A liquid chromatography-tandem mass spectroscopy(LC-MS/MS)method was developed for analysis of carbonyl compounds in cigarette mainstream smoke.The cigarette smoke was collected using a Cambridge filter treated with a...A liquid chromatography-tandem mass spectroscopy(LC-MS/MS)method was developed for analysis of carbonyl compounds in cigarette mainstream smoke.The cigarette smoke was collected using a Cambridge filter treated with acidic solution of 2,4-dinitrophenylhydrazine,then extracted with acetonitrile.The extract was diluted with acetonitrile-water and analyzed by LC-MS/MS in negative electro-spray ionization mode.Formaldehyde,acetaldehyde,acetone,acrolein,propionaldehyde,methyl ethyl ketone,butyraldehyde and crotonaldehyde in mainstream cigarette smoke were analyzed with the recovery at the range of 83% to 103%.The minimum detectable quantity for the carbonyls ranged from 0.08 to 0.28 μg/L,and relative standard deviations were below 6%.This new method had higher selectivity and sensitivity compared to conventional methods.5 kinds of foreign and domestic cigarette were analyzed.展开更多
目的:建立HPLC法测定不同产地桔梗药材中桔梗皂苷D的含量。方法:HPLC分析采用Phenomenex Luna C18色谱柱,以0.1%磷酸水溶液-乙腈为流动相进行梯度洗脱,流速0.5mL/min,检测波长210nm。结果:桔梗皂苷D分离良好,质量浓度与峰面积在测定范...目的:建立HPLC法测定不同产地桔梗药材中桔梗皂苷D的含量。方法:HPLC分析采用Phenomenex Luna C18色谱柱,以0.1%磷酸水溶液-乙腈为流动相进行梯度洗脱,流速0.5mL/min,检测波长210nm。结果:桔梗皂苷D分离良好,质量浓度与峰面积在测定范围内线性关系良好(r>0.999),重现性好,平均加样回收率为100.09%,13批不同产地桔梗药材中桔梗皂苷D占药材量的平均含量为0.2101%。结论:本研究所建立的HPLC方法稳定可靠,简便易行,可用于桔梗中桔梗皂苷D的定量,为桔梗的质量评价和质量控制奠定了基础。展开更多
文摘A liquid chromatography-tandem mass spectroscopy(LC-MS/MS)method was developed for analysis of carbonyl compounds in cigarette mainstream smoke.The cigarette smoke was collected using a Cambridge filter treated with acidic solution of 2,4-dinitrophenylhydrazine,then extracted with acetonitrile.The extract was diluted with acetonitrile-water and analyzed by LC-MS/MS in negative electro-spray ionization mode.Formaldehyde,acetaldehyde,acetone,acrolein,propionaldehyde,methyl ethyl ketone,butyraldehyde and crotonaldehyde in mainstream cigarette smoke were analyzed with the recovery at the range of 83% to 103%.The minimum detectable quantity for the carbonyls ranged from 0.08 to 0.28 μg/L,and relative standard deviations were below 6%.This new method had higher selectivity and sensitivity compared to conventional methods.5 kinds of foreign and domestic cigarette were analyzed.
文摘目的:建立HPLC法测定不同产地桔梗药材中桔梗皂苷D的含量。方法:HPLC分析采用Phenomenex Luna C18色谱柱,以0.1%磷酸水溶液-乙腈为流动相进行梯度洗脱,流速0.5mL/min,检测波长210nm。结果:桔梗皂苷D分离良好,质量浓度与峰面积在测定范围内线性关系良好(r>0.999),重现性好,平均加样回收率为100.09%,13批不同产地桔梗药材中桔梗皂苷D占药材量的平均含量为0.2101%。结论:本研究所建立的HPLC方法稳定可靠,简便易行,可用于桔梗中桔梗皂苷D的定量,为桔梗的质量评价和质量控制奠定了基础。
