[Objective] The aim of this study was to extract RNA,DNA and protein from plants by using modified one step method.[Method]CTAB was used for the modifying of Biozol-based one step extraction method,which was then used...[Objective] The aim of this study was to extract RNA,DNA and protein from plants by using modified one step method.[Method]CTAB was used for the modifying of Biozol-based one step extraction method,which was then used for the isolation of RNA,DNA and protein from the roots of maize（Zea mays L.）,soybean（Glycine max L.）,alfalfa（Medicago sativa L.）and cucumber（Cucumis sativus L.）.And the heme oxygenase-1 gene（HO-1）and calcium-dependent protein kinase-1 gene（CDPK1）,as well as HO-1 protein were identified.[Result]The result of UV spectrophotometer determination and agarose gel electrophoresis analysis showed that the purity of obtained RNA and DNA sample was relatively high;HO-1 and CDPK1 gene exhibited the positive signals checked by RT-PCR;the extracted protein could be used for western blotting analysis;the whole isolation procedure cost 3 h.[Conclusion]This method can be used as a good reference for researcher and will offer a better value with a wide range of applications.展开更多
电力系统稳定器(Power System Stabilizer,PSS)通过调节励磁电流进而调整发电机内电势,以实现对有功低频振荡的抑制。由于系统中除了PSS控制器外,还存在各种FACTS元件和储能装置,各控制器参数间需要协调控制以提高系统阻尼。为此,对PSS...电力系统稳定器(Power System Stabilizer,PSS)通过调节励磁电流进而调整发电机内电势,以实现对有功低频振荡的抑制。由于系统中除了PSS控制器外,还存在各种FACTS元件和储能装置,各控制器参数间需要协调控制以提高系统阻尼。为此,对PSS与储能装置间的协调机制进行了分析,确定了相应的协调控制策略,同时建立了相应的参数优化模型,利用细菌群体趋药性(BCC)算法对PSS和储能控制器进行了参数优化。最后基于电力系统分析综合程序(PSASP),以4机2区域系统模型为例,搭建相关控制器模型,对协调优化方法进行了验证,特征值分析及时域仿真结果表明,本文所提优化策略能够提高系统阻尼,有效抑制低频振荡。展开更多
基金Supported by Opening Program of Physiologial Key Laboratory for Cultivation of Tropical Crops in Hainan Province(Key Opening Bio-logial Laboratory of Rubber of Ministry of Agriculture/Breeding Basein State and Provincial Key Laboratory)(KLOF0907)Major and Special Program for Cultivation of Genetically Modified New Varieties(No.2009ZX08012-014B)+1 种基金National Natural Science Foundation of China(No.30971711)Natural Science Foundation in Jiangsu Province(No.BK2009309)~~
文摘[Objective] The aim of this study was to extract RNA,DNA and protein from plants by using modified one step method.[Method]CTAB was used for the modifying of Biozol-based one step extraction method,which was then used for the isolation of RNA,DNA and protein from the roots of maize（Zea mays L.）,soybean（Glycine max L.）,alfalfa（Medicago sativa L.）and cucumber（Cucumis sativus L.）.And the heme oxygenase-1 gene（HO-1）and calcium-dependent protein kinase-1 gene（CDPK1）,as well as HO-1 protein were identified.[Result]The result of UV spectrophotometer determination and agarose gel electrophoresis analysis showed that the purity of obtained RNA and DNA sample was relatively high;HO-1 and CDPK1 gene exhibited the positive signals checked by RT-PCR;the extracted protein could be used for western blotting analysis;the whole isolation procedure cost 3 h.[Conclusion]This method can be used as a good reference for researcher and will offer a better value with a wide range of applications.
文摘电力系统稳定器(Power System Stabilizer,PSS)通过调节励磁电流进而调整发电机内电势,以实现对有功低频振荡的抑制。由于系统中除了PSS控制器外,还存在各种FACTS元件和储能装置,各控制器参数间需要协调控制以提高系统阻尼。为此,对PSS与储能装置间的协调机制进行了分析,确定了相应的协调控制策略,同时建立了相应的参数优化模型,利用细菌群体趋药性(BCC)算法对PSS和储能控制器进行了参数优化。最后基于电力系统分析综合程序(PSASP),以4机2区域系统模型为例,搭建相关控制器模型,对协调优化方法进行了验证,特征值分析及时域仿真结果表明,本文所提优化策略能够提高系统阻尼,有效抑制低频振荡。
