O^6-甲基鸟嘌呤DNA转移酶在神经胶质瘤中的表达及其与亚硝脲耐药的关系

目的 :探讨O6 甲基鸟嘌呤DNA转移酶 (MGMT)在人脑神经胶质瘤中的表达情况及其与亚硝脲耐药之间的关系。方法 :采用免疫组化法对人脑神经胶质瘤石蜡标本中MGMT进行了检测 ,并与临床应用亚硝脲的疗效评价结果进行了比较。结果 :116例神经胶质瘤中MGMT阳性表达率为 6 7.2 %。MGMT阳性表达率最高者为髓母细胞瘤 (10 0 % ) ,表达率最低者为少枝胶质细胞瘤 (35 .7% )。在使用亚硝脲治疗的患者中 ,MGMT阳性表达与亚硝脲化疗效果呈显著负相关 (P <0 .0 5 )。肿瘤细胞的耐药程度与MGMT表达强度无关 ,只与其是否阳性有关。结论 :不同病理类型的神经胶质瘤对亚硝脲的耐药和敏感性明显不同。化疗前检测神经胶质瘤MGMT的表达情况 ,对预测化疗敏感性、指导临床使用亚硝脲化疗具有重要意义。

MGMT蛋白的表达与乳腺癌预后关系初探

目的:探讨MGMT蛋白在乳腺癌中的表达及其与乳腺癌预后的关系。方法:应用免疫组化法检测135例乳腺癌患者MGMT蛋白的表达,并探讨其与乳腺癌临床特征及预后的关系。结果:135例乳腺癌中MGMT蛋白总阳性率为84.4 %(114/135),但存在异质性。MGMT蛋白的阳性表达与患者年龄、月经状态、原发肿瘤大小、淋巴结转移情况及肿瘤的临床分期无明显相关性(P>0.05),与生存期无明显相关性(P>0.05),但与ER状况相关(P<0.025)。结论:MGMT蛋白的表达不能作为乳腺癌的一个预后指标。

导入人HeLa—S3DNA改善AT细胞对γ线辐射敏感性的初步研究

人毛细血管扩张性共济失调(Ataxia tanglectasia,简称AT)是一种常染色体隐性遗传病,患者对Υ线,X线高度敏感,神经功能异常,免疫功能部分缺陷,染色体崎变率高,易诱发肿瘤。AT患者的皮肤成纤维细胞是已知对电离辐射敏感的DNA修复缺陷的人细胞株。

Construction of serial deletants and chimeras of multi-kringle containing molecules and primary analysis of their functions

In comparison of amino acid sequences of 4 kringles of both macrophage stimulating protein (MSP) and hepatocyte growth factor (HGF), consensus motif sequence was determined. According to this consensus sequence, a pair of universal primers were designed. In combination with specific upstream or downstream primer of MSP or HGF respectively, serial fragments containing variant number of kringle (from 1 to 4) can be obtained by once PCR. By ligating the C terminal and N terminal fragments with different combination, serial deletants and chimeras of MSP and HGF were constructed. Sequence analysis showed that the degeneracy for universal primers and the sequences of those constructed deletants and chimeras are desired. Biological assay of these deletants revealed that wild type MSP can inhibit the growth of some tumor cell lines and that kringle 1 of MSP is essential for function as that of HGF.