Deep proteome profiling from trace biological samples are needed for important biomedical applications[1],but the sample losses from traditional sample preparation procedures are a major limitation[2].Therefore,consid...Deep proteome profiling from trace biological samples are needed for important biomedical applications[1],but the sample losses from traditional sample preparation procedures are a major limitation[2].Therefore,considerable efforts have been made to improve it[3,4].Filter-aided sample preparation(FASP)developed by Mann’s group[5]is recognized one of the most popular methods,by which protein extraction,reduction,alkylation,and digestion are performed on a commercial spin filter.展开更多
基金supported by the National Key Research and Development Program of China(2020YFE0202200,2017YFA0505003,and 2018YFC0910202)the National Natural Science Foundation of China(21974136,21725506,and 21775149)+1 种基金Talent Innovation Support Program of Dalian(2019CT07)Innovation Program of Science and Research from Dalian Institute of Chemical Physics,Chinese Academy of Sciences(DICP I202143)。
文摘Deep proteome profiling from trace biological samples are needed for important biomedical applications[1],but the sample losses from traditional sample preparation procedures are a major limitation[2].Therefore,considerable efforts have been made to improve it[3,4].Filter-aided sample preparation(FASP)developed by Mann’s group[5]is recognized one of the most popular methods,by which protein extraction,reduction,alkylation,and digestion are performed on a commercial spin filter.
