In the past five years, the therapy of colorectal carcinoma by mouse monoclonal antibody (mAb) 17 1A has proven that the tumour associated antigen (17 1A antigen) recognized by mAb 17 1A serves as a useful antigen in...In the past five years, the therapy of colorectal carcinoma by mouse monoclonal antibody (mAb) 17 1A has proven that the tumour associated antigen (17 1A antigen) recognized by mAb 17 1A serves as a useful antigen in the detection of tumor cells and adjuvant therapy of colorectal carcinoma. In this study, we investigated modulation of 17 1A antigen expression by human interferon α (IFN α) using flow cytometry analysis. IFN α enhances 17 1A antigen expression on HT 29 cell surfaces by about 40%. Using M79 sepharose column (M79: mouse mAb to 17 1A antigen), a protein (p33) with a relative molecular mass of 3 3×10 4 was isolated from HT 29 and HCT (colon carcinoma cell lines) by affinity chromatography analysis and SDS PAGE under reducing and non reducing conditions. P33 was identified as 17 1A antigen by silver staining and ELISA assay. The antibodies 17 1A and M79 could recognize the protein p33. These results indicate that HT29 and HCT cell lines all express a relative molecular mass of 3 3×10 4 tumor associated antigen whose expression could be up modulated by human IFN α.展开更多
This paper presents the purification of a monoclonal IgM antibody against human tumor associated antigen Lewis\|Y by ion exchange chromatography and gel filtration. Enzyme\|linked immunosorbent assay (ELISA) and SDS\|...This paper presents the purification of a monoclonal IgM antibody against human tumor associated antigen Lewis\|Y by ion exchange chromatography and gel filtration. Enzyme\|linked immunosorbent assay (ELISA) and SDS\|polyacrylamide gel electrophoresis (PAGE) were used to identify purified IgM antibody. In flow cytometry analysis, the purified IgM antibody recognizes human breast tumor cell line MCF\|7 which expresses Lewis\|Y antigen. This work presents a new way for the purification of murine monoclonal IgM antibody.展开更多
A is a tumor associated antigen. Monoclonal antibody (mAb) against 17 1A has been used in adjuvant therapy of colorectal carcinoma. Using mAb against 17 1A antigen on affinity chromatography, a novel putative tumor ...A is a tumor associated antigen. Monoclonal antibody (mAb) against 17 1A has been used in adjuvant therapy of colorectal carcinoma. Using mAb against 17 1A antigen on affinity chromatography, a novel putative tumor associated antigen (P50) whose relative molecular mass is 5 0×10 4 has been isolated from human colorectal tumor tissues which are recognized by mAbs 17 1A and M79, while the relative molecular mass of 17\|1A antigen isolated from several colorectal tumor cell lines is 3 3×10 4. P50 was recognized by mAbs 17 1A and M79 which are specific mAbs against 17 1A antigen.展开更多
Using flow cytometry analysis with human type Ⅰinterferons (IFN) (IFN α and β) and IFN α/β receptor antibody (ligand binding and antibody binding), we compared human IFN α/β receptor expressions on human H...Using flow cytometry analysis with human type Ⅰinterferons (IFN) (IFN α and β) and IFN α/β receptor antibody (ligand binding and antibody binding), we compared human IFN α/β receptor expressions on human H9 (T cells), Raji (B cells) and U937 (monocyte cells). The results indicated that H9, Raji and U937 cells all expressed IFN α/β receptor on their cell surfaces and the Raji cells showed the highest level of expression. The flow cytometry analysis using the antiserum to human IFN α/β receptor more clearly showed a strong IFN α/β receptor expression on Raji cells and a weak expression on H9 and U937 cells. These results indicate that B cells (Raji) expressed more receptor molecules on their cell surface than on T cells (H9) and on monocytes (U937), and the number of receptor molecules expressed on the cells is enough to be measured with flow cytometry.展开更多
文摘In the past five years, the therapy of colorectal carcinoma by mouse monoclonal antibody (mAb) 17 1A has proven that the tumour associated antigen (17 1A antigen) recognized by mAb 17 1A serves as a useful antigen in the detection of tumor cells and adjuvant therapy of colorectal carcinoma. In this study, we investigated modulation of 17 1A antigen expression by human interferon α (IFN α) using flow cytometry analysis. IFN α enhances 17 1A antigen expression on HT 29 cell surfaces by about 40%. Using M79 sepharose column (M79: mouse mAb to 17 1A antigen), a protein (p33) with a relative molecular mass of 3 3×10 4 was isolated from HT 29 and HCT (colon carcinoma cell lines) by affinity chromatography analysis and SDS PAGE under reducing and non reducing conditions. P33 was identified as 17 1A antigen by silver staining and ELISA assay. The antibodies 17 1A and M79 could recognize the protein p33. These results indicate that HT29 and HCT cell lines all express a relative molecular mass of 3 3×10 4 tumor associated antigen whose expression could be up modulated by human IFN α.
基金the Ministry of Education of China! (No .1 996 4 6 6 )
文摘This paper presents the purification of a monoclonal IgM antibody against human tumor associated antigen Lewis\|Y by ion exchange chromatography and gel filtration. Enzyme\|linked immunosorbent assay (ELISA) and SDS\|polyacrylamide gel electrophoresis (PAGE) were used to identify purified IgM antibody. In flow cytometry analysis, the purified IgM antibody recognizes human breast tumor cell line MCF\|7 which expresses Lewis\|Y antigen. This work presents a new way for the purification of murine monoclonal IgM antibody.
基金the Ministry of Education of China !(No .1 996 4 6 6 )
文摘A is a tumor associated antigen. Monoclonal antibody (mAb) against 17 1A has been used in adjuvant therapy of colorectal carcinoma. Using mAb against 17 1A antigen on affinity chromatography, a novel putative tumor associated antigen (P50) whose relative molecular mass is 5 0×10 4 has been isolated from human colorectal tumor tissues which are recognized by mAbs 17 1A and M79, while the relative molecular mass of 17\|1A antigen isolated from several colorectal tumor cell lines is 3 3×10 4. P50 was recognized by mAbs 17 1A and M79 which are specific mAbs against 17 1A antigen.
文摘Using flow cytometry analysis with human type Ⅰinterferons (IFN) (IFN α and β) and IFN α/β receptor antibody (ligand binding and antibody binding), we compared human IFN α/β receptor expressions on human H9 (T cells), Raji (B cells) and U937 (monocyte cells). The results indicated that H9, Raji and U937 cells all expressed IFN α/β receptor on their cell surfaces and the Raji cells showed the highest level of expression. The flow cytometry analysis using the antiserum to human IFN α/β receptor more clearly showed a strong IFN α/β receptor expression on Raji cells and a weak expression on H9 and U937 cells. These results indicate that B cells (Raji) expressed more receptor molecules on their cell surface than on T cells (H9) and on monocytes (U937), and the number of receptor molecules expressed on the cells is enough to be measured with flow cytometry.
