变温与恒温对孔雀种蛋孵化效果的试验研究

为了研究变温与恒温对孔雀种蛋孵化效果,试验采用变温与恒温对2组孔雀种蛋进行孵化研究。结果表明:在蓝孔雀受精种蛋孵化率性状上,试验Ⅰ组(变温组)显著高于试验Ⅱ组(恒温组),说明孵化期间采取前期高、中期平、后期略低、出雏期稍高的变温措施,符合孔雀胚龄发育所需的最适温度变化规律,有利于孔雀受精蛋的孵化。

Effects of Varying Temperature and Constant Temperature on Hatching Result of Fertilized Peacock Eggs

In order to investigate the effect of temperature on hatching result of fertilized peacock eggs, they were hatched under conditions of varying temperature and constant temperature respectively. The result shows that the hatching rate in the varying temperature treatment group （Group Ⅰ） is higher than that in the constant temperature treatment group （Group Ⅱ）. It suggests that the varying temperature treatment （high temperature in the early period, medium temperature in the middle period, slightly lower temperature in the late period, and slightly higher temperature in the hulling period） is in line with the optimum temperature variation in the hatching of fertilized peacock eggs, contributing the hatching of fertilized peacock eggs.

蔗糖诱导拟南芥突变体rhd3合成花青素及基因表达分析

【目的】研究拟南芥内质网蛋白基因AtRHD3(root hair defective 3)在花青素合成中的作用。【方法】以蔗糖为诱导因素,比较拟南芥野生型Col-0及突变体rhd3中花青素的积累量,并通过实时荧光定量PCR技术分析花青素合成通路中基因的表达差异。【结果】以5%蔗糖(sucrose,Suc)为诱导因素,随培养基中蔗糖浓度的增加,野生型Col-0及突变体rhd3中花青素含量逐渐增加,且突变体rhd3中花青素积累量显著高于野生型Col-0,表明AtRHD3在蔗糖诱导的花青素合成中起负调控作用。与野生型Col-0相比,在突变体rhd3中结构基因AtDFR、AtANS、AtUF3GT和正调控基因AtPAP1、AtPAP2、AtTT8、AtTTG1表达水平较高,而负调控基因AtMYBL2表达水平较低。【结论】在蔗糖诱导的拟南芥花青素合成中,AtRHD3通过影响花青素合成通路结构基因和调控基因的表达水平负调控花青素的合成。