The aim of this study was to investigate the effect of 30 min forebrain ischemia,followed by120 min reperfusion on extracellular fluid(ECF)levels of dopamine(DA),norepinephrine(NE),serotonin(5-HT)and their metabolites...The aim of this study was to investigate the effect of 30 min forebrain ischemia,followed by120 min reperfusion on extracellular fluid(ECF)levels of dopamine(DA),norepinephrine(NE),serotonin(5-HT)and their metabolites,homovanillic acid(HVA)and 5-hydroxyindoleacetic acid(5-HIAA)in the striatum of gerbils,so as to obtain furtherinformation on the mechanism of Radix Salviae Miltiorrhizae(RSM)-inducedneuroprotection.Microdialysis was used to sample the extracellular space.Dialysate wasmeasured by high performance liquid chromatography with electrochemical detector(HPLC-ED).ECF DA,NE levels increased from basal levels by 282,227 and 221 folds,by9.14,8.51 and 8.25 folds,respectively for the three ischemic duration(0-10;11-20;21-30min).ECF DA,NE,5-HT levels in the RSM-treated group were significantly decreased ascompared with those in the control group during ischemia(P【0.01).The results suggestedthat monoamine neurotransmitters were involved in ischemic neuron damage directly orindirectly;and that RSM plays a protective role during cerebral ischemia by attenuating thedysfunctions of monoamine neurotransmitters.展开更多
文摘The aim of this study was to investigate the effect of 30 min forebrain ischemia,followed by120 min reperfusion on extracellular fluid(ECF)levels of dopamine(DA),norepinephrine(NE),serotonin(5-HT)and their metabolites,homovanillic acid(HVA)and 5-hydroxyindoleacetic acid(5-HIAA)in the striatum of gerbils,so as to obtain furtherinformation on the mechanism of Radix Salviae Miltiorrhizae(RSM)-inducedneuroprotection.Microdialysis was used to sample the extracellular space.Dialysate wasmeasured by high performance liquid chromatography with electrochemical detector(HPLC-ED).ECF DA,NE levels increased from basal levels by 282,227 and 221 folds,by9.14,8.51 and 8.25 folds,respectively for the three ischemic duration(0-10;11-20;21-30min).ECF DA,NE,5-HT levels in the RSM-treated group were significantly decreased ascompared with those in the control group during ischemia(P【0.01).The results suggestedthat monoamine neurotransmitters were involved in ischemic neuron damage directly orindirectly;and that RSM plays a protective role during cerebral ischemia by attenuating thedysfunctions of monoamine neurotransmitters.