Objective: To investigate the transmission ratio and epidemiology of Chlamydia trachomatis (Ct) and Ureaplasma urealyticum (Uu) between regular sexual partners. Methods: We collected secretion specimens of geni-tour...Objective: To investigate the transmission ratio and epidemiology of Chlamydia trachomatis (Ct) and Ureaplasma urealyticum (Uu) between regular sexual partners. Methods: We collected secretion specimens of geni-tourinary tract from sexual partners who attended our clinic during the period from May 1, 1999 to May 31,2000 and then detected Ct and Uu in the specimens. Results: The inconsistent rate of Ct and Uu infected between sexual partners was 59.22% and 38.92% respectively. There were no correlations between pa-tients' age and the inconsistent rate of Ct and Uu in-fected (P>0.05). The most common inconsistency be-tween regular partners was seen in man with Ct infec-tion while in female with Uu infection; and in man with concurrent infection of Ct while in female only with Uu; or in man with Ct infection while in female no Ct and Uu were detected. Conclusion: The sexual transmitting ability of Ct and Uu is not as active as we have expected.展开更多
Objective:To directionally clone the ompl gene from Chlamydia trachomatis(Ct)F Genotype onto a plasmid vector for constructing a rudimentary DNA vaccine.Methods:The complete ompl gene from genomic DNA of Ct F genotype...Objective:To directionally clone the ompl gene from Chlamydia trachomatis(Ct)F Genotype onto a plasmid vector for constructing a rudimentary DNA vaccine.Methods:The complete ompl gene from genomic DNA of Ct F genotype wild species was amplified with primers designed by computer.The recombinant gene was obtained by restriction enzyme cutting,linking the gene with the plasmid vector in vitro,transforming the recombinant gene into bacteria,and extracting the DNA from the bacteria.Results:DNA extracting from the bacteria was composed of the impl gene and plasmid,which is identified by three methods of singular restrictive enzyme cutting,double restrictive enzyme cutting and PCR.Conclusion:Cloning of the ompl gene from the Ct F genotype means that a rudimentary DNA vaccine was successfully constructed.展开更多
文摘Objective: To investigate the transmission ratio and epidemiology of Chlamydia trachomatis (Ct) and Ureaplasma urealyticum (Uu) between regular sexual partners. Methods: We collected secretion specimens of geni-tourinary tract from sexual partners who attended our clinic during the period from May 1, 1999 to May 31,2000 and then detected Ct and Uu in the specimens. Results: The inconsistent rate of Ct and Uu infected between sexual partners was 59.22% and 38.92% respectively. There were no correlations between pa-tients' age and the inconsistent rate of Ct and Uu in-fected (P>0.05). The most common inconsistency be-tween regular partners was seen in man with Ct infec-tion while in female with Uu infection; and in man with concurrent infection of Ct while in female only with Uu; or in man with Ct infection while in female no Ct and Uu were detected. Conclusion: The sexual transmitting ability of Ct and Uu is not as active as we have expected.
文摘Objective:To directionally clone the ompl gene from Chlamydia trachomatis(Ct)F Genotype onto a plasmid vector for constructing a rudimentary DNA vaccine.Methods:The complete ompl gene from genomic DNA of Ct F genotype wild species was amplified with primers designed by computer.The recombinant gene was obtained by restriction enzyme cutting,linking the gene with the plasmid vector in vitro,transforming the recombinant gene into bacteria,and extracting the DNA from the bacteria.Results:DNA extracting from the bacteria was composed of the impl gene and plasmid,which is identified by three methods of singular restrictive enzyme cutting,double restrictive enzyme cutting and PCR.Conclusion:Cloning of the ompl gene from the Ct F genotype means that a rudimentary DNA vaccine was successfully constructed.
