在我国经济持续稳定发展的背景下，国家通过各种研究计划（如973计划、863项目、NSFC等）和国家知识创新体系等形式大力支持具有国家战略需求的基础研究，使植物科学研究飞速发展并受到国际同行的高度重视。体现在我国不少科学家担任国...在我国经济持续稳定发展的背景下，国家通过各种研究计划（如973计划、863项目、NSFC等）和国家知识创新体系等形式大力支持具有国家战略需求的基础研究，使植物科学研究飞速发展并受到国际同行的高度重视。体现在我国不少科学家担任国际学术组织负责人或重要国际期刊编委，如许智宏院士担任国际植物组（The International Association for Plant Tissue Culture and Biotechnology,IAPTC ＆ B；）展开更多
A vernalization-related gene cDNA clone (verc 203 ) from winter wheat was subeloned into restrichon enzyme sites of furnH 1 and Mind Ⅲ in PW19 plashed. The sequence of the cDNA insert was deterdrined using the method...A vernalization-related gene cDNA clone (verc 203 ) from winter wheat was subeloned into restrichon enzyme sites of furnH 1 and Mind Ⅲ in PW19 plashed. The sequence of the cDNA insert was deterdrined using the method of T7 sequencing for double strand DNA. The results showed that verc203 consisted of 195 base Pairs,and had 31 sites of restriction enzymes which were covered by 17 enzymes.Homological analysis of verc 203 and each gene sequence involved in EMBL(European Molecular Biology library )data was done in a computer with FASP method. It was found to be not homologous with verc 203 because the highest score in the 20 genes with highest scores is 42 only, far lower than 50 which is the lowest score as the standard of homology between sequences in the FASP method. On the basis of the results of homologlcal analysis for each gene in EMBL, we conclude that verc 203 may be the cDNA clone of a new gene in higher plants, and its function is related to vernalization in winter wheat.展开更多
Ver203 ,a gene related to vernalization in winter wheat was cloned by the strategy of differential screening and RACE PCR. Its function was investigated by antisense transformation in both wheat and Arabidopsis. A num...Ver203 ,a gene related to vernalization in winter wheat was cloned by the strategy of differential screening and RACE PCR. Its function was investigated by antisense transformation in both wheat and Arabidopsis. A number of transgenic plants were obtained and their response to vernalization treatment was characterized. For the plants in the 6 lines in which the antisense construct was expressed efficiently, flowering stage was significantly delayed. The developmental pattern of inflorescence was also changed in the transgenic plants of wheat and Arabidopsis . The upper spikes of transgenic plants were degenerated in wheat, and the florescence of transgenic plants became terminal flower in Arabidopsis . This suggests that the VER203 type of proteins may play an important role in floral initiation and flower development in winter wheat and Arabidopsis.展开更多
文摘在我国经济持续稳定发展的背景下，国家通过各种研究计划（如973计划、863项目、NSFC等）和国家知识创新体系等形式大力支持具有国家战略需求的基础研究，使植物科学研究飞速发展并受到国际同行的高度重视。体现在我国不少科学家担任国际学术组织负责人或重要国际期刊编委，如许智宏院士担任国际植物组（The International Association for Plant Tissue Culture and Biotechnology,IAPTC ＆ B；）
文摘A vernalization-related gene cDNA clone (verc 203 ) from winter wheat was subeloned into restrichon enzyme sites of furnH 1 and Mind Ⅲ in PW19 plashed. The sequence of the cDNA insert was deterdrined using the method of T7 sequencing for double strand DNA. The results showed that verc203 consisted of 195 base Pairs,and had 31 sites of restriction enzymes which were covered by 17 enzymes.Homological analysis of verc 203 and each gene sequence involved in EMBL(European Molecular Biology library )data was done in a computer with FASP method. It was found to be not homologous with verc 203 because the highest score in the 20 genes with highest scores is 42 only, far lower than 50 which is the lowest score as the standard of homology between sequences in the FASP method. On the basis of the results of homologlcal analysis for each gene in EMBL, we conclude that verc 203 may be the cDNA clone of a new gene in higher plants, and its function is related to vernalization in winter wheat.
文摘Ver203 ,a gene related to vernalization in winter wheat was cloned by the strategy of differential screening and RACE PCR. Its function was investigated by antisense transformation in both wheat and Arabidopsis. A number of transgenic plants were obtained and their response to vernalization treatment was characterized. For the plants in the 6 lines in which the antisense construct was expressed efficiently, flowering stage was significantly delayed. The developmental pattern of inflorescence was also changed in the transgenic plants of wheat and Arabidopsis . The upper spikes of transgenic plants were degenerated in wheat, and the florescence of transgenic plants became terminal flower in Arabidopsis . This suggests that the VER203 type of proteins may play an important role in floral initiation and flower development in winter wheat and Arabidopsis.
