目的 :将rBCG IL 2应用于动物实验 ,观察其对肿瘤生长和免疫功能的影响。方法 :采用基因工程技术构建rBCG IL 2 ,用黑色素瘤细胞株B16接种于小鼠小腿外侧皮下 ,再用rBCG IL 2局部治疗 ,2周后处死小鼠 ,用ELISA法检测脾淋巴细胞培养上清...目的 :将rBCG IL 2应用于动物实验 ,观察其对肿瘤生长和免疫功能的影响。方法 :采用基因工程技术构建rBCG IL 2 ,用黑色素瘤细胞株B16接种于小鼠小腿外侧皮下 ,再用rBCG IL 2局部治疗 ,2周后处死小鼠 ,用ELISA法检测脾淋巴细胞培养上清中IL 2 ,IFN γ和GM CSF的含量和肉眼及镜下观察肿瘤生长和局部免疫效应细胞浸润情况。结果 :rBCG IL 2对肿瘤生长的抑制作用明显优于对照组 ,肿瘤细胞周围有较多的巨噬细胞和淋巴细胞浸润 ,肿瘤细胞减少。同时rBCG IL 2还能诱导机体产生IL 2和IFN γ。结论 :rBCG IL 2能通过诱导局部免疫效应细胞浸润和机体细胞因子的产生 ,抑制肿瘤生长 。展开更多
Endometriosis (EMT) is one of the difficult and complicated cases in gynecology, and its incidence shows a tendency to go up year by year. It is mainly manifested in clinic as dysmenorrhea, acyesis, irregular mens... Endometriosis (EMT) is one of the difficult and complicated cases in gynecology, and its incidence shows a tendency to go up year by year. It is mainly manifested in clinic as dysmenorrhea, acyesis, irregular menstruation and pelvic lumps. Dysmenorrhea is the most characteristic symptom of endometriosis and its relief has important significance in the treatment of the disease. Certain hormonal drugs, such as danazol and gestrinone, exhibit convincing therapeutic effects but with obvious adverse actions. Operative therapy has also some limitations. Since 1997, the disorder has been treated by our special department for endometriosis with ear acupuncture therapy. Meanwhile, a group of patients were treated as controls with the decoction of traditional Chinese drugs for activating blood circulation and removing stasis. The results are reported as follows.……展开更多
文摘 Endometriosis (EMT) is one of the difficult and complicated cases in gynecology, and its incidence shows a tendency to go up year by year. It is mainly manifested in clinic as dysmenorrhea, acyesis, irregular menstruation and pelvic lumps. Dysmenorrhea is the most characteristic symptom of endometriosis and its relief has important significance in the treatment of the disease. Certain hormonal drugs, such as danazol and gestrinone, exhibit convincing therapeutic effects but with obvious adverse actions. Operative therapy has also some limitations. Since 1997, the disorder has been treated by our special department for endometriosis with ear acupuncture therapy. Meanwhile, a group of patients were treated as controls with the decoction of traditional Chinese drugs for activating blood circulation and removing stasis. The results are reported as follows.……
