应用Fe^(3+)和Al^(3+)溶液改良戊二醛处理牛心包瓣的实验研究

应用三氯化铁（FeCl3）和三氯化铝（AlCl3）溶液对戊二醛（GA）处理的牛心包瓣进行改良处理。探讨处理后牛心包力学特性（热缩温度、应力保留值、断裂极限拉应力及断裂应变）及在大鼠皮下钙化情况。结果表明：AlCl3及FeCl3改良处理组牛心包热收缩温度与GA组心包无显著性差异（P＜0．05），较未处理磷酸缓冲液（PBS）组有极显著提高（P＜0．01）。改良处理不影响GA处理心包的力学效应，各力学指标与GA组比较无显著差异（均为P＞0．05），FeCl3、AlCl3。改良处理极显著抑制GA组心包钙化发生（P＜0．01）。大鼠皮下埋植期间，Fe(3+)、Al(3+)改良处理组心包内Al(3+)、Fe(3+)无明显丢失。

Experimental Study on Modified Treatment andEndothelialization of Bovine Pericardial Valves

The purpose of this study were to confirm whether the modified treatment with L-glutamic acid could attenuate the calcification of the GA-fixed valves and improve its biocompatibility. Pericardial valves were routinely treated with GA and valves were treated with GA and 8% L-glutamic acid. The valves treated with these methods were implanted subcutaneously in rats. Calcium deposits of the valves collected at the 7th, 21st, 60th, 90th day were assessed by atomic absorption spectroscopy, and the pathologic changes were examined by light and electron microscopy. Cultured endothelial cells (ECs ) were seeded onto the valves. The cell counts were determined at the 1st. 4th, 7th, 10th day after seeding. PGI2 in culture medium was tested at the 10th day. Transmission and scanning electron microscopy were used to observe the growth of ECs on the valves.Results showed that subsequent treatment with L-glutamic acid could significantly mitigate calcification of bovine pericardial valves as compared with simple GAfixed valves (P<0. 01). ECs seeded on the GA treated valves died within 4 days.On the valves treated by modified method, ECs could proliferate and release PGI2. It is concluded that treatment with L- glutamic acid can markedly inhibit the calcification and improve the biocompatibility of bioprosthetical valves.

超滤法对体外循环心脏手术病理生理的影响

超滤法(ultrafiltration)是在一定跨膜压作用下,血液通过高通透膜滤器,以对流方式滤过血浆水份和中小分子物质。体外循环心脏手术超滤法能浓缩血液,逆转血液稀释的不良影响。近年来国内外已有报道本文综述超滤法的病理生理影响。一、减少体内液体潴留体外循环血液稀释具有不少优点,但它常引起体内液体潴留。

高钾心肌麻痹液和低温对心肌快反应细胞电生理特性的影响

应用玻璃微电极记录心肌细胞动作电位的方法来研究高钾心肌麻痹液和低温对心肌细胞电生理特性的影响，结果提示豚鼠乳头状肌的静息电位绝对值变小，使其部分标本快钠通道完全失活而不能产生快反应动作电位。一部分标本快钠通道没完全失活仍可产生低振幅短时程动作电位伴机械收缩，还有一部分标本慢钙通道可被激活产生慢反应动作电位，并伴机械收缩，异丙肾上腺素促进这种慢钙通道的开放。低温明显延长豚鼠乳头状肌动作电位时程、收缩时程，增加收缩幅度、静态张力。

前列腺素与心血管外科

近年来,前列腺素(PG)及其衍生物日益受到临床工作者的重视,在心内科方面已用于治疗心绞痛、心肌梗塞、心力衰竭、动脉粥样硬化等疾病。本文概述 PG 与心血管外科有关的基础研究和临床应用的进展。前列腺素的分类、合成与生物学效应(一)前列腺素的生物合成与分类 PGs 是一类由二十碳多不饱和脂肪酸衍化而来的化合物。

生物瓣抗钙化的实验研究

生物瓣抗钙化的实验研究傅平蓝鸿钧蔡俊坚管汉屏本实验研究应用环氧化物ＤｅｎａｃｏｌＥＸ－３１３（ＥＸ）对牛心包进行处理，探讨其力学特性，观察在大鼠皮下埋植后的钙化状况，并初步探讨其抗钙化机理。一、材料和方法１．牛心包获取及实验分组：无菌方法取３～４岁小...

三氯化铝改良牛心包瓣处理的研究

通过应用三氯化铝(AlCl_3)对戊二醛(Glutaraldehyde,GA)处理的牛心包瓣进行改良处理,探讨处理后牛心包力学特性及其在大鼠皮下钙化情况。方法:以AlCl_3对GA处理的牛心包瓣改良处理为实验组,以单纯GA处理(GA组)和未处理(PBS组)作对照。测定各组心包热收缩温度和各力学指标,并将各组心包片植入大鼠皮下分析钙化。结果:AlCl_3改良处理组热收缩温度高于GA处理组心包,但无显著性差异(P>0.05)。AlCl_3改良组各力学指标与GA组比较无显著差异(P>0.05),AlCl_3改良处理极显著抑制钙化发生(P<0.01)。结论:AlCl_3改良处理GA处理的牛心包瓣可提高热收缩温度,不影响其力学特性,且具良好的抗钙化性能。