To establish a TaqMan-based real-time RT-PCR method for detection of canine distemper virus,the pair of primers and the TaqMan-based probe were designed according to the M protein genes of canine distemper virus strai...To establish a TaqMan-based real-time RT-PCR method for detection of canine distemper virus,the pair of primers and the TaqMan-based probe were designed according to the M protein genes of canine distemper virus strains.The reactive conditions were optimized to improve the sensitivity and specificity of the method.The specificity and sensitivity of the method were high.Canine distemper virus in the thirty-eight samples had been tested by the TaqMan-based real-time RT-PCR,the common RT-PCR and electron microscopy.The sensitivity of the TaqMan-based real-time RT-PCR was higher than the common RT-PCR and electron microscopy.This suggests that the method may be used in clinical diagnosis,epizootic study and laboratory research.展开更多
文摘To establish a TaqMan-based real-time RT-PCR method for detection of canine distemper virus,the pair of primers and the TaqMan-based probe were designed according to the M protein genes of canine distemper virus strains.The reactive conditions were optimized to improve the sensitivity and specificity of the method.The specificity and sensitivity of the method were high.Canine distemper virus in the thirty-eight samples had been tested by the TaqMan-based real-time RT-PCR,the common RT-PCR and electron microscopy.The sensitivity of the TaqMan-based real-time RT-PCR was higher than the common RT-PCR and electron microscopy.This suggests that the method may be used in clinical diagnosis,epizootic study and laboratory research.
