筛选关键基因靶点,建立关键基因靶点的网络模型。同时,使用微生信在线平台去进行富集分析,分析潜在靶点的基因功能以及信号通路,从而从系统生物学整体水平揭示鱼腥草潜在有效成分和作用机制。结果:结果分为三部分:成分、靶点和途径。在成分方面,发现鱼腥草的5种活性成分,其中槲皮素、山奈酚是主要活性成分。共发现132个靶点,其中128个主要靶点和ALI共同拥有。此外,鱼腥草治疗ALI的主要通路是Pathways in cancer信号通路、Lipid and atherosclerosis信号通路、AGE-RAGE信号通路等。结论:由于鱼腥草的多组分、多靶点和多通道功能,本研究通过网络药理学初步揭示了鱼腥草治疗ALI的潜在调节网络。为合理开发利用鱼腥草植物资源、保护和发展民族医药文化提供研究依据。展开更多
A real-time label-free DNA biosensor based on thin-core fiber(TCF) interferometer is demonstrated experimentally. The proposed biosensor is constructed by splicing a TCF between two segments of single mode fibers(SMFs...A real-time label-free DNA biosensor based on thin-core fiber(TCF) interferometer is demonstrated experimentally. The proposed biosensor is constructed by splicing a TCF between two segments of single mode fibers(SMFs) and integrated into a microfluidic channel. By modifying the TCF surface with monolayer poly-l-lysine(PLL) and single-stranded deoxyribonucleic acid(ssDNA) probes, the target DNA molecules can be captured in the microfluidic channel. The transmission spectra of the biosensor are measured and theoretically analyzed under different biosensing reaction processes. The results show that the wavelength has a blue-shift with the process of the DNA hybridization. Due to the advantages of low cost, simple operation as well as good detection effect on DNA molecules hybridization, the proposed biosensor has great application prospects in the fields of gene sequencing, medical diagnosis, cancer detection and environmental engineering.展开更多
文摘目的:利用网络药理学去探究鱼腥草治疗急性肺损伤的作用机制。方法:首先,通过TCMSP、Swisws Target Prediction数据库查找鱼腥草的有效成分,并利用Uniport数据库将有效成分转化为基因靶点;然后通过GeneCards、OMIM、DisGeNET数据库获取急性肺损伤疾病的靶点,再将两者基因相交,筛选得到共同的基因靶点,并用Venny绘图,再使用Cytoscape去构建中药–疾病–成分–靶点的网络图,并从STRING数据库分析并筛选获得相同靶点蛋白–蛋白相互作用(PPI)网络图,利用Cytoscape软件分析绘制PPI网络图,运用cytoHubba的Degree算法,筛选关键基因靶点,建立关键基因靶点的网络模型。同时,使用微生信在线平台去进行富集分析,分析潜在靶点的基因功能以及信号通路,从而从系统生物学整体水平揭示鱼腥草潜在有效成分和作用机制。结果:结果分为三部分:成分、靶点和途径。在成分方面,发现鱼腥草的5种活性成分,其中槲皮素、山奈酚是主要活性成分。共发现132个靶点,其中128个主要靶点和ALI共同拥有。此外,鱼腥草治疗ALI的主要通路是Pathways in cancer信号通路、Lipid and atherosclerosis信号通路、AGE-RAGE信号通路等。结论:由于鱼腥草的多组分、多靶点和多通道功能,本研究通过网络药理学初步揭示了鱼腥草治疗ALI的潜在调节网络。为合理开发利用鱼腥草植物资源、保护和发展民族医药文化提供研究依据。
基金supported by the National Undergraduate Innovation Training Program(No.201710055065)
文摘A real-time label-free DNA biosensor based on thin-core fiber(TCF) interferometer is demonstrated experimentally. The proposed biosensor is constructed by splicing a TCF between two segments of single mode fibers(SMFs) and integrated into a microfluidic channel. By modifying the TCF surface with monolayer poly-l-lysine(PLL) and single-stranded deoxyribonucleic acid(ssDNA) probes, the target DNA molecules can be captured in the microfluidic channel. The transmission spectra of the biosensor are measured and theoretically analyzed under different biosensing reaction processes. The results show that the wavelength has a blue-shift with the process of the DNA hybridization. Due to the advantages of low cost, simple operation as well as good detection effect on DNA molecules hybridization, the proposed biosensor has great application prospects in the fields of gene sequencing, medical diagnosis, cancer detection and environmental engineering.
