胸腺细胞受照后发生凋落——一种与坏死不同的主动性细胞死亡.为了检测单个凋落细胞的 DNA 变化,我们建立了微凝胶电泳法.受照的大鼠胸腺细胞培养后用本法进行电泳,结果可见细胞被分为二群,一群细胞的 DNA 几乎没有泳动,另一群细胞的 D...胸腺细胞受照后发生凋落——一种与坏死不同的主动性细胞死亡.为了检测单个凋落细胞的 DNA 变化,我们建立了微凝胶电泳法.受照的大鼠胸腺细胞培养后用本法进行电泳,结果可见细胞被分为二群,一群细胞的 DNA 几乎没有泳动,另一群细胞的 DNA 则泳动成大小相似的水滴或慧星形状.DNA 泳动细胞的比例与细胞死亡的比例一致.实验说明凋落细胞的 DNA 分解十分迅速,一旦开始就很快达到一定程度.展开更多
用~3H—TdR 掺入淋巴细胞 DNA 合成法,研究11例癌症患者放疗前、后(45～65GY)外周血被 PHA、PWM 诱导淋巴细胞转化的 DNA 合成能力及其辐射效应。结果发现,癌症患者淋巴细胞DNA 合成能力较正常人明显降低;放疗后更低。放疗后癌患者血液...用~3H—TdR 掺入淋巴细胞 DNA 合成法,研究11例癌症患者放疗前、后(45～65GY)外周血被 PHA、PWM 诱导淋巴细胞转化的 DNA 合成能力及其辐射效应。结果发现,癌症患者淋巴细胞DNA 合成能力较正常人明显降低;放疗后更低。放疗后癌患者血液再经^(60)Coγ线0.25～4GY 照射后,两种诱导细胞的~3H 掺入量均依受照射剂量大小呈规律性减少。PHA 细胞 DNA 合成受抑大于 PWM细胞。展开更多
This work was supported by the National Natural Science Foundation of China (No. 3870902). Objective: To determine the NK activity of lymphocyte subsets and the effects of low dose radiation. Methods: Lymphocyte s...This work was supported by the National Natural Science Foundation of China (No. 3870902). Objective: To determine the NK activity of lymphocyte subsets and the effects of low dose radiation. Methods: Lymphocyte subsets were separated by monoclonal antibodies. The NK activity of each subset on tumor cells was detected by radioactive release method. Results: The results showed that besides NK cells, CD 4, CD 8 and B cells alone can kill tumor cells. But the cellkilling activity of NK cells appeared to be strongest. There was synergistic effect between CD 4 and NK cells. The activity of mixed lymphocytes was more than that of only one subset. The effect of low dose radiation (LDR) on NK activity of panlymphocytes or NK cells was different. Conclusion: This paper demonstrated that NK activity of mononuclear cells was called “NK activity of lymphocytes”, but it is not true. Only when NK cells were separated by monoclonal antibodies, its killer activity can be called “activity of NK cells”.展开更多
文摘胸腺细胞受照后发生凋落——一种与坏死不同的主动性细胞死亡.为了检测单个凋落细胞的 DNA 变化,我们建立了微凝胶电泳法.受照的大鼠胸腺细胞培养后用本法进行电泳,结果可见细胞被分为二群,一群细胞的 DNA 几乎没有泳动,另一群细胞的 DNA 则泳动成大小相似的水滴或慧星形状.DNA 泳动细胞的比例与细胞死亡的比例一致.实验说明凋落细胞的 DNA 分解十分迅速,一旦开始就很快达到一定程度.
文摘用~3H—TdR 掺入淋巴细胞 DNA 合成法,研究11例癌症患者放疗前、后(45～65GY)外周血被 PHA、PWM 诱导淋巴细胞转化的 DNA 合成能力及其辐射效应。结果发现,癌症患者淋巴细胞DNA 合成能力较正常人明显降低;放疗后更低。放疗后癌患者血液再经^(60)Coγ线0.25～4GY 照射后,两种诱导细胞的~3H 掺入量均依受照射剂量大小呈规律性减少。PHA 细胞 DNA 合成受抑大于 PWM细胞。
文摘This work was supported by the National Natural Science Foundation of China (No. 3870902). Objective: To determine the NK activity of lymphocyte subsets and the effects of low dose radiation. Methods: Lymphocyte subsets were separated by monoclonal antibodies. The NK activity of each subset on tumor cells was detected by radioactive release method. Results: The results showed that besides NK cells, CD 4, CD 8 and B cells alone can kill tumor cells. But the cellkilling activity of NK cells appeared to be strongest. There was synergistic effect between CD 4 and NK cells. The activity of mixed lymphocytes was more than that of only one subset. The effect of low dose radiation (LDR) on NK activity of panlymphocytes or NK cells was different. Conclusion: This paper demonstrated that NK activity of mononuclear cells was called “NK activity of lymphocytes”, but it is not true. Only when NK cells were separated by monoclonal antibodies, its killer activity can be called “activity of NK cells”.
